Applied Microbiology

A.Y. 2019/2020
6
Max ECTS
48
Overall hours
SSD
BIO/19
Language
Italian
Learning objectives
In this course students will acquire basic knowledge of microbiology. The students will learn structure and functions of the prokaryotic cell and will then be confronted with the metabolic and physiological diversity of prokaryotes. Key concepts on genetics will be also offered to students. Overall these concepts represent tools to understand the applications of microbiology in the medical, pharmaceutical and diagnostic fields as well as in food industry.
Expected learning outcomes
On successful completion of this course the student will have acquired basic knowledge in microbiology and will know in details the functioning of the prokaryotic cell. Moreover, the student will become familiar with the applications of microbiology in the medical and industrial fields.
Course syllabus and organization

Linea AK

Responsible
Lesson period
First semester
Course syllabus
1) Overview of microbiology and its applications.
2) Overview of cell structure: prokaryotes and eukaryotes. Bacteria and Archaea. Cytoplasmic membrane, structure and function. Bacterial transport systems. Cell wall of prokaryotes. Outer membrane of Gram-negative bacteria. Capsules. Flagella and bacterial motility. Surface structure for adhesion: pili. The nucleoid. Structure and organization of bacterial chromosome. Cytoplasm. Ribosomes. Cell inclusions. Endospores.
3) Microbial growth and microbial growth control. Principles of microbial nutrition. Liquid and solid media, growth conditions. Methods for sterilization. Disinfectants and antiseptics.
4) Nutrition and metabolism. Overview of metabolism. Fermentation and substrate level phosphorylation. Aerobic and anaerobic respiration. Chemiolithotrophy. Photosynthesis: anoxygenic photosynthesis. Autotrophic CO2 fixation. Nitrogen metabolism. Nitrogen fixation.
5) Microbial genetics. Mutations and mutants. Horizontal gene transfer mechanisms. Overview of regulation of gene expression.
6) Viruses. Overview of bacterial viruses. Lytic cycle and lysogeny.
7) Selected examples of microorganisms relevant in clinic and industry
8) Antibiotics. Structure and mechanisms of action. Minimal inhibitory concentration (MIC). Overview on resistance mechanisms.
Prerequisites for admission
Students need to possess basic knowledge of biology, human anatomy and physiology
Teaching methods
Frontal lessons and classroom exercises
Bibliography
Galli-Dehò Biologia dei Microrganismi Casa Editrice Ambrosiana, 3ed 2018
Course slides on Ariel website
Assessement methods and criteria
Written exam: multiple choice (20 questions) and open questions (3 questions and 1 exercise). Maximal score related to complexity of questions. Final score is the sum of scores obtained in the different sections of the exam.
BIO/19 - MICROBIOLOGY - University credits: 6
Lessons: 48 hours
Professor: Sperandeo Paola

Linea LZ

Responsible
Lesson period
First semester
Course syllabus
1) Overview of microbiology and its applications.
2) Overview of cell structure: prokaryotes and eukaryotes. Bacteria and Archaea. Cytoplasmic membrane, structure and function. Bacterial transport systems. Cell wall of prokaryotes. Outer membrane of Gram-negative bacteria. Capsules. Flagella and bacterial motility. Surface structure for adhesion: pili. The nucleoid. Structure and organization of bacterial chromosome. Cytoplasm. Ribosomes. Cell inclusions. Endospores.
3) Microbial growth and microbial growth control. Principles of microbial nutrition. Liquid and solid media, growth conditions. Methods for sterilization. Disinfectants and antiseptics.
4) Nutrition and metabolism. Overview of metabolism. Fermentation and substrate level phosphorylation. Aerobic and anaerobic respiration. Chemiolithotrophy. Photosynthesis: anoxygenic photosynthesis. Autotrophic CO2 fixation. Nitrogen metabolism. Nitrogen fixation.
5) Microbial genetics. Mutations and mutants. Horizontal gene transfer mechanisms. Overview of regulation of gene expression.
6) Viruses. Overview of bacterial viruses. Lytic cycle and lysogeny.
7) Selected examples of microorganisms relevant in clinic and industry
8) Antibiotics. Structure and mechanisms of action. Minimal inhibitory concentration (MIC). Overview on resistance mechanisms.
Prerequisites for admission
Physiology and Applied Biology
Teaching methods
Lessons and classroom exercises
Bibliography
Galli-Dehò Biologia dei Microrganismi Casa Editrice Ambrosiana, 3ed 2018
Slides on e-learning platform
Assessement methods and criteria
Written exam: multiple choice and open questions. Maximal score related to complexity of questions. Final score is the sum of scores obtained in the different sections of the exam. Time: 2 hours
BIO/19 - MICROBIOLOGY - University credits: 6
Lessons: 48 hours
Professor: Polissi Alessandra
Professor(s)
Reception:
By email appointment
Via Balzaretti, 9. 4th floor