Experimental Nutrition

A.Y. 2019/2020
5
Max ECTS
42
Overall hours
SSD
AGR/18
Language
Italian
Learning objectives
The aim of the course is to provide students the principal methodologies applied to nutritional studies, also providing the skills to develop a diet for laboratory animals.
Expected learning outcomes
At the end of the course the student will possess:
-in-depth knowledge of animal nutrition and models applied to nutritional studies;
-knowledge of nutrigenomics and nutri-epigenetics;
-knowledge of laboratory animal diets.
Furthermore the student will be able to
- use and apply the main molecular biology techniques into nutritional studies;
- use in vitro digestion protocols applied to feed and food ;
-design a diet for laboratory animals.
Course syllabus and organization

Unique edition

Responsible
Lesson period
First semester
Course syllabus
ATTENDING STUDENTS
Prof. Pinotti
Lectures (12h)
- lab animal nutrition in biomedicine research: how to formulate experimental diets
- Methods in nutritional studies
-Non conventional feed
-Former food / Food waste / ecofeed
-Insects
- Methods for nutritional and functional evaluation

Practical training: 12h
-Methods for characterization of new ingredients
-In vitro feed/food digestibility
-Database consultation
-Technical visits

Prof. Giromini
Lectures (6h)
-Characterization of the main in vitro models applied to nutritional studies
-Application of static and dynamic digestion and absorption models
-Bioactive and functional molecules in the diet
-Epigentics applied to nutrition

Practical training: 12h
-Molecular biology techniques applied to nutritional studies

NON-ATTENDING STUDENTS
Prof. Pinotti
Lectures (12h)
- lab animal nutrition in biomedicine research: how to formulate experimental diets
- Methods in nutritional studies
-Non conventional feed
-Former food / Food waste / ecofeed
-Insects
- Methods for nutritional and functional evaluation

Practical training: 12h
-Methods for characterization of new ingredients
-In vitro feed/food digestibility
-Database consultation
-Technical visits

Prof. Giromini
Lectures (6h)
-Characterization of the main in vitro models applied to nutritional studies
-Application of static and dynamic digestion and absorption models
-Bioactive and functional molecules in the diet
-Epigentics applied to nutrition

Practical training: 12h
-Molecular biology techniques applied to nutritional studies
Prerequisites for admission
n/a
Teaching methods
The course includes both Theoretical and practical classess.
Seminars and educational visits into industries will complete the course activities
Bibliography
Giromini, C., Cheli, F., Rebucci, R., & Baldi, A. (2019). Invited review: Dairy proteins and bioactive peptides: Modeling digestion and the intestinal barrier. Journal of dairy science, 102(2), 929-942.

Giromini, C., Lovegrove, J. A., Givens, D. I., Rebucci, R., Pinotti, L., Maffioli, E., ... & Baldi, A. (2019). In vitro-digested milk proteins: Evaluation of angiotensin-1-converting enzyme inhibitory and antioxidant activities, peptidomic profile, and mucin gene expression in HT29-MTX cells. Journal of dairy science, 102(12), 10760-10771.

Baldi et al.,
Biological effects of milk proteins and their peptides with emphasis on those related to the gastrointestinal ecosystem. Journal: Journal of Dairy Research / Volume 72 / Issue S1 / August 2005

Pinotti L., & A. Baldi, et al., Nutritional evaluation of former food products (ex-food) intended for pig nutrition, Food Additives & Contaminants: Part A
doi.org/10.1080/19440049.2017.1306884

Pinotti, L., Ottoboni, M., Luciano, A., Savoini, G., Cattaneo, D., & Tretola, M. (2019). Ex-food in animal nutrition: potentials and challenges. In EAAP Scientific Series (p. 1064580). Wageningen Academic Publishers.

Insects as a source of nutrients: Beyond the Western prejudices
in Animal frontiers Volume 5 Issue 2, April 2015

Nutrient Requirements of Laboratory Animals,
Fourth Revised Edition, 1995 (1995)
Assessement methods and criteria
Oral exam
critical and in-depth analysis of a topic presented and discussed during the course
AGR/18 - ANIMAL NUTRITION AND FEEDING - University credits: 5
Practicals: 24 hours
Lessons: 18 hours