Molecular Virology

A.Y. 2019/2020
8
Max ECTS
66
Overall hours
SSD
VET/03 VET/05
Language
English
Learning objectives
Provide deep knowledge in the field of veterinary virology, driving the students to understand the mechanisms of virus replication, the interaction with cells in which they replicate, the diagnosis, the pathogenesis and the strategies to control viral diseases, including zoonosis. Laboratory practices aim to grow, isolate and characterize animal viruses, and to to identify and measure the abundance of Interferon Stimulated Genes in white blood cells infected by inactivated virus.
Expected learning outcomes
The student is expected to reach the following results:
     Theoretical learning:
basic veterinary molecular virology
background of the main viral diseases transmitted to human from animals, and molecular and biochemical changes related to these pathologies
main techniques applied in laboratories of virology and molecular diagnostics
    Practical learning:
application of in vitro methods to grow and isolate viruses
assessment of cytopathic effects due to different vituses on tissue culture
purification of viruses
application of molecular biology techniques to assess major biochemical and molecular modifications associated to viral diseases,
measurement of mRNA abundance expression and make judgements on its relationship with the effect of virus infection
assessment of the quality of a blood smear, and provide an interpretation of the results of a quantitative PCR base molecular assay.
    Communication:
understand and communicate using the proper terms related to Veterinary Virology and Viral Pathology  
Course syllabus and organization

Unique edition

Responsible
Lesson period
First semester
Course syllabus
{Modulo}: 'H52-48-A' - 'Unita' didattica: Molecular Basis of Virology '.
Tipo: 'Unità didattica'.
Theoretical classes:
Structure and composition of animal viruses
In vitro and in vivo characteristics of viral infections
Methods to study and to purify viruses
Diagnostic virology
How to grow viruses: animals, embryonated eggs and cell cultures
Anti-viral immune response
Anti-viral therapy
Viruses in gene therapy

Laboratory classes:
In vitro culture and splitting of a cell line
Cell count and procedure to freeze cell lines
In vitro infection
Cytopatic effects of different viruses on cell lines
Virus purification

{Modulo}: 'H52-48-B' - 'Unita' didattica: Molecular pathogenesis of viral diseases '.
Tipo: 'Unità didattica'.
Lectures:
1. Viral pathogenesis
2. Immune Response to Viral Infections
3. SARS Corona viruses
4. Filoviruses : Ebola and Marburg
5. HIV
6. Influenza viruses

Seminars:
1. Emerging viral diseases
2. Herpes viruses

Practices
- Infection of bovine monocytes with BVD virus. The students will carry out an experimental infection of bovine monocytes with the virus of BVD. Bovine will be purified and infected with viruses. Cytopathic effects will be identified, and the expression of proteins involved in immune reaction will be assessed
- Histopathology of viral infections
Prerequisites for admission
Not required any preliminary knowledge
Teaching methods
The teaching consists in theoretical classes and practical classes in laboratory
Bibliography
Handouts will be provided by the teacher through ARIEL website
Assessement methods and criteria
The obtainment of the expected learning outcomes by the student are assessed by a written examination based on questions with open answer and questions with closed answer, which include also the description of the practical activity.
The evaluation parameters include correctness of answer, comprehension of the topics of the program, ability to connect them and propriety of language.
The grade is expressed in thirtieths and it is communicated to the student by e-mail within a couple of days from the end of the written exam.
Unita' didattica: Molecular Basis of Virology
VET/03 - VETERINARY PATHOLOGY - University credits: 0
VET/05 - INFECTIOUS DISEASES OF DOMESTIC ANIMALS - University credits: 0
Practicals: 24 hours
Lessons: 18 hours
Professor: Turin Lauretta
Unita' didattica: Molecular pathogenesis of viral diseases
VET/03 - VETERINARY PATHOLOGY - University credits: 0
VET/05 - INFECTIOUS DISEASES OF DOMESTIC ANIMALS - University credits: 0
Practicals: 12 hours
Lessons: 12 hours
Professor: Ceciliani Fabrizio
Professor(s)
Reception:
Dipartimento di Scienze Veterinarie e Sanità Pubblica - Sezione Patologia Generale e Parassitologia