Omics

A.Y. 2020/2021
10
Max ECTS
66
Overall hours
SSD
BIO/10 BIO/18
Language
Italian
Learning objectives
The "Introduction to genomics" module presents to the students the main concepts dealing with genome organization. In facts a graded student in Veterinary Biotechnologies must have basic knowledges on the dimension and architecture of genome and chromosomes, in addition to the meaning of linkage and linkage disequilibrium between loci. Practical applications for animal breeding and veterinary medicine are also presented with the connected doctrinal and statistical tools, for example the use of bead DNA chip for genomic evaluation in cattle, parental exclusion or for general concepts and application in population genomics (F-statistics).
The Proteomic module presents essential information to study the complete proteome of a cell or tissue, its qualitatively and quantitative characterization and the analysis of possible modifications induced by a stimulus. Many different applications of this approach will be illustrated starting from published data.
Expected learning outcomes
Regarding the genomics module, at the end of the course the student will know the genome size, its organization in chromosomes, the meaning of linkage and Linkage Disequilibrium, the use of DNA bead chips for genomic evaluation in cattle or for ascertaining paternity as well as concepts of population genomics (F statistics).
As far as proteomics is concerned, the student will know the principles and applications of the main proteomic methodologies including; two-dimensional electrophoresis, mass spectrometry, MS and MS/MS, quantitative mass spectrometry, amino-terminal sequence, amino acid analysis and study of complexes. About the ability to do: he will be able to make and interpret a MALDI spectrum , an amino-terminal sequence and a 2D aleltrophoresis. He will also be able to read and discuss specific articles on proteomics
Course syllabus and organization

Unique edition

Responsible
Lesson period
First semester
Unita' didattica: Introduzione alla genomica
BIO/10 - BIOCHEMISTRY - University credits: 0
BIO/18 - GENETICS - University credits: 0
Lessons: 18 hours
Professor: Minozzi Giulietta
Unita' didattica: Proteomica
BIO/10 - BIOCHEMISTRY - University credits: 0
BIO/18 - GENETICS - University credits: 0
Practicals: 12 hours
Lessons: 36 hours
Professor: Tedeschi Gabriella