Environmental laboratory

A.Y. 2019/2020
Lesson for
18
Max ECTS
216
Overall hours
SSD
AGR/13 AGR/16 CHIM/06
Language
Italian
Learning objectives
The course "Environmental Laboratory" consists of three disciplines (environmental chemistry, environmental microbiology and environmental biochemistry and ecotoxicology) which include laboratory activities and lectures aimed at dealing with cases of contamination and environmental restoration. Research-oriented and problem-solving approaches are applied in the course. Activities of the three subjects are strongly integrated and teach students to work in group and to tackle problems with a multidisciplinary perspective and adequate critical evaluation approach.
The course will strength students on following topics:
- Distribution of organic and inorganic contaminations in different environmental compartments (water, air, soil)
- Analytical techniques for environmental monitoring
- Role of plants and microorganisms in environmental processes
- Biological approaches for environmental restoration, waste treatment, material recovery with reference to environmental legislation
- Statistical analysis of environmental data.
Students will acquire practical skills in the use of analytical instruments for the quantification of organic and inorganic compounds in the environment, microflora connected to environmental processes, physiological state of plants. In addition, soft skills like communication and discussion of scientific reports will be implemented.

Course structure and Syllabus

Active edition
Yes
Responsible
biochimica ambientale ed ecotossicologia
AGR/13 - AGRICULTURAL CHEMISTRY - University credits: 6
Laboratories: 48 hours
Lessons: 24 hours
environmental chemistry
CHIM/06 - ORGANIC CHEMISTRY - University credits: 6
Laboratories: 48 hours
Lessons: 24 hours
Environmental microbiology
AGR/16 - AGRICULTURAL MICROBIOLOGY - University credits: 6
Laboratories: 48 hours
Lessons: 24 hours
Professor: Cavalca Lucia
ATTENDING STUDENTS
environmental chemistry
Syllabus
Lectures (3 credits): Measure units. Heavy metals: chemistry and toxicology. Environmental sampling, samples storage. Spectroscopy: Electromagnetic radiation, Adsorption and emission spectroscopy: principles. Ultraviolet and Visible spectroscopy: Lambert Beer law, instrumentation and applications. Mass spectroscopy: theory, instrumentation, quadrupole detector, ICP-MS. Nuclear Magnetic Resonance (NMR): theory, instrumentation, chemical shift, spectra of elements other that H and C. Fundamentals of chromatography: retention time, selectivity, efficiency. Gas chromatography and HPLC, instrumentation, use in Workshop (3 credits): Safety rules in a chemical laboratory. Sampling on the site under investigation. Sample extraction, separation by TLC and HPLC and quantification by spectrophotometric methods of plant pigments. Analysis of heavy metals and other elements by means of ICP-MS. Recording NMR spectra of different nuclei.
Environmental microbiology
Syllabus
Lectures (3 credits): The microbiological sampling. Methods of analysis and monitoring of environmental microbial communities. Cell count methods on pollutants, enrichment cultures, microcosms. Environmental genomics: polymerase chain reaction (PCR), quantitative Real Time PCR, clone libraries, denaturing gradient gel electrophoresis (DGGE), Sanger sequencing, Illumina sequencing. Biodegradation of organic pollutants and genetics: monoaromatic hydrocarbons, polycyclic aromatic hydrocarbons (PAH), alifatics. Bacterial resistance and metabolism of heavy metals and Arsenic. Rhizosphere and endosphere microbiology: plant growth promoting bacteria, pollutants rhizo-degradation, metal translocation in plants (phytoremediation and food safety). Bioremediation of contaminated sites. Microbial life in extreme environments: the acid mine drainage. Microbial electrochemical systems: electroactive bacteria, microbial fuel cells, chemicals production from waste biomass. Seminars complete the preparation of the student: private bioremediation companies, public bodies involved in environmental monitoring and managing (ARPA, CNR, park authority).
Practical classes (3 credits): Safety in microbiology laboratories. Sampling on the site under investigation. Preparation and conservation of samples for microbiological and molecular analysis. Total aerobic, anaerobic bacterial plate count, MPN count of pollutant degrading bacteria. Phenotypic characterization of bacterial isolates: resistance to heavy metals and arsenic, mineralization of organic compounds, plant growth promotion (siderophores, IAA, EPS, phosphate solubilisation, ACC deaminase, mobility). DNA extraction from environmental samples. Preparation of polymerase chain reaction (PCR) and quantitative PCR: 16S rRNA and functional genes. Electrophoresis run on agarose gel. Analysis of nucleotide sequences. Data analysis and discussion. Writing the report.
NON-ATTENDING STUDENTS
Environmental microbiology
Syllabus
Lectures (3 credits): The microbiological sampling. Methods of analysis and monitoring of environmental microbial communities. Cell count methods on pollutants, enrichment cultures, microcosms. Environmental genomics: polymerase chain reaction (PCR), quantitative Real Time PCR, clone libraries, denaturing gradient gel electrophoresis (DGGE), Sanger sequencing, Illumina sequencing. Biodegradation of organic pollutants and genetics: monoaromatic hydrocarbons, polycyclic aromatic hydrocarbons (PAH), alifatics. Bacterial resistance and metabolism of heavy metals and Arsenic. Rhizosphere and endosphere microbiology: plant growth promoting bacteria, pollutants rhizo-degradation, metal translocation in plants (phytoremediation and food safety). Bioremediation of contaminated sites. Microbial life in extreme environments: the acid mine drainage. Microbial electrochemical systems: electroactive bacteria, microbial fuel cells, chemicals production from waste biomass.
Lesson period
First semester
Lesson period
First semester
Assessment methods
Esame
Assessment result
voto verbalizzato in trentesimi
Educational website(s)
Professor(s)
Reception:
By appointment
DEFENS-Environmental Microbiology, Via Mangiagalli 25, 3rd floor
Reception:
Every day (phone or e-mail appointment)
Office at Department of Agricultural and Environmental Sciences
Reception:
to take an appointment
office- DISAA
Reception:
Thursday 10:30 to 12:30 am
DISMA