General microbiology

A.Y. 2017/2018
Lesson for
5
Max ECTS
44
Overall hours
Language
Italian
Learning objectives
To acquire basic knowledge on the microbial world. The student should have the basic scientific knowledge to understand the microbial metabolism and to deal with the microbiological problems related to the food area in a critical way.
The student should be able to apply the knowledge of the General Microbiology course in a professional way. He must be able to apply a protocol for the isolation and the characterization of microbial strains using standard and innovative microbiological methods.

Course structure and Syllabus

Active edition
Yes
Responsible
Laboratories: 8 hours
Lessons: 36 hours
Professor: Mora Diego
Syllabus
Objective
The student should acquire a correct view of the
microbial world with a specific attention to the microbial
applications, quality and safety problems realted to the
food area.

Teaching program:
Microorganism definition. Description and use of the
phase-contrast microscope. The procaryotic and the
eucaryotic cell. Description of cell composition and
structures. Composition, structure and function of cell
membrane and cell wall. Description of teichoic-
lipoteichoic acids and exopolysaccharides. The
membrane transport systems. The chemical
composition of the cell and the nutritional
requirements. The composition of a microbiological
media: solid and liquid media. The sterilization
procedure. The microbial growth: the growth in
bacteria and in the yeast Saccharomyces cerevisiae.
Description of the growth curve. Description direct and
indirect methods for microbial count. The energetic
metabolism. The respiratory metabolism and electron
chain transport. Glycolysis, homo- and heterolactic
fermentation and lactic acid bacteria, the TCA cycle,
the ethanol fermentation of S. cerevisiae, the acetic
fermentation, the acetic metabolism and the acetic
bacteria. The anabolism and the definition of
auxotrophy and prototrophy. The anaplerotic reactions.
Principle of microbial genetics: the structure and
composition of DNA and RNA. The DNA replication. The
genetic code. The transcriptional gene organizations.
The RNA synthesis, the protein synthesis. Gene
regulation: example of positive and negative gene-
regulation. The mutations and the mechanisms of gene
repair. Plasmids and virus. The mechanisms of phage
resistance. The horizontal gene transfer mechanisms:
conjugation, transduction and transformation. Principle
of bacterial taxonomy: the species definition, the
polyphasic approach in the species identification. The
16S rRNA gene in the taxonomy of living organisms.
The PCR reaction: principle and applications.
Description of sporeforming bacteria and enteric
bacteria. Fungal taxonomy.

Practical laboratory exercises
During the microbiology laboratory lessons the student
will do practice in isolating pure bacterial culture from
sample of soil, yogurt and fermented meat. The
student will apply the viable count method to evaluate
the total microbial count in the above mentioned
samples. The student will learn to use a phase-contrast
microscope to observe fresh cell suspension of bacteria
and yeasts and to identify the Gram type of the strain
isolated in pure culture for the samples described
above. Moreover, the student will learn how to use
different media in order to test the amylolytic and
proteolytic activities of bacterial cultures.
Teaching methods
I lucidi delle lezioni e i libri di testo di riferimento sono
disponibili sul sito http://users.unimi.it/~dmora/WP/.
Il docente è disponibile per ulteriori chiarimenti su
appuntamento.
Lesson period
First semester
Lesson period
First semester
Assessment methods
Esame
Assessment result
voto verbalizzato in trentesimi
Professor(s)
Reception:
By e-mail appointment
Office, via Mangiagalli 25, third floor