biomolecular methods laboratory

A.Y. 2015/2016
9
Max ECTS
72
Overall hours
SSD
BIO/10 BIO/11 BIO/18
Language
English
Learning objectives
The course aims at the detailed understanding and knowledge of the molecular principles underlying the advanced techniques currently used in research laboratories.
Expected learning outcomes
Undefined
Course syllabus and organization

Single session

Responsible
Lesson period
year
Course syllabus
Classes held by the teachers are divided in three Units:
- Molecular Biology: Methodologies and techniques for heterologous expression in cellular and animal models.
Different methods to introduce exogenous DNA in cultured cells and different animal models will be discussed.
The use and the scope of the different methods will be also discussed.

- Genetics: DNA Sequencing and Next Generation Sequencing Technologies
The course is aimed to illustrate the main methods used for the extraction and purification of nucleic acids, including genomic DNA, plasmid DNA and RNA. Moreover, the principles and procedures of PCR, RT-PCR and qRT-PCR assays are explained.
The traditional methods of DNA sequencing, such as Sanger-Coulson and Maxam-Gilbert are discussed together with the Next-Generation sequencing approaches starting from the 454 strategy, going through the Solexa-Illumina and the recent Solid method.

-Biochemistry: Methodologies and techniques for heterologous expression and purification of recombinant proteins. Principles of protein purification and chromatography techniques. Bacterial expression systems and affinity chromatography. Case studies of multiprotein complexes purification strategies.

The course program also foresees a series of seminars and workshops, held by national and international speakers, that will illustrate how current and advanced techniques are exploited to answer the specific scientific questions, and how the scientific problems are approached by research laboratories active in different fields of Molecular and Cellular Biology.
At the end of the academic year, recent articles regarding the development and applications of advanced methodologies in research fields of interest will be presented and discussed by the students in a dedicated workshop.
Classes and seminars are held in english.


Reference Material
Slides and multimedia materials used for the classes will be made available on the dedicated Ariel website.

Prerequisites and examination procedures
Oral exam in English based on a written elaborate regarding the materials and methods used during the internship.


Recommended Prerequisites
All first year exams. Conclusion of the Internship research activity.

Teaching Methods
The course forsees 3 credits of classical format classes with lessons given by the teachers, and 6 credits of seminars and workshops.

Language
English


Program information
All the material used for the classes will be made available on the dedicated Ariel website.
Seminars and workshop dates will be published on the same Ariel site.

WEB Pages
ariel.unimi.it > Biomolecular Methods Laboratory
(http://ngnesuttambl.ariel.ctu.unimi.it)
BIO/10 - BIOCHEMISTRY - University credits: 0
BIO/11 - MOLECULAR BIOLOGY - University credits: 0
BIO/18 - GENETICS - University credits: 0
Lessons: 24 hours
Lessons (seminars): 48 hours
Professor(s)
Reception:
tuesday 11.30-12.30, by appointment via email
7C