Imaging in biomedical research

A.Y. 2015/2016
8
Max ECTS
48
Overall hours
SSD
BIO/10 VET/01
Language
English
Learning objectives
Undefined
Expected learning outcomes
Undefined
Course syllabus and organization

Single session

Responsible
Lesson period
Second semester
ATTENDING STUDENTS
Image analysis of cells and tissues
Course syllabus
Lectures
Basic concepts in imaging. Electromagnetic radiation and interactions with Matter (2 hours),
Optics, including lenses and reflection. Generators, filters and detectors (2 hours)
Biological effects of radiation. Safety considerations (2 hours).
Imaging systems for biological molecule (Proteins, lipids, nucleic acids) (2 hours)
Transmission scanning microscopy (2 hours)
Fluorescence microscopy (2 hours)

Practical training
Time-lapse of living systems (2 hours)
Use of algorithms used in imaging (2 hours)
Processing, digitalization deconvolution (2 hours)
Image degradation, Spatial resolution, Signal and noise (2 hours)
Image measurements, histograms (2 hours)
Use of Image 1.62 NIH program (2 hours)
Mass spectrometry as imaging tool
Course syllabus
The course is aimed at providing information concerning the application of MALDI as an imaging technique.
Lectures:
Sample preparation
MALDI imaging
Proteins identification and analysis
Molecular Imaging
Course syllabus
Lectures
Confocal and multiphoton microscopy (2 hours)
Imaging of live and fixed cells (2 hours)
Ultrastructural analysis (1 hours)
Fluorescence Resonance Energy Transfer (1 hours)
Use of radioisotopes for imaging con radionuclide (1 hour)
Magnetic resonance imaging (1 hour)
Computerized Tomography (1 hours)

Practical training
Use of Leica Confocal System (1 hours)
Use of Nikon NKM Program (2 hours)
NON-ATTENDING STUDENTS
Image analysis of cells and tissues
Course syllabus
Lectures
Basic concepts in imaging. Electromagnetic radiation and interactions with Matter (2 hours),
Optics, including lenses and reflection. Generators, filters and detectors (2 hours)
Biological effects of radiation. Safety considerations (2 hours).
Imaging systems for biological molecule (Proteins, lipids, nucleic acids) (2 hours)
Transmission scanning microscopy (2 hours)
Fluorescence microscopy (2 hours)

Practical training
Time-lapse of living systems (2 hours)
Use of algorithms used in imaging (2 hours)
Processing, digitalization deconvolution (2 hours)
Image degradation, Spatial resolution, Signal and noise (2 hours)
Image measurements, histograms (2 hours)
Use of Image 1.62 NIH program (2 hours)
Mass spectrometry as imaging tool
Course syllabus
The course is aimed at providing information concerning the application of MALDI as an imaging technique.
Lectures:
Sample preparation
MALDI imaging
Proteins identification and analysis
Molecular Imaging
Course syllabus
Lectures
Confocal and multiphoton microscopy (2 hours)
Imaging of live and fixed cells (2 hours)
Ultrastructural analysis (1 hours)
Fluorescence Resonance Energy Transfer (1 hours)
Use of radioisotopes for imaging con radionuclide (1 hour)
Magnetic resonance imaging (1 hour)
Computerized Tomography (1 hours)

Practical training
Use of Leica Confocal System (1 hours)
Use of Nikon NKM Program (2 hours)
Image analysis of cells and tissues
VET/01 - VETERINARY ANATOMY - University credits: 4
Lessons: 24 hours
Professor: Brevini Tiziana
Mass spectrometry as imaging tool
BIO/10 - BIOCHEMISTRY - University credits: 2
Lessons: 12 hours
Professor: Tedeschi Gabriella
Molecular Imaging
VET/01 - VETERINARY ANATOMY - University credits: 2
Lessons: 12 hours
Professor: Brevini Tiziana