This course aims at presenting the major genetic and molecule biological experimental strategies for studying gene and protein function, and for the characterization of pathways. Genome-wide strategies for functional genomics will also be described.
Expected learning outcomes
Lesson period: First semester
(In case of multiple editions, please check the period, as it may vary)
Genomic and cDNA libraries Screening approaches basedon sequence, strucutre and function Analysis of gene expression: microarrays and RNA seq Studying the function of genes: knock-in, knock-out, RNA interference, site specific mutagenesis In vitro expression cloning Synthetic Genomic Array analysis Functional analysis through the study of genetic and physical interactions Protein tagging Approaches for proteome analysis