Principles of functional genomics

A.Y. 2015/2016
6
Max ECTS
48
Overall hours
SSD
BIO/18
Language
Italian
Learning objectives
This course aims at presenting the major genetic and molecule biological experimental strategies for studying gene and protein function, and for the characterization of pathways. Genome-wide strategies for functional genomics will also be described.
Expected learning outcomes
Undefined
Course syllabus and organization

Single session

Responsible
Lesson period
First semester
Course syllabus
Genomic and cDNA libraries
Screening approaches basedon sequence, strucutre and function
Analysis of gene expression: microarrays and RNA seq
Studying the function of genes: knock-in, knock-out, RNA interference, site specific mutagenesis
In vitro expression cloning
Synthetic Genomic Array analysis
Functional analysis through the study of genetic and physical interactions
Protein tagging
Approaches for proteome analysis
BIO/18 - GENETICS - University credits: 6
Lessons: 48 hours
Professor: Muzi Falconi Marco
Professor(s)