Principles of functional genomics
A.Y. 2015/2016
Learning objectives
This course aims at presenting the major genetic and molecule biological experimental strategies for studying gene and protein function, and for the characterization of pathways. Genome-wide strategies for functional genomics will also be described.
Expected learning outcomes
Undefined
Lesson period: First semester (In case of multiple editions, please check the period, as it may vary)
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Course syllabus and organization
Single session
Responsible
Lesson period
First semester
Course syllabus
Genomic and cDNA libraries
Screening approaches basedon sequence, strucutre and function
Analysis of gene expression: microarrays and RNA seq
Studying the function of genes: knock-in, knock-out, RNA interference, site specific mutagenesis
In vitro expression cloning
Synthetic Genomic Array analysis
Functional analysis through the study of genetic and physical interactions
Protein tagging
Approaches for proteome analysis
Screening approaches basedon sequence, strucutre and function
Analysis of gene expression: microarrays and RNA seq
Studying the function of genes: knock-in, knock-out, RNA interference, site specific mutagenesis
In vitro expression cloning
Synthetic Genomic Array analysis
Functional analysis through the study of genetic and physical interactions
Protein tagging
Approaches for proteome analysis
Professor(s)