Applications of biotech on medicine

A.Y. 2016/2017
Overall hours
MED/08 MED/40 MED/42 MED/43 MED/44 MED/46
Learning objectives
The aim of the course is to provide students with a basic theoretical
understanding of some fields of biotechnological applications in medicine, namely
vaccine technology, epigenetics applied to occupational medicine, reproductive medicine,
forensic medicine, cell culture and stem cell technologies.
Expected learning outcomes
Understanding and analysis of basic biological phenomena and methodologic approaches to their study in relation to current or possible biomedical applications
Course syllabus and organization

Single session

Lesson period
Second semester
Course syllabus
Biotechnology of reproduction
Ovarian and endometrial cycle- spermatogenesis- conception
Implantation, develpment and function of the placenta - Fetal growth - Main embryo and trophoblast pathologies-pregnancy related diseases
Causes of infertility and clinical indications to assisted reproduction - in vitro assisted reproduction techniques
Crioconservation of gametes and reproductive tissues - stem cells for reproductive purposes - preimplantation diagnosis - Genomic imprinting
Invasive and non-invasive prenatal diagnosis techniques - reproductive techniques in couples with blood viral infections

Biotechnologies and public health.
- General principles. Hygiene and public health. Evolution of the "health" concept. Health and disease.
- Epidemiology methodology. Definition and objectives. Epidemiological studies. The molecular epidemiology approach..
- Prevention methodology. Definition and objectives. Molecular biology in the prevention field.
Vaccines and vaccination strategies.
- Biotechnologies and development of new generation vaccines.
Public health topicality.
- Emerging and re-emerging infectious diseases.
- New technologies as tools to face up to new health emergences.

Forensic Science and the role of experts in the Italian Legal system.
Basics of admissibility standards of expert evidence in the Italian and North American legal systems.
Introduction to Forensic genetics. The medico-legal approach to biological evidence: presumptive and confirmatory tests and screening for biological components, species, sex and personal DNA identification.
The collection and storage of biological evidence. The chain of custody.
Molecular basis of human DNA identification: length and sequence polymorphisms. Autosomal and lineage markers (Y chromosome and Mitochondrial DNA polymorphisms).
The process of forensic validation.
Introduction to the interpretation of evidence based on DNA profiling and in paternity testing.
Future perspectives in forensic DNA analyses.

Introduction and General Concepts.
Technologies for DNA Methylation and Histone Modification Analyses.
Epigenetics: Environmental Instructions for the Genome.
Design of Epidemiology Studies and Epigenetic Aging
Health Correlates of Epigenetic Alterations. Genomic Imprinting and Epigenetic Inheritance.
Diet and Epigenetic States. Epigenetics and Cancer.
Epigenetics Alterations of Cardiovascular, Respiratory and Neurological Diseases.
Non-coding RNAs.

Cell and tissue culture methods, laboratory and requirements.
Tissue and cells bank, cryo- preservation
Cell culture in biomedical diagnosis ( amniocytes culture, fibroblasts culture)
Cell culture in biomedical research
tumor invasion and metastasis
signal transduction
Hematopoietic cells
Tumor stem cell
Cell migration: molecular mechanisms and in vitro models
Cell death: methods to study apoptosis and necrosis
Integrins extracellular matrix, growth factors: characteristic and dynamic changes
during tumour progression; and targets for new cancer therapeutics.
MED/08 - PATHOLOGY - University credits: 0
MED/40 - OBSTETRICS AND GYNAECOLOGY - University credits: 0
MED/42 - HYGIENE AND PUBLIC HEALTH - University credits: 0
MED/43 - FORENSIC MEDICINE - University credits: 0
MED/44 - OCCUPATIONAL MEDICINE - University credits: 0
Lessons: 56 hours
On Appointment
Istituto Clinico Humanitas, Via A. ;anzoni 113, Rozzano, Milano
via C. Pascal, 36 Milan
Monday 12.00-13.00
Interhospital Pathology Division, Science and Technology Park, Via Fantoli 16/15, 20138 Milano