The aim of the course is to provide students with all the knowledge needed to master the basics of biological chemistry, particularly with regard to the understanding and study of the major biological processes in living organisms.
Structure, chemical properties and reactivity of carbohydrates (mono-, di- and polysaccharides).
Nucleic acids: bases, nucleosides, nucleotides, base-pairing, DNA structure
Structure, chemical properties and reactivity of amino acids. Chemical synthesis of peptides (in solution and in solid phase), protective groups.
Protein structure and folding
Oxygen transport proteins: myoglobin and haemoglobin.
Enzymes: basis concepts and kinetics, cofactors and coenzymes, the Michaelis-Menten equation, regulation of the enzymatic activity
Enzymatic inhibition: reversible (competitive, incompetitive and non-competitive) and irreversible inhibitors
Serine proteases: general properties, three dimensional structure, activation and catalytic mechanism.
Bioenergetics: high-energy compounds. ATP and other phosphorylated compounds. Redox reactions. Electron carriers: NAD+ and FAD
Metabolism: basic concepts and regulation, glycolysis, acetil-CoA synthesis, citric acid cycle, electron transport and oxidative phosphorylation
Mandatory Organic Chemistry I
- Voet, Voet, Pratt: Principles of Biochemistry, Wiley
- Garrett, Grisham: Principles of Biochemistry, Cengage Learning.
- Nelson, Cox: Lehninger Principles of Biochemistry, W H Freeman & Co
- Petsko, Ringe, Protein Structure and Function, Blackwell Publishing Ltd
Copies of the slides projected in the classroom as well as other materials will be made available through the course website on the ARIEL platform of the University of Milano (http://ariel.ctu.unimi.it
). By no means this material replaces the lectures or a textbook. The material is made available only to registered students of the Degree Course in Biological Chemistry and should not be distributed to others.
The exam consists of a written test which aims to evaluate the student's knowledge on the theoretical and practical aspects of the subject treated in the course. The written test requires open answers to questions and/or the solution of exercises. During the examination it is not allowed to consult texts or notes.
Language of instruction
Mode of teaching: