Methodologies of Genetics and Plant Biotechnology are an essential tool to improve genetically crop species. This course is aimed to provide the necessary information to understand the molecular mechanisms at the basis of the strategies employed to generate genetically modified organisms.
Expected learning outcomes
Lesson period: First semester
(In case of multiple editions, please check the period, as it may vary)
The course has the aim to provide the technical and theoretical information to address topics related to the genetic improvement of plant species based on molecular biology and genetic engineering. The first part of the course will be focused on the realization of the constructs necessary for the generation of transgenic plants. In particular, the essential elements of cloning and expression vectors, such as Promoters, Enhancers, Multi-cloning Sites, Gateway Gene Cloning, Terminators, Marker and Reporter genes will be described Within the second part of the course, the strategies used to analyze transgenic plants will be illustrated. In particular, the approaches used to identify the insertion site(s) of the transgene and its expression will be discussed. Moreover, epigenetic aspects related to gene silencing and possible alternatives to prevent silencing of the transgene will be also explained. The third part of the course will be dedicated to GMO organisms currently on the market, including plants resistant to herbicides and pathogens or used as bioreactors for the production of antibodies, vaccines and other pharmaceutical products. Within this context, the transplastomic approach is taken into consideration.
Supporting Materials The supporting materials including scientific papers and slides will be provided during the course
Procedure of examination The oral examination is aimed to verify that the student has gained knowledge on the different topics discussed during the course.
Prerequisites It is recommended that students have basic knowledge on Molecular Biology and Genetics
Teaching methodology Classical format classes in Italian