Methods in plant genetics and biotechnology

A.Y. 2016/2017
Overall hours
Learning objectives
Methodologies of Genetics and Plant Biotechnology are an essential tool to improve genetically crop species. This course is aimed to provide the necessary information to understand the molecular mechanisms at the basis of the strategies employed to generate genetically modified organisms.
Expected learning outcomes
Course syllabus and organization

Single session

Lesson period
First semester
Course syllabus
The course has the aim to provide the technical and theoretical information to address topics related to the genetic improvement of plant species based on molecular biology and genetic engineering. The first part of the course will be focused on the realization of the constructs necessary for the generation of transgenic plants. In particular, the essential elements of cloning and expression vectors, such as Promoters, Enhancers, Multi-cloning Sites, Gateway Gene Cloning, Terminators, Marker and Reporter genes will be described
Within the second part of the course, the strategies used to analyze transgenic plants will be illustrated. In particular, the approaches used to identify the insertion site(s) of the transgene and its expression will be discussed. Moreover, epigenetic aspects related to gene silencing and possible alternatives to prevent silencing of the transgene will be also explained.
The third part of the course will be dedicated to GMO organisms currently on the market, including plants resistant to herbicides and pathogens or used as bioreactors for the production of antibodies, vaccines and other pharmaceutical products. Within this context, the transplastomic approach is taken into consideration.

Supporting Materials
The supporting materials including scientific papers and slides will be provided during the course

Procedure of examination
The oral examination is aimed to verify that the student has gained knowledge on the different topics discussed during the course.

It is recommended that students have basic knowledge on Molecular Biology and Genetics

Teaching methodology
Classical format classes in Italian
BIO/18 - GENETICS - University credits: 6
Lessons: 48 hours
Professor: Pesaresi Paolo