The first part of the course provides a detailed overview of the molecular mechanisms of cell cycle control. The second part will focus on the structure of the genome and the molecular mechanisms that regulate genome stability. Human diseases resulting from defects in the mechanisms of cell cycle control and in processes responsible for maintaining the integrity of the genome will be taken into consideration.
The eukaryotic Cell Cycle: - Model organisms in the cell cycle analysis; - The cell cycle control systems; - The control of S phase; - Early mitosis: preparing the chromosomes to segregation; - Assembly of the mitotic spindle; - The completion of mitosis and the cytokinesis; - The control of meiosis; - Control of cell proliferation and growth; - The checkpoints of the cell cycle in physiological and pathological growth conditions; - Alterations of cell cycle in cancer and other human diseases.
The maintenance of genome stability: - The eukaryotic chromosomes: structure and organization, centromeres and telomeres; - Alteration of the structure and number of chromosomes; - Different types of DNA damages; - Essential molecular mechanisms that preserve the correct structure of chromosomes; - A plethora of DNA repair pathways; - DNA damage checkpoints that control genomic stability; - Human disorders whose cause is a defect in the response to or repair of DNA damage; - Molecular mechanisms responsible for the development of the immune system; - The molecular pathways involved in the maintenance of genomic stability as targets of therapeutic treatments.
D.O. Morgan. The Cell Cycle: principles of control. New Science Press, 2007. J.D. Watson, T.A Baker, S.P. Bell, A. Gann, M. Levine and R. Losick "Molecular Biology of the Gene" VI Edition. Friedberg, E. C., Walker, G. C., Siede, W., Wood, R. D., Schultz, R. A. & Ellenberger, T. (2005) DNA repair and mutagenesis (ASM Press). Research and review articles concerning the topics dealt with during lectures will be suggested.
Prerequisites and examination procedures
Good knowledge of Molecular Biology is required. Written examination (open questions); students who attend classes regularly can take the exam passing a written test in progress and at the end of the course.
Teaching Mode: Traditional. Attendance: highly recommended. Language of instruction Italian
The lessons in pdf format will be available on the ARIEL website of the teacher.