In Vitro Model Technologies

A.Y. 2016/2017
5
Max ECTS
54
Overall hours
SSD
VET/02 VET/07
Language
Italian
Learning objectives
Undefined
Expected learning outcomes
Undefined
Course syllabus and organization

Single session

Responsible
Lesson period
First semester
ATTENDING STUDENTS
Unita' didattica: Tossicologia e modelli in vitro
Course syllabus
lectures
Introduction to in vitro Toxicology in vitro (1 hour)
In vitro models and evaluation of bioavailability, transport, metabolism and toxicity (4 hour)
Nanotoxicology: in vitro toxicity of nanoparticles (1 hour)
Practical
Cytotoxicity assays: release of LDH, MTT test, Neutral Red Up-take (6 hours)
Evaluation of the release of interleukin 1 alpha (2 hours)
Evaluation of the release of TNF-alpha (2 hours)
Toxicity studies in vitro with 3D models (4 hours)
Epithelial barriers and in vitro permeability studies (4 hours)
Evaluation of TEER (Trans-Epithelial Electric Resistance) (2 hours)
Endocrine Disruptor and in vitro models (2 hours)
In vitro hepatotoxicity and metabolism (2 hours)
Unita' didattica: Fisiologia e veicolazione di sistemi cellulari/tessutali
Course syllabus
The course will provide a part of the educational scientific basis for setting up systems of selection, storage, conveyance and release of cell populations, autologous and heterologous mammalian appropriate animal organs or body structures, other functional elements for create neoorgani and pseudorgani aimed at scientific research and biotechnology "in vitro", aimed at studying the regulation and modulation of various biological activities. Through these models of organs, it will be possible to arrive at an objective assessment of any potential use "in vivo".
The program will be as follows:
Preparation and Treatment of samples
Determination of the biological activity of sex hormones
Characterization of secreted through laboratory testing
NON-ATTENDING STUDENTS
Unita' didattica: Tossicologia e modelli in vitro
Course syllabus
lectures
Introduction to in vitro Toxicology in vitro (1 hour)
In vitro models and evaluation of bioavailability, transport, metabolism and toxicity (4 hour)
Nanotoxicology: in vitro toxicity of nanoparticles (1 hour)
Practical
Cytotoxicity assays: release of LDH, MTT test, Neutral Red Up-take (6 hours)
Evaluation of the release of interleukin 1 alpha (2 hours)
Evaluation of the release of TNF-alpha (2 hours)
Toxicity studies in vitro with 3D models (4 hours)
Epithelial barriers and in vitro permeability studies (4 hours)
Evaluation of TEER (Trans-Epithelial Electric Resistance) (2 hours)
Endocrine Disruptor and in vitro models (2 hours)
In vitro hepatotoxicity and metabolism (2 hours)
Unita' didattica: Fisiologia e veicolazione di sistemi cellulari/tessutali
Course syllabus
The course will provide a part of the educational scientific basis for setting up systems of selection, storage, conveyance and release of cell populations, autologous and heterologous mammalian appropriate animal organs or body structures, other functional elements for create neoorgani and pseudorgani aimed at scientific research and biotechnology "in vitro", aimed at studying the regulation and modulation of various biological activities. Through these models of organs, it will be possible to arrive at an objective assessment of any potential use "in vivo".
The program will be as follows:
Preparation and Treatment of samples
Determination of the biological activity of sex hormones
Characterization of secreted through laboratory testing
Unita' didattica: Fisiologia e veicolazione di sistemi cellulari/tessutali
VET/02 - VETERINARY PHYSIOLOGY - University credits: 0
VET/07 - VETERINARY PHARMACOLOGY AND TOXICOLOGY - University credits: 0
Practicals: 24 hours
Professor: Vigo Daniele
Unita' didattica: Tossicologia e modelli in vitro
VET/02 - VETERINARY PHYSIOLOGY - University credits: 0
VET/07 - VETERINARY PHARMACOLOGY AND TOXICOLOGY - University credits: 0
Practicals: 24 hours
Lessons: 6 hours
Professor: Caloni Francesca
Professor(s)
Reception:
Appointment by email
Office