Biochemistry and Molecular Biology

A.Y. 2017/2018
Overall hours
Learning objectives
Gli obiettivi del corso sono: (1) fornire le conoscenze necessarie alla comprensione della struttura e della funzione delle principali biomolecole costituenti gli organismi viventi; (2) descrivere i principali processi biologici presenti nelle cellule animali, con particolare riferimento a quelli coinvolti nel metabolismo delle molecole di origine alimentare; (3) fornire conoscenze necessarie alla comprensione del ruolo e della funzione delle macromolecole informazionali e delle principali metodologie di base inerenti la tecnologia del DNA ricombinante; (4) fornire le nozioni teorico-pratiche necessarie per la esecuzione pratica di semplici saggi colorimetrici.
Expected learning outcomes
Course syllabus and organization

Single session

Lesson period
Second semester
Modulo: Propedeutica biochimica
Course syllabus
Theoretical teaching (16 hours)
Organic nomenclature and main organic chemistry reactions of biological relevance of the following compounds. Hydrocarbons, alcohols and tioalcohols, amines, ethers, aldehydes, ketones, hemiacetal and acetals. Carboxylic acids and their derivatives. Phosphoesters and mixed phosphoanhydrides 4 h

Sterechemistry: conformations, geometric stereoisomers ( cis/trans), optical stereoisomers ( D/L) 2 h
Propedeutical Biochemistry: Carbohydrates. Aldoses and ketoses. D and L series. Monosaccharides: Haworth and conformational forms. Anomers and epimers. O- and N- glycosidic bonds. Reducing and non reducing disaccharides. Polysaccharides 3 hours
Propedeutical Biochemistry: Amino acids and Proteins. Proteic and non-proteic amino acids. Properties of free amino acids. Classification of proteic amino acids based on chemical properties of side chains. Essential and non-essential amino acids. Peptide bond, peptides and poly-peptides. Proteins. Amino acid composition of proteins and complex biological matrices including food 3 h

Propedeutical Biochemistry: Lipids. Fatty acids. Omega-9, -6, -3 unsaturated fatty acids. Triacylglycerols. Phospholipids. Cholesterol. Idrophobicity, amphypatic molecules, tensioactives 2 h

Propedeutical Biochemistry: Nuicleotides. Purinic and pyrimidinic bases, nucleosides and nucleotides. Polynucleotides. Non-nucleic acid constituing nucleotides 2 h

Practical training and seminars (16 hours)
Exercises on organic nomenclature and mai organic reaction of biochemical relevance 6 h
Exercises on the assignment of oxidation number of C atoms in organic compounds and redox reactions 2 h
Stereochemistry of organic compounds using molecular models 1 h
Bioenergetics of biological relevant reactions: seminar with numerical examples 3 h
Seminar: primary, secondary, tertiary and quaternary structure of proteins and their effects on biological activity 4 h
Modulo: Biochimica strutturale
Course syllabus
Structure and function of membranes. Transport across membranes 3 h
Collagen 1 h
Oxygen-transporting proteins: myoglobin and hemoglobin 3 h
Lipoproteins, fatty acid binding proteins 1 h
Enzymes and co-enzymes 1 h
Molecular mechanisms of digestion, absorption and transport of the main nutritionally relevant classes of compounds 4 h
Structure and function of nucleic acids 2 h
DNA replication 2 h
Transcription 2 h
Genetic code, translation, post-translational modifications 3 h
Overview of molecular mechanisms of hormone action and signal transduction 2 h
Modulo: Biochimica metabolica
Course syllabus
Theoretical lectures (16 h)
Glycolysis, pentose phosphate pathway, gluoconeogenesis, biosynthesis of lactose 4 h
Metabolism of glycogen 2 h
Metabolism of fatty acids, ketone bodies, Overview of metabolism of triacylglycerols, phosphoglycerids, sfingolipids and cholesterol and steroids 4 h
Krebs cycle and oxidative phosphorylation 3 h
Metabolism of amino acids: amino acid degradation and the urea cycle, overview of amino acid biosynthesis of nucleotide metabolism 3 h

Practical laboratories and seminar (16 h)
Seminar on anaerobic metabolism, fermentation and rumen metabolism 1 h
Seminar: mechanisms of enzyme regulation. Zymogens 3 h
Theory of spectrophotometrical determination of biological compounds 1 h
Colorimetrical determination of glucose and alkaline phosphatase 3 h
Colorimetrical of the protein content of a sample; lipase catalyzed digestion of milk lipids 3 h
Seminar: molecular basis of oxidative stress 1 h
Seminar on molecular biology applicative aspects: recombinant proteins and Polymerase chain reaction 2 h
Seminar on gene expression mechanisms 1 h
Proteomics 1 h
Modulo: Biochimica metabolica
BIO/10 - BIOCHEMISTRY - University credits: 3
Practicals: 16 hours
Lessons: 16 hours
Professor: Negri Armando
Professor: Negri Armando
Attività esercitativa ripetute a gruppi di studenti
Professor: Negri Armando
Modulo: Biochimica strutturale
BIO/10 - BIOCHEMISTRY - University credits: 3
Lessons: 24 hours
Professor: Negri Armando
Modulo: Propedeutica biochimica
BIO/10 - BIOCHEMISTRY - University credits: 3
Practicals: 16 hours
Lessons: 16 hours
Professor: Negri Armando