Microbiology and Hygiene

A.Y. 2018/2019
8
Max ECTS
88
Overall hours
SSD
BIO/19
Language
Italian
Learning objectives
Istruire gli studenti sulle caratteristiche generali dei microrganismi, sulle loro attività biologiche e patogene, sui concetti basilari dell'igiene, con particolare attenzione all'epidemiologia e profilassi delle malattie infettive.
Expected learning outcomes
Undefined
Course syllabus and organization

Single session

Responsible
Lesson period
Second semester
ATTENDING STUDENTS
Course syllabus
Microbiology and Hygiene

Objectives
The aim of the course on Applied Microbiology PhD Program is to teach the students of the Pharmacy PhD Program on general characteristics of fungi, bacteria, viruses and prions and in particular, on their biology and pathology. Detailed informations on Epidemiology and Prophylaxis of Infectious Diseases will also part of the course as well as the utilization of new molecular probes for the identification of pathogenic microbial agents.

Program

1. General Microbiology
General characteristics of microorganisms. Classification of living organisms, Archaea, Bacteria, Eucarya

2. Morphology and Structure of Bacteria
Morphological analysis: Gram and Ziehl-Neelsen stainings; microscopic examination of bacteria by optical and electron microscopy.
Structural components: capsule, flagellum, pilum, cell wall, cell membrane, cytoplasm, inclusion bodies, endospore.
Chromosomal and extra-chromosomal material. Genetic variability: mutations, and genetic transfer by cell transformation, conjugation and transduction

3. Functions and Activities of Bacteria
Nutrition, metabolism, respiration, fermentation, growth and multiplication. Bacterial cell culture. Liquid and agar media, chemical and physical parameters.

4. Viruses
Viral components. Replication of DNA and RNA viruses. Pathogenic activity of viruses: cytopathic and transforming viruses. Isolation, identification and culture of viruses. Cell cultures.

5. Hygiene
Hygiene and health definitions. Basic knowledge about demography and epidemiology.
General and specific prophylaxis, Sterilization and disinfection.
Pathogenic activity: microorganism virulence. Host defenses. The immune system.
Microorganism spreading inside the population

Epidemiology and Prophylaxis of Infectious Diseases
Air transmitted diseases: rubella, scarlet fever, smallpox, german measles, mumps, influenza, meningitis, pertussis, diphtheria, tuberculosis
Sexually transmitted diseases: syphilis, gonorrhea, clamidia, AIDS
Oro-foecal transmitted diseases: typhus-paratyphoid fever, cholera, salmonella, poliovirus
Vector transmitted diseases: malaria, yellow fever, plague
Other bacterial or viral transmitted diseases: anthrax, tetanus, rabies

Laboratory experiments:
Microbiological food (milk) examination
Microbiological water (river, lake) examination
Bacterial cell culture quantification
Antibacterial activity of antibiotics and disinfectants
Microbiological antibiotics titration
Plasmid extraction from E. coli cells
Gram staining
NON-ATTENDING STUDENTS
Course syllabus
Microbiology and Hygiene

Objectives
The aim of the course on Applied Microbiology PhD Program is to teach the students of the Pharmacy PhD Program on general characteristics of fungi, bacteria, viruses and prions and in particular, on their biology and pathology. Detailed informations on Epidemiology and Prophylaxis of Infectious Diseases will also part of the course as well as the utilization of new molecular probes for the identification of pathogenic microbial agents.

Program

1. General Microbiology
Characteristics of microorganisms. Comparison between eucaryotic and procaryotic cells, viruses and prions. Intermediate forms: mycoplasms, rickettsiae, chlamydias. Classification of living organisms, Archaea, Bacteria, Eucarya.

2. Morphology and Structure of Bacteria
Morphological analysis: Gram and Ziehl-Neelsen stainings; microscopic examination of bacteria by optical and electron microscopy.
Structural components: capsule, flagellum, pilum, cell wall, cell membrane, cytoplasm, inclusion bodies, endospore.
Chromosomal and extra-chromosomal material. Genetic variability: mutations, and genetic transfer by cell transformation, conjugation and transduction

3. Functions and Activities of Bacteria
Nutrition, metabolism, respiration, fermentation, growth and multiplication. Bacterial cell culture. Liquid and agar media, chemical and physical parameters.

4. Viruses
Viral components. Replication of DNA and RNA viruses. Pathogenic activity of viruses: cytopathic and transforming viruses. Isolation, identification and culture of viruses. Cell cultures.

5. Hygiene
Hygiene and health definitions. Basic knowledge about demography and epidemiology.
General and specific prophylaxis, Sterilization and disinfection.
Pathogenic activity: microorganism virulence. Host defenses. The immune system.
Microorganism spreading inside the population

Epidemiology and Prophylaxis of Infectious Diseases
Air transmitted diseases: rubella, scarlet fever, smallpox, german measles, mumps, influenza, meningitis, pertussis, diphtheria, tuberculosis
Sexually transmitted diseases: syphilis, gonorrhea, clamidia, AIDS
Oro-foecal transmitted diseases: typhus-paratyphoid fever, cholera, salmonella, poliovirus
Vector transmitted diseases: malaria, yellow fever, plague
Other bacterial or viral transmitted diseases: anthrax, tetanus, rabies.
BIO/19 - MICROBIOLOGY - University credits: 8
Single bench laboratory practical: 48 hours
Lessons: 40 hours
Professor: Zanotto Carlo
Professor(s)