Experimental medicine

Dottorati
Doctoral programme (PhD)
A.Y. 2021/2022
Study area
Medicine and Healthcare
Doctoral programme (PhD)
3
Years
Dipartimento di Biotecnologie mediche e medicina traslazionale - Via L. Vanvitelli, 32 - 20129 Milano
English
PhD Coordinator
Experimental medicine develops and applies technology and biological models for studying pathogenic mechanisms in human disease. It involves various disciplines (biochemistry, biology, molecular biology, anatomy, physiology, pathology, immunology, genetics and microbiology/virology) that are providing a rapidly growing understanding of the mechanisms that enable the development of pathological processes. The course aims to acquaint students with the technologies and research methodologies in the area of biomedicine and biotechnology based on in vitro and in vivo experimental models and '-omic' approaches, and on their application to the study of cellular and molecular mechanisms involved in the pathogenesis of human disease. The final objective is the formation of high-level scientists able to combine an understanding of biological processes and pathogenic mechanisms through the application of advanced experimental techniques. The programme's specific objectives are:
1) develop the ability to define problems and devise experiments to solve them in accordance with scientific standards;
2) study biochemical, molecular and cellular mechanisms in physiopathology using advanced technologies;
3) develop the ability to critically assess scientific literature;
4) provide solid, up-to-date knowledge in the specific field;
5) teach how to conduct independent research, and inform on related ethical implications.
Tutte le classi di laurea magistrale - All classes of master's degree
Dipartimento di Biotecnologie mediche e medicina traslazionale - Via L. Vanvitelli, 32 - 20129 Milano
Title Professor(s)
Exploring the link between circulating factors released in fluids of patient with rheumatoid arthritis and the increased susceptibility to psychiatric diseases
Investigation of the molecular regulatory networks that orchestrate age-associated processes combining biological and computational integrative approaches
Requirements: Knowledge of molecular biology methods, biochemistry, omics technologies, cell culture, data analysis, bioinformatics
The role of LSD1 in the modulation of glutamatergic neuroplasticity and its implications in neurodevelopmental and neuropsychiatric disorders
Requirements: Experience in cellular and molecular biology along with some experience of in vivo experiments
Brain stroma as an overlooked pharmacological target for Globoid Cell Leukodystrophy
Requirements: Strong commitment to basic and translational researc, well-developed social skills, a hands-on attitude and team-work orientation
Combined use of next-generation sequencing, in vitro and in vivo models to uncover the complex genetic basis of Congenital Hypogonadotropic Hypogonadism (CHH)
Requirements: Eventual experience on endocrine regulatory mechanisms of reproduction, whole exome sequencing technique and analysis, molecular biology techniques for gene expression studies, zebrafish model and pluripotent stem cells
New mechanisms controlling macrophages functions in atherosclerosis
Requirements: Basic cellular and molecular biology; biochemical competences
Investigating Connexin 43 role and tuning in osteoarthitis
Requirements: Strong scientific motivation. Any experience in cell culture and molecular biology is appreciated. Strong commitment to basic and translational research are welcome
Minibrains and Inflammation
Requirements: Cell culture, Western Blotting, Nucleic acids extraction, RealTime-PCR, gene silencing and gene editing.
The role of Notch pathway in the pathological communication mediated by extracellular vesicles- in multiple myeloma microenvironment
Requirements: Technical skills in molecular and cellular biology, team spirit and flexibility, high motivation. A good background on cancer development and progression, tumor microenvironment and immune system, experience in flow cytometry and omics data analysis are appreciated.
Impact of Drp1 activation, Unfolded Protein Response induction and FGF21 levels in DMD progression
Requirements: Students should be interested in the investigation of mitochondrial-dependent pathways involved in the progression of DMD and in the setting of preclinical studies with different DMD models to test the impact of their manipulation.
Heart and kidney inflammation in obesity: focus on the crosstalk between Dipeptidyl Peptidase-4 (DPP4), Glucagon-Like Peptide-1 Receptor (GLP-1R) and Angiotensin-converting enzyme 2 (ACE2) as risk factors and potential therapeutic targets in COVID-19
Requirements: Knowledge of the main techniques of cellular and molecular biology [cell cultures, gene silencing, gene and protein expression analysis (RT- Real Time PCR, Western blot)], and biochemical assays for the evaluation of biomarkers in fluids [eg. enzyme immunoassays (ELISA)].
The human-restricted duplicated isoform of the α7 nAChR, CHRFAM7A: its role in the modulation of the inflammatory response in neurodegenerative diseases
Requirements: Strong background in tissue culture, and in molecular biology, with emphasis on nucleic acid manipulation (plasmid costruction), mRNA quantitation (RT-PCR), Western blotting, and immunofluorescence. Experience with blood-derived cells culture.
Evaluation of the post Covid 19 effects on bone remodeling and bone fragility
Requirements: Molecular and cellular biology, enzyme immunoassays, real time PCR, evaluation of laboratory medicine parameters
Searching novel therapeutic approaches for the treatment of Rett syndrome: a focus on glial cells
Requirements: Previous experience in cellular and/or molecular biology and/or animal handling might be preferable
From exosomes to mini-guts: unveiling the pathogenesis of Crohn’s disease to personalize therapy
Requirements: Expertise in cell and molecular biology
Identification of genetic risk factors and interaction between genetic and non-genetic risk factors in pediatric multiple sclerosis
Targeting the tumor-escape mechanisms from immune-surveillance of metastatic colon rectal cancer
Requirements: Basic experience/knowledge of molecular and cellular biology techniques, flow cytometry. Advanced knowledge/experience on analytical approaches with computational methods. Availability of spending periods abroad for training and collaborations. Thesis/experience in computational medicine biology will be preferred to develop the analytic part of the project.
Role of endothelial dysfunction in the vulnerability for psychiatric diseases and in the mechanism of action of psychotropic drugs
Requirements: Expertize in gene and protein expression analyses, cell culture
Integrated epigenomic and transcriptomic analyses of tissue- and tumorinfiltrating lymphocytes
An innovative approach for the characterization of thyroid hormone action using iPSC from patients with resistance to thyroid hormones
Requirements: Previous experience with cell cultures and basic molecular analyses (eg, western blot or real time PCR) are preferred
Molecular mechanisms regulating insulin like growth factor (IGF) system in adrenocortical carcinomas
Requirements: Cell cultures; cell transfection and silencing; protein detection (western blot, co-/immunoprecipitation); cell imaging, cell proliferation, apoptosis, migration assays; nucleic acid extraction, PCR.
Role of pulmonary microbiota on lung cancer growth/progression and its targeting as a new strategy to improve immunotherapy efficacy
Requirements: Experience in a research lab in a field related to cell biology/molecular biology and immunology
New zebrafish models for preclinical research of pituitary tumors
Requirements: Attitude to teamwork, interest for preclinical research

Courses list

January 2022
Courses or activities Professor(s) ECTS Total hours Language
Optional
Improving your statistical and data handling skills in biomedical sciences 3 15 English
February 2022
Courses or activities Professor(s) ECTS Total hours Language
Optional
Quantification and characterization of extracellular vesicles in biomedical research 2 10 English
March 2022
Courses or activities Professor(s) ECTS Total hours Language
Optional
Basic and advanced techniques for optical microscopy in biological and preclinical research 2 12 English
April 2022
Courses or activities Professor(s) ECTS Total hours Language
Optional
Next Generation Sequencing: from the bench to the data analysis 3 15 English
Preclinical models in neuromuscular disorders 2 10 English
May 2022
Courses or activities Professor(s) ECTS Total hours Language
Optional
Flow cytometry in biomedical research 4 21 English
June 2022
Courses or activities Professor(s) ECTS Total hours Language
Optional
Modern approaches in studying gene expression 2 12 English
July 2022
Courses or activities Professor(s) ECTS Total hours Language
Optional
Translational potential of cell metabolism as new pharmacological target 3 15 English

Enrollment

Places available: 11

Call for applications

Please refer to the call for admission test dates and contents, and how to register.

Session: 1

Application for admission: from 28/06/2021 to 01/09/2021

Application for matriculation: from 27/09/2021 to 09/10/2021

Read the Call


Attachments and documents

Attachments to the call

Qualifications assessment

Evaluation Results

Session: 2

Application for admission: from 15/10/2021 to 08/11/2021

Application for matriculation: from 18/11/2021 to 22/11/2021

Read the Call


Attachments and documents

Attachments to the call

Extension

Attachment 1

Qualifications assessment

Evaluation Results

Research lines assigned