Flow cytometry in biomedical research

A.Y. 2020/2021
Course offered to students on the PhD programme in
Overall hours
Lesson period
May 2021
Flow cytometry is widely used in basic and translational research for investigating cellular phenotypes and functions at the level of single cells. The course is intended to provide students with notions in the terminology, concepts and approaches used in flow cytometry applications, needed for initiating flow cytometric studies, and allowing a critical interpretation of published flow cytometry results. The course will illustrate basic principles of flow cytometry, experimental design and quality control, critical issues in the pre-analytical and analytical phase, experimental design in polychromatic immunophenotype and functional cytometry. Examples of flow cytometric approaches applied to biomedical investigation will be presented through scientific seminars. Practical training on the analysis of flow data will be provided, in order to enable students to analyze flow data and present high quality data for publications. Wet lab practical training, illustrating instrumentation handling, cell preparation and staining, data acquisition and analysis, will be offered to students who are new to flow cytometry.
Maximum number of participants: 25
Assessment methods
Giudizio di approvazione
Assessment result
superato/non superato
How to enrol


The course enrolment deadline is usually the 25th day of the month prior to the start date. More specifically:

  • for courses starting in June 2021, until 25 May 2021
  • for courses starting in July 2021, until 25 June 2021
  • for courses starting in September 2021, until 30 August 2021.

How to enrol

  1. Access enrolment on PhD courses online service using your University login details
  2. Select the desired programme and click on Registration (Iscrizione) and then on Register (Iscriviti)

Ignore the option "Exam session date” that appears during the enrolment procedure.


For help please contact phd@unimi.it

Educational website(s)
On Appointment
Istituto Clinico Humanitas, Via A. ;anzoni 113, Rozzano, Milano