Biotechnology of reproduction

A.Y. 2015/2016
Lesson for
6
Max ECTS
48
Overall hours
SSD
VET/01 VET/10
Language
Italian
Learning objectives
Questo corso ha lo scopo di permettere agli studenti di acquisire le conoscenze tecniche indispensabili per affrontare sia lo studio molecolare dell'embrione, sia l'applicazione ai gameti e agli embrioni delle procedure di crioconservazione. Entrambi questi aspetti completeranno la preparazione di coloro che intendono approfondire i temi di biotecnologie della riproduzione trattati nei corsi fondamentali.

Course structure and Syllabus

Active edition
Yes
Responsible
Cryopreservation of gametes and embryos
VET/10 - VETERINARY CLINICAL OBSTETRICS AND GYNECOLOGY - University credits: 3
Lessons: 24 hours
Molecular embriology: principles and methods
VET/01 - VETERINARY ANATOMY - University credits: 3
Lessons: 24 hours
Professor: Pocar Paola
Molecular embriology: principles and methods
Syllabus
Lectures and laboratory practices:
1. model systems in embryology
2. cloning methods
3. transgenesis
4. semen and embryo sexing
5. stem cells
6. critical reading of field literature
7. methods for oocyte and preimplantation embryo manipulation
8. nucleic acid extraction from oocytes and preimplantation embryos
9. gene expression profiling in oocytes and preimplantation embryos
Cryopreservation of gametes and embryos
Syllabus
Lectures and practices:
- Fundamentals of cryobiology
- Cryopreservation of spermatozoa
- Cryopreservation of oocytes
- Cryopreservation of embryos
- Cryopreservation of gonadal tissue
- Vitrification
- BioBank organization
- Evaluation of cellular cold-induced damages
Lesson period
First semester
Lesson period
First semester