The course is aimed at giving the proper knowledge for the comprehension of biomolecular processes that are fundamental for cells and organisms function in both physiological and pathological conditions.
This course allows you to consolidate, deepen and broaden your knowledge of molecular biology with particular emphasis on RNA biology, genome organization and engineering. At the end of this course, you will be able to: -prove understanding and knowledge of the central aspects of molecular biology and their relevance and application to biomedical and biotechnology fields. -engage in critical weighing of recent biomedical applications of molecular biology and critically assess different methods of research.
NON-CODING REGULATORY RNAs - Small non coding RNAs (snRNA, snoRNA, siRNA, microRNA, piRNA): definition, classification, structure, mechanism of action and physio-pathological examples. - Long non coding RNAs: definition, classification, structure, mechanism of action and physio-pathological examples. DYNAMICS OF THE REPETITIVE ELEMENTS OF DNA IN CELL IDENTITY, DIFFERENTIATION, PATHOLOGIES AND THEIR EPIGENETIC ROLE. - Tandem repeats, satellite DNA, VNTR, Retrotrasposons, SINEs, LINEs: definition, classification, structure, mechanism of action and physio-pathological examples. 3D GENOME ARCHITECTURE - Genome hierarchy and organization. - Models for the genome tridimensional organization. - Functional role of nuclear domains and chromosome territories. - Techniques for the study of 3D genome structure. NEXT GENERATION SEQUENCING: APPLICATIONS AND DATA ANALYSIS - NGS: Principles and technologies. - NGS applications: whole genome sequencing; transcriptomics; capture sequencing (exome sequencing and custom target); epigenetics. - Single cell transcriptomics. - Elements of bioinformatic for NGS data analysis. GENOME ENGINEERING - Gene trapping, gene targeting, conditional knockout. - Homologous recombination, artificial restriction enzymes: Zinc Fingers Nucleases, TALEN, CRISPR/Cas9.
LABORATORY PRACTICE: Methodologies for RNA analysis.