Microbiological techniques

A.Y. 2017/2018
Lesson for
4
Max ECTS
40
Overall hours
Language
Italian
Learning objectives
Main purpose: to supply the cultural and operative instruments necessary for relating the acquired knowledge in the microbiological field to the microbiological methods and their application in the field of biotechnology.
Planning, organization and carriyng out of experimental analyses for the phenotypic and genotypic chracterization of microorganisms involved in the food industry.

Course structure and Syllabus

Active edition
Yes
Responsible
Laboratories: 16 hours
Lessons: 24 hours
Syllabus
¿ Lectures Isolation, selection and survey of microorganisms from heterogeneous matrices. Taxonomic identification of bacteria: phenotypic and genotypic characteristics. Planning and choise of experimental analyses for phenotypic and genotypic identification. Genetic organization in bacteria. The measurable characteristics of plasmidic and chromosomal DNA. The concepts of molecular genetics and their application in both basic and applied biotechnology (with particular regard to techniques that allow precise detection and amplification of DNA through the polymerase chain reaction or PCR) ¿ Laboratory exercises Culture media preparation. Use of autoclaves and filters for the sterilization of media and materials. Isolation techniques. Measurement of bacterial growth. Obtainment of pure cultures. Planning and carrying out of phenotypic analyses for bacterial identification and characterization. Extraction of chromosomal and plasmidic DNA and their study: separation and visualization through gel electrophoresis techniques; % GC evaluation, determination of % of genetic homology
Teaching methods
Appunti delle lezioni e delle esercitazioni Materiale didattico fornito dal docente
Lesson period
Second semester
Lesson period
Second semester
Assessment methods
Esame
Assessment result
voto verbalizzato in trentesimi