From 3d-culture and 3d-printing to organoids

A.Y. 2017/2018
Lesson for
8
Max ECTS
48
Overall hours
SSD
AGR/18 VET/01 VET/09
Language
English
Learning objectives
Cells in vivo are in a three-dimensional environment. Cells cultured in 3D models have biophysical characteristic and biomechanical signals, which more accurately simulate normal cell morphology, proliferation, differentiation and migrations. The objective of the course is to give a comprehensive review of the main aspects and methods for 3D cell models from 3D-culture and 3d-printing to organoids.

Course structure and Syllabus

Active edition
Yes
Responsible
Module: 3 D approach to cell culture
AGR/18 - ANIMAL NUTRITION AND FEEDING - University credits: 3
Lessons: 18 hours
Professor: Cheli Federica
Module: 3 D bioprinting and practicals
VET/09 - VETERINARY CLINICAL SURGERY - University credits: 2
Lessons: 12 hours
Professor: Acocella Fabio
Module: Cell interaction and organoids
VET/01 - VETERINARY ANATOMY - University credits: 3
Lessons: 18 hours
Professor: Gandolfi Fulvio
ATTENDING STUDENTS
Module: Cell interaction and organoids
Syllabus
Mechano-sensing
Mechanotransduction
Shearing
Stiffness materials and cell responses
How to select the adequate surface
Citoskeleton and ECM
Macro-crowding
Module: 3 D approach to cell culture
Syllabus
Cells cultured in 3D-model systems often acquire relatively large in vivo-like structures compared to the thickness of a 2D-monolayer of cells grown on standard plastic plates. Moreover, cells grow in a physiologically relevant environment, yet providing a challenge for assay chemistries originally designed for measuring events from monolayers of cells. 3D-culture methods depends on a number of factors, including the tissue you need to model and the particular research question you wish to explore. There is an unmet need for guidelines for design and validation of fit to purpose and effective assays useful for 3D-model systems. The course will provide the student with the understanding of the main aspects related to 3D-culture. Recommendations for factors to consider when verifying performance of cell health assays on 3D-culture models will also be presented.

Main topics:
3D-culture types
Methods and protocols for 3D-cultures
Critical factors to consider for each 3D-model system and cell type
Examples of applications of 3D-culture models
Practicals will be dedicated to 3D-culture
Module: 3 D bioprinting and practicals
Syllabus
3D-bioprinters: philosophy and application in medicine
Artificial and biological matrix
Fabrication process
Decellularization & Recellularization process
"Print your heart out 3D". How to design, create and implant and ad hoc organoid
NON-ATTENDING STUDENTS
Module: 3 D approach to cell culture
Syllabus
Cells cultured in 3D-model systems often acquire relatively large in vivo-like structures compared to the thickness of a 2D-monolayer of cells grown on standard plastic plates. Moreover, cells grow in a physiologically relevant environment, yet providing a challenge for assay chemistries originally designed for measuring events from monolayers of cells. 3D-culture methods depends on a number of factors, including the tissue you need to model and the particular research question you wish to explore. There is an unmet need for guidelines for design and validation of fit to purpose and effective assays useful for 3D-model systems. The course will provide the student with the understanding of the main aspects related to 3D-culture. Recommendations for factors to consider when verifying performance of cell health assays on 3D-culture models will also be presented.

Main topics:
3D-culture types
Methods and protocols for 3D-cultures
Critical factors to consider for each 3D-model system and cell type
Examples of applications of 3D-culture models
Practicals will be dedicated to 3D-culture
Lesson period
Second semester
Lesson period
Second semester
Assessment methods
Esame
Assessment result
voto verbalizzato in trentesimi
Professor(s)
Reception:
From Monday to Friday upon appointment
Office