Applications of biotech on medicine

A.Y. 2019/2020
Lesson for
Overall hours
MED/08 MED/40 MED/42 MED/43 MED/44 MED/46
Learning objectives
The Course of Biotechnological Applications in Medicine aims to illustrate the most recent applications of biotechnology in the fields of forensic medicine (to provide students with the basic elements of the national legal system and the legal and deontological rules related to responsibility in the exercise of the profession), reproductive medicine, environmental epigenetics, regenerative medicine and public health.
Understanding and analysis of basic biological phenomena and methodologic approaches to their study in relation to current or possible biomedical applications

Course structure and Syllabus

Active edition
MED/08 - PATHOLOGY - University credits: 0
MED/40 - OBSTETRICS AND GYNAECOLOGY - University credits: 0
MED/42 - HYGIENE AND PUBLIC HEALTH - University credits: 0
MED/43 - FORENSIC MEDICINE - University credits: 0
MED/44 - OCCUPATIONAL MEDICINE - University credits: 0
Lessons: 56 hours
Biotechnology of reproduction
Ovarian and endometrial cycle- spermatogenesis- conception
Implantation, develpment and function of the placenta - Fetal growth - Main embryo and trophoblast pathologies-pregnancy related diseases
Causes of infertility and clinical indications to assisted reproduction - in vitro assisted reproduction techniques
Crioconservation of gametes and reproductive tissues - stem cells for reproductive purposes - preimplantation diagnosis - Genomic imprinting
Invasive and non-invasive prenatal diagnosis techniques - reproductive techniques in couples with blood viral infections

Biotechnologies and public health.
- General principles. Hygiene and public health. Evolution of the "health" concept. Health and disease.
- Epidemiology methodology. Definition and objectives. Epidemiological studies. The molecular epidemiology approach..
- Prevention methodology. Definition and objectives. Molecular biology in the prevention field.
Vaccines and vaccination strategies.
- Biotechnologies and development of new generation vaccines.
Public health topicality.
- Emerging and re-emerging infectious diseases.
- New technologies as tools to face up to new health emergences.

Forensic medicine, definition; legal principles: outline (the Constitution of the Republic, outline of criminal and civil law with the different characteristics of the criminal and civil trial); definition of a crime; office and lawsuit prosecution; the juridical figure of the biotechnologist in the exercise of the profession; the legal notion of a biological sample; the complaint, the lawsuit; report and crime report; private violence; Professional secret; consent to the health act; professional responsibility in the exercise of the profession; the causal link; the damage; the Legislative Decree n. 196 of 30/6/2003 (Privacy Law) and subsequent amendments; the code of ethics

Introduction and General Concepts.
Technologies for DNA Methylation and Histone Modification Analyses.
Epigenetics: Environmental Instructions for the Genome.
Design of Epidemiology Studies and Epigenetic Aging
Health Correlates of Epigenetic Alterations. Genomic Imprinting and Epigenetic Inheritance.
Diet and Epigenetic States. Epigenetics and Cancer.
Epigenetics Alterations of Cardiovascular, Respiratory and Neurological Diseases.
Non-coding RNAs.

Cell and tissue culture methods, laboratory and requirements.
Tissue and cells bank, cryo- preservation
Cell culture in biomedical diagnosis ( amniocytes culture, fibroblasts culture)
Cell culture in biomedical research
tumor invasion and metastasis
signal transduction
Hematopoietic cells
Tumor stem cell
Cell migration: molecular mechanisms and in vitro models
Cell death: methods to study apoptosis and necrosis
Integrins extracellular matrix, growth factors: characteristic and dynamic changes
during tumour progression; and targets for new cancer therapeutics.
Lesson period
Second semester
Lesson period
Second semester
Assessment methods
Assessment result
voto verbalizzato in trentesimi
On Appointment
Istituto Clinico Humanitas, Via A. ;anzoni 113, Rozzano, Milano
Monday 12.00-13.00
Interhospital Pathology Division, Science and Technology Park, Via Fantoli 16/15, 20138 Milano