Applied Microbiology

A.Y. 2019/2020
Lesson for
6
Max ECTS
48
Overall hours
SSD
BIO/19
Language
Italian
Learning objectives
The main objective of the course is to provide students with the basis to face the study of the major fundamentals of microbiology including its applications in the diagnostic and pharmaceutical fields.

Course structure and Syllabus

Linea AK
Active edition
Yes
Responsible
BIO/19 - MICROBIOLOGY - University credits: 6
Lessons: 48 hours
Professor: Sperandeo Paola
Lesson period
First semester
Linea LZ
Active edition
Yes
Responsible
BIO/19 - MICROBIOLOGY - University credits: 6
Lessons: 48 hours
Professor: Polissi Alessandra
ATTENDING STUDENTS
Syllabus
1) Overview of microbiology and its applications.
2) Overview of cell structure: prokaryotes and eukaryotes. Bacteria and Archaea. Cytoplasmic membrane, structure and function. Bacterial transport systems.Cell wall of prokaryotes. Outer membrane of Gram-negative bacteria. Capsules. Flagella and bacterial motility. Surface structure for adhesion: pili. The nucleoid. Structure and organization of bacterial chromosome. Cytoplasm. Ribosomes. Cell inclusions. Endospores.
3) Microbial growth and microbial growth control. Principles of microbial nutrition. Liquid and solid media, growth conditions. Methods for sterilization. Disinfectants and antiseptics.
4) Nutrition and metabolism. Overview of metabolism. Fermentation and substrate level phosphorylation. Aerobic and anaerobic respiration. Chemiolithotrophy. Photosynthesis: anoxygenic photosynthesis. Autotrophic CO2 fixation. Nitrogen metabolism. Nitrogen fixation.
5) Microbial genetics. Mutations and mutants. Horizontal gene transfer mechanisms. Overview of regulation of gene expression.
6) Viruses. Overview of bacterial viruses. Lytic cycle and lysogeny.
7) Selected examples of microorganisms relevant in clinic and industry
8) Antibiotics. Structure and mechanisms of action. Minimal inhibitory concentration (MIC). Overview on resistance mechanisms.
NON-ATTENDING STUDENTS
Syllabus
1) Overview of microbiology and its applications.
2) Overview of cell structure: prokaryotes and eukaryotes. Bacteria and Archaea. Cytoplasmic membrane, structure and function. Bacterial transport systems.Cell wall of prokaryotes. Outer membrane of Gram-negative bacteria. Capsules. Flagella and bacterial motility. Surface structure for adhesion: pili. The nucleoid. Structure and organization of bacterial chromosome. Cytoplasm. Ribosomes. Cell inclusions. Endospores.
3) Microbial growth and microbial growth control. Principles of microbial nutrition. Liquid and solid media, growth conditions. Methods for sterilization. Disinfectants and antiseptics.
4) Nutrition and metabolism. Overview of metabolism. Fermentation and substrate level phosphorylation. Aerobic and anaerobic respiration. Chemiolithotrophy. Photosynthesis: anoxygenic photosynthesis. Autotrophic CO2 fixation. Nitrogen metabolism. Nitrogen fixation.
5) Microbial genetics. Mutations and mutants. Horizontal gene transfer mechanisms. Overview of regulation of gene expression.
6) Viruses. Overview of bacterial viruses. Lytic cycle and lysogeny.
7) Selected examples of microorganisms relevant in clinic and industry
8) Antibiotics. Structure and mechanisms of action. Minimal inhibitory concentration (MIC). Overview on resistance mechanisms.
Lesson period
First semester
Lesson period
First semester
Assessment methods
Esame
Assessment result
voto verbalizzato in trentesimi