Instrumentations and fundamentals of main analytical and separation techniques. Qualitative and quantitative problem solving.
At the end of the course the student will be able to solve qualitative and quantitative analytical problems with the most suitable technique. He will develop the expertise to characterize simple organic structure from the corresponding IR, NMR and MS spectra.
Qualitative and quantitative experiences on the main spectroscopic (AAS, AES, UV-Vis, IR, fluorescence) and chromatographic (GC, HPLC) techniques presented during the lectures.
Modulo: Chimica Analitica II
Course content Principles of spectrophotometric and photoelectronic methods. Atomic adsorption and emission. Molecular spectroscopy: UV-vis, IR and Raman, fluorescence and phosphorescence. Mass spectrometry. Nuclear Magnetic Resonance: monodimensional 1H- and 13C-NMR techniques. Spectrum parameter analyses: chemical shift and coupling constant. Overhauser effect. Principles and methods of chromatographic techniques (GC and HPLC). Set-up of qualitative and quantitative analyses. Exercises on structure disclosing based on NMR, IR, UV and MS spectra. Practices of theoretical concepts. Numerical exercises on quantitative analyses involving spectroscopic and chromatographic techniques.
Suggested prerequisites Positive assessment of analytical chemistry I
Assessment method [required] (use at least 2 lines): The exam will be constitute by: - Written test of 5 questions/exercises based on exercises and laboratory experiments - Written test divided into two parts: o 6 questions of short solution which, only if correctly solved, allow to pass to the second part o 5 questions on theory and spectra interpretation The two tests will be held in different day but they will be assessed with a single mark.
Language of instruction Italian
Attendance Policy Strongly suggested, compulsory frequency at the laboratory