Pharmacological biomolecular sciences, experimental and clinical

Dottorati
Doctoral programme (PhD)
A.Y. 2019/2020
Course class
Classe per Dottorati di Ricerca
Study area
Science and Technology
Doctoral programme (PhD)
3
Years
Dipartimento di Scienze farmacologiche e biomolecolari - Via G. Balzaretti, 9 - 20133 Milano
English
The doctoral programme, a third cycle programme, makes the most of the multidisciplinary knowledge of the teaching staff to train biomedical experts in the most modern aspects of pharmacological research. In particular, the areas of interest are:
Basic research: the study of key mechanisms and molecules in physiopathological processes to identify new pharmacological targets and/or new markers predictive of disease and therapeutic effectiveness. Research involves: 1) the analysis of molecular and cellular aspects of complex physiological processes, 2) the study of pathogenic mechanisms in diseases, 3) the study of biological markers of use in translation of preclinical research to humans.
Research applied to the pharmacological-toxicological study of active ingredients: 1) analisi analysis of the activity of active principles, including biological drugs under development or in clinical use, 2) analysis of foods, food supplements and therapeutic plant derivatives, 3) analysis of xenobiotic activity.
Research applied to the creation of innovative systems for studying pathologies and developing drugs and evaluation of their effects on the population. Basic research on: 1) the development of cellular and animal models also through genetic engineering methodologies, 2) optimising preclinical protocols for simple and complex diseases, including co-morbidities 3) in silico approaches to the study of new drug-toxicology targets, 4) the use of databases and population studies also to assess drug use and outcomes in terms of clinical events.
Technical-scientific collaboration for revising documentation on active compounds and plant protection products and for drug safety and with other clinical centres.
In conclusion, students will develop competencies applicable to various areas of academic and industrial research, allowing effective "bench to bed" translation and a multidisciplinary approach essential for public health, with significant socio-economic impact on health.
Dipartimento di Scienze farmacologiche e biomolecolari - Via G. Balzaretti, 9 - 20133 Milano
Title Professor(s)
Drug repurposing: a novel strategy to unveil pro-regenerative agents for demyelinating neurological disorders
Requirements: The ideal candidate must demonstrate laboratory experience in pharmacology, biochemistry and molecular biology, cell culture, and, possibly in vivo preclinical models. Expertize in the field of neurodegenerative diseases
Design and characterization of the “quadripartite synapse” in vitro
Requirements: Good knowledge of basic molecular and cellular biology techniques and abilities in routine chemical and biochemical methods in the lab (pcr, western blot, titrations, solutions)
The c-Jun N-terminal Kinase 3 is a key player in AD’s synaptopathy
Requirements: Good knowledge in basic molecular and cellular biology techniques and in routine chemical and biochemical methods in the lab (pcr, western blot, titrations, solutions)
Investigation of the cognitive dysfunctions in an experimental model of anorexia nervosa
Requirements: Expertise in gene and protein expression studies and in animal manipulation. Expertise in basic morphological dendritic spine analyses using confocal microscopy
Unveiling the role of serotoninergic system in the development of CNS pathologies through genetic and pharmacological approaches
Requirements: mRNA extraction and analysis (RT- real time PCR), protein extraction and analysis (western blot, ELISA)
Impact of stress exposure during adulthood on cognitive performance: influence of the pharmacological treatment
Requirements: mRNA extraction and analysis (RT- real time PCR), protein extraction and analysis (western blot, ELISA)
Targeting brain cholesterol transport in Alzheimer’s Disease
Requirements: Laboratory experience (biochemical analyses, in vitro and animal studies).
Genetic lecithin:cholesterol acyltransferase (LCAT) deficiency: towards precision medicine for a rare heterogeneous disorder
Requirements: Laboratory experience (in vitro and animal studies).
Dissecting the mechanisms responsible for platelet heterogeneity
Requirements: Expertise in cell and molecular biology, flow cytometry and (confocal) microscopy is an advantage for the position. Attitude to work both independently and as part of a team. Specific expertise in the field of cardiovascular diseases will represent a significant add-on to the candidate’s CV
Protein S-glutathionylation in calcific aortic valve disease
Requirements: It is required: experience in human primary cell cultures; good knowledge of molecular biology techniques; attitude to work both independently and as part of a team. Specific expertise in the field of cardiovascular diseases will represent a significant add-on to the candidate’s CV
Identification and study of new candidate genes involved in the development of GnRH neurons and in the pathogenesis of associated reproductive disorders.
Requirements: The candidate should have good knowledge of developmental neurobiology. He/she should also have laboratory experience in cell and molecular biology and preferably of embryology/histology
Metabolomic and lipidomic approaches to study pathologies characterized by metabolic dysfunction.
Requirements: Knowledge of lipid, glucose and amino acid metabolism. Quantitative analyses by liquid chromatography tandem mass spectrometry of metabolites involved in different metabolic pathways. Metabolic flux analysis.
Anti-PCSK9 monoclonal antibodies to regulate the binding of PCSK9 to LDL.
Requirements: Competence in the use of cell cultures, of basic molecular biology and electrophoretic techniques; in working with animal models (genetically modified mice), attitude to work independently.
Familial dyslipidaemias in Italy: spectrum of mutations, clinical manifestations and influence of environmental factors
Requirements: Knowledge of the cellular, molecular, and clinical aspects of genetic-based lipid disorders. Skills in building and running a database. Knowledge of SPSS and SAS software.
Use of mendelian randomization studies to identify possible pharmacological targets for the treatment of the cardiovascular disease
Requirements: Knowledge of the molecular, biological, and clinical aspects of cardiovascular diseases, knowledge of pharmacology. Skills in building and running a database. Knowledge of SPSS and SAS software.
Evaluation of polypharmacy in elderly patients and potential for a deprescribing approach
Requirements: Knowledge of pharmacology, and of how to interrogate databases, skills in building and running a database. Knowledge of SPSS and SAS software.
Insights into the role of glial cells and of metabolic alterations in multiple sclerosis-related increased susceptibility to trigeminal pain
Requirements: The ideal candidate must demonstrate laboratory experience in pharmacology, biochemistry and molecular biology and experience on in vivo preclinical and behavioral studies. Expertise in the field of basic mechanisms of pain.
Interplay between lipoprotein metabolism and microbiota in atherosclerosis development
Requirements: DNA and RNA extraction, PCR and qPCR analysis, protein extraction and analysis (Western blot, ELISA)
Preclinical development of near-infrared fluorescent (NIR) probes labelling the tumor-associated inflammation and their use in augmented reality applied to surgical oncology
Requirements: The Ph.D. student should be acquainted with the use of basic molecular and cellular biology tools, he/she should have experience in the use of fluorescent and bioluminescent reporters, and should have acquired the basic technical skills for laboratory animal manipulation. In addition, it should be familiar with the common Office Package and commonly used statistical programs
Development of optical imaging systems for the measurements of molecular events during neurodegeneration
Requirements: The Ph.D. student should be acquainted with the use of basic molecular and cellular biology tools, he/she should have experience in the use of fluorescent and bioluminescent reporters, and should have acquired the basic technical skills for laboratory animal manipulation. In addition, he/she should be familiar with the common Office Package and commonly used statistical programs.
Neuroinflammation as a target for neuroprotective drugs
Requirements: The PhD student should be acquainted with the use of basic molecular and cellular biology and biochemistry techniques, he/she should have the basic technical skills for laboratory animal manipulation. In addition, it should be familiar with the common Office Package and commonly used statistical programs
Precision medicine to study and prevent metabolic adverse effects induced by second generation antipsychotic drugs (SGAs): pharmacogenetic profiling in patients and mechanistic analysis of the effect of genetic variants in vitro
Requirements: Molecular, cellular and biochemical expertise; Bibliographic searching basic skills (Ex. Pubmed)
The multidisciplinary approach to management of therapy in the clinical practice: from the post-marketing surveillance to basic pharmacology
Requirements: Pharmacology and Pharmacovigilance basic skills. Knowledge of Office Package (Ex. Word, Excel, Access, Power Point). Bibliographic searching basic skills (Ex. Pubmed). Knowledge of statistical analysis software (Ex. SPSS, SAS)
Lipid uptake and metabolism as innovative target in breast cancer
Requirements: Biochemical and cell biology expertises.
Pharmacological modulation by a SGLT2 inhibitor in a mouse model of nafld/nash and possible metabolic implications
Requirements: Biochemical, cell biology, pharmacology and lipidomics expertises.
Epigenetics of energy metabolism in obesity and type 2 diabetes
Requirements: Knowledge of energy metabolism, chromatin accessibility studies and transcriptional regulation. Quantitative analyses by liquid chromatography tandem mass spectrometry of metabolites involved in different metabolic pathways. Metabolic flux analysis.
Extracellular Vesicles in the pathogenesis of Frontotemporal Dementia
Requirements: Good knowledge in cellular and molecular biology techniques. Good preparation in the neuroscience field. Interest for working with cellular models
Identification of novel pharmacological approaches to the cure of chronic viral infections
Requirements: Strong interest in the areas of molecular virology and antiviral drugs. Solid experience in standard laboratory techniques of cell culture and molecular biology (purification and quantification of viral antigens and nucleic acids, PCR. Real-time PCR, Elisa, Western Blot) and recombinant DNA technology. Familiarity with optical microscopy (immunofluorescence, confocal, etc)
Botanicals with anti-inflammatory activity in skin diseases
Requirements: Expertise related to extracts preparation from natural source, bio-guided fractionation, cell culture, transient transfections, ELISA, real time RT-PCR, use of LC-MS/MS instruments.
Botanicals as anti-inflammatory agents in human gastric epithelial cells
Requirements: Competences in the preparation of extracts from plant material, bio-guided fractionation, cultivation and manipulation of eukaryotic cell cultures, transient transfections, ELISA, extraction of RNA and real time PCR, western blotting, routine biochemical assays
Study of the synapse-to-nucleus signalling in Alzheimer's disease
Requirements: We look for a highly motivated person who is really interested in neurobiology and generally in neuroscience. We require that the candidate is able to develop independently on his/her research project and to work in collaboration with the lab members. Expertise in cellular and molecular biology and availability to work with in vivo animal models are required.
Single and repeated exposure to the psychostimulant cocaine during adolescence: behavioral and molecular correlates
Requirements: Expertise in gene and protein expression studies. Expertise in basic morphological dendritic spine analyses using confocal microscopy
Role of alpha-synuclein and LRRK2 in Levodopa-induced dyskinesia
Requirements: Knowledge and a genuine interest in neuroscience; experience in performing laboratory work independently; experience in performing a long laboratory project, e.g. a bachelor or master thesis project; good collaborative and social skills and an open-minded mind-set.
Role of lysosomal acid lipase in immunity
Requirements: Basic knowledge of biochemical, cellular and molecular biology techniques
Role of the G protein-coupled receptor GPR17 in rewiring oligodendrocyte lipid metabolism in models of multiple sclerosis
Requirements: Experience in the neuroscience field; experience in vitro on primary cultures from rat or mouse brain; skills in molecular biology (extraction of nucleic acids, qRT-PCR), immunocytochemistry, immunofluorescence and microscopy; experience in manipulation of rats or mice
Identification of new pharmacological targets to promote remyelination in models of multiple sclerosis
Requirements: Experience in the neuroscience field; experience in vitro on primary cultures from rat or mouse brain; skills in molecular biology (extraction of nucleic acids, qRT-PCR), immunocytochemistry, immunofluorescence and microscopy; experience in manipulation of rats or mice.
Unrevealing physiopathological mechanisms of schwannoma development
Requirements: Expertise in basic cell biology, focused to the primary culture of nervous of neuronal and glial cells,(Schwann cells and DRG neurons) stem cells. Skills and experiencein co-culturing, and in vitro myelination. Skills in cell biology (tumorigenesis assay, flow cytometry), molecular biology (transfections), immunocytochemistry, imaging techniques, patch clamp electrophysiology.
Cellular and molecular mechanisms of cardiovascular and metabolic diseases: elucidating the role of individual predisposition to aging-associated low-grade inflammation
Requirements: Experience in cellular and molecular biology in the life science field. Basic knowledge of molecular mechanisms of chronic diseases.
Statins and statin-associated muscle symptoms/de novo diabetes mellitus: exploring new targets of statins by an in silico plus in vitro combined approach
Requirements: Experience in cellular and molecular biology in the life science field. Basic knowledge of pharmacology.
Development of peptidomimetics for the treatment of Alzheimer Disease.
Requirements: We look for a highly motivated person who is really interested in neurobiology and generally in neuroscience. We require that the candidate is able to develop independently on his/her research project and to work in collaboration with the lab members. Expertise in cellular and molecular biology and availability to work with in vivo animal models are required.
Analysis of the crosstalk between ADAM10 and the NMDA receptor subunit GluN2A in synaptic plasticity phenomena
Requirements: We look for a highly motivated person who is really interested in neurobiology and generally in neuroscience. We require that the candidate is able to develop independently on his/her research project and to work in collaboration with the lab members. Expertise in cellular and molecular biology.
Deciphering the role of ADAM10 and CAP2 in Age-related Accumulation of deficits
Requirements: We look for a highly motivated person who is really interested in neurobiology and in the study of the correlation between actin cytoskeleton and mitochondrial function during ageing. We require that the candidate is able to develop independently on his/her research project and to work in collaboration with the lab members. Expertise in cellular and molecular biology
In vitro genotoxicological evaluation of environmental pollutants: a new integrate testing strategy
Requirements: Laboratory techniques acquired: cell cultures, genotoxicity test, molecular biology techniques
Persistent side-effects of drugs: Inhibitors of the enzyme 5alpha-reductase and antidepressant drugs (SSRI)
Requirements: Experience with animal models, molecular biology techniques
Diabetic encephalopathy and peripheral neuropathy: sex dimorphic role of neuroactive steroids
Requirements: Experience with animal models, molecular biology techniques
Unravelling the role of the novel mitochondrial regulator/RNA binding protein Zc3h10 in diabetes.
Requirements: RNA metabolism and regulation. Energy metabolism. Quantitative analyses by liquid chromatography tandem mass spectrometry of metabolites involved in different metabolic pathways. Metabolic flux analysis.
Role of lipid metabolism in immunometabolic reprogramming of innate and adaptive immune cells.
Requirements: Knowledge of the cellular, molecular, and clinical aspects of metabolic disorders and of immunology. Knowledge of electrophoretic techniques. Experience in working with experimental models, attitude to work independently.
Unveiling the role of the asialoglycoprotein receptor type 1 in atherosclerosis
Requirements: Knowledge of the cellular, molecular, and metabolic disorders and of immunology. Knowledge of electrophoretic techniques. Experience in working with experimental models, attitude to work independently.
Dissecting the role of pancreatic PCSK9 on insulin secretion and glucose metabolism.
Requirements: Knowledge in cellular and molecular biology, fluorescence microscopy techniques and/or animal models of diseases
The role of TDP43 in the pathogenesis of amyotropic lateral sclerosis
Requirements: Good knowledge of basic neuroscience and motor neuron diseases. Interest and aptitude for molecular and cellular studies on motor neuron diseases in cells and animal models. Basic knowledge on the mechanisms of toxicity of the misfolded proteins
Protective mechanisms against neurotoxic proteins in motoneuron diseases
Requirements: Good knowledge of basic neuroscience and motor neuron diseases. Interest and aptitude for molecular and cellular studies on motor neuron diseases in cells and animal models
Role of microRNAs in animal models of aging/frailty associated to cognitive impairment
Requirements: Basic knowledge of qPCR and Western blot tecniques
Investigating the functional and molecular consequences of stress exposure in utero toward the identification of novel therapeutic strategies for the treatment of psychiatric disorders.
Requirements: Knowledge of behavioral analyses for studying animal models to psychiatric disorders; basic knowledge of molecular biology methodologies, such as real time PCR, and protein analysis.
Role of inflammation, gut microbiome and bacteria metabolites in the long-term effect of early life stress exposure: toward the identification of novel biomarkers.
Requirements: Knowledge of basic molecular biology methodologies, such as real time PCR and transcriptomics, miRNOMics and metagenomics. Good expertise in bioinformatic analyses
Analysis of the early events driving metabolic adaptation of normal epidermal cells into squamous cell carcinoma (SCC)
Requirements: Biochemical and cell biology expertises.
COXTRAN: a new class of non steroidal anti-inflammatory drugs
Requirements: Experience in molecular pharmacology, cellular culture and transfection
Extracellular Vesicles miRNA at the crossroad between atherosclerosis and PCSK9
Requirements: Highly motivated person with a clear interest in the field of pathophysiology of atherosclerosis. Be able to work both independently and within a multinational group and be available to spend few months abroad. The knowledge of few techniques of molecular biology is required
The role of lysophagy in the pathogenesis of Spinal and Bulbar muscular atrophy and other polyQ diseases
Requirements: Good knowledge in cellular and molecular biology techniques. Good preparation in the neuroscience field. Interest for working with cellular and animal models.
New pharmacological targets for the treatment of pulmonary disease
Requirements: Basic knowledge of cell culture maintenance and of models of respiratory system pathologies; use of immunoassays and mass spectrometry for the determination of molecular biomarkers
Characterization, mechanism of action and activity optimization of antimicrobial peptide derivatives of Temporin B against Gram-negative pathogens for the development of new antibacterial drug
Requirements: Knowledge of basic microbiology laboratory techniques, bacterial genetics and molecular biology. Willingness to carry out part of the activity in the laboratory of Inorganic Chemistry of the Department of Pharmacological Sciences (Unimi).
Evaluation of risk factors distribution in Italian populations, nutritional, enviromental and genetic factors with Artificial Intelligence methods
Requirements: Knowledge of pharmacology, and of how to interrogate databases, skills in building and running a database. Knowledge of SPSS and SAS software.
Study of the role of CD4 T memory stem cells in HIV latency and strategies for reactivation
Requirements: Strong interest in the areas of molecular virology and antiviral drugs. Solid experience in standard laboratory techniques of cell culture and molecular biology (purification and quantification of viral antigens and nucleic acids, PCR. Real-time PCR, Elisa, Western Blot) and recombinant DNA technology. Familiarity with optical microscopy (immunofluorescence, confocal, etc)

Enrollment

Places available: 16


Call for applications: how to do, attachment 1, Qualifications assessment and exam schedule


Application period: from 05-24-2019 to 06-24-2019
Enrolment period: from 09-23-2019 to 09-27-2019
Please note:
Allegato 1: Decreto di ampliamento dei posti e delle borse messi originariamente a concorso.