Il corso si propone di presentare e discutere i criteri e metodi per la formulazione di un progetto biotecnologico, basato sull'utilizzazione di cellule vegetali o piante, per produzioni di interesse industriale o per la tutela ambientale. E' prevista almeno una visita ad una azienda/centro di ricerca del settore.
Section 1 This section focuses on the technical tools and theoretical basis of plant/ algae biotechnological improvements for biofuel or food production. The different constructs for plant and algae genetic engineering together with the molecular basis of Agrobacterium-mediated transformation are discussed first. The different elements of expression vectors, including constitutive and inducible promoters, enhancers, reporter genes, replication origin and marker genes, together with the strategies to create marker-free transgenes are described. Then the organisms and the strategies used to produce biofuels of first, second and third generation are described, with several examples of transgenic plants and algae with improved biofuel production capacity. The third part will examine the traits of plants modified by transgenesis or mutagenesis which are commercially available: herbicide tolerance, pathogen resistance, improved nutritional content... etc. Section 2 This section approaches two fundamental questions of metabolic engineering: 1) which factors regulate metabolic fluxes and 2) what are the best strategies to modify the yields in a certain product (altering metabolite concentrations or fluxes). In order to tackle the questions we shall make use of the tools provided by the Metabolic Control Analysis theory. We shall discuss the limits due to supply/demand of metabolic intermediates as well as the evidence in favor of the so-called Universal method (parallel activation) to increase fluxes and the implications for manipulation strategies. Several case studies (and the relative effectiveness, merits and limits) of biotechnological interventions will be discussed, among which: inactivation of allergens and toxins and the production of material for industrial use (starches, sugars, lipids, vitamins, aminoacids, dyes, drugs, flavourings...etc.).
Informazioni sul programma
Full programs and lecture slides available at the ARIEL website
The examination is oral and consists of several questions on each section of the program
Materiale di riferimento
Literature and lecture notes concerning Section 1 are provided during the lectures. Concerning Section 2, some bibliographic material is listed below. Further material is presented and made available during the lectures  Fell, Understanding the control of Metabolism, Portland Press (1997)  Dennis/Turpin, Plant Metabolism (1998) Longman; nuova edizione.  Lea/Leegood, Plant Biochemistry and Molecular Biology (1993) Wiley & sons.