Bioimaging

A.A. 2018/2019
Insegnamento per
6
Crediti massimi
56
Ore totali
SSD
FIS/03 FIS/07
Lingua
Inglese

Struttura insegnamento e programma

Edizione attiva
Responsabile
FIS/03 - FISICA DELLA MATERIA - CFU: 0
FIS/07 - FISICA APPLICATA (A BENI CULTURALI, AMBIENTALI, BIOLOGIA E MEDICINA) - CFU: 0
Esercitazioni: 16 ore
Lectures: 40 ore
Programma
The topics covered by the lectures will be:
· Optical Imaging
o Introduction to optical microscopy: bright-field, dark-field, phase contrast,
autofluorescence /fluorescence
o Imaging techniques with fluorescent probes: confocal microscopy, FRAP, FRET, STED,
TIRF, two-photon excitation microscopy
· Electron microscopy & spectromicroscopy
o Scanning electron microscopy (SEM), Energy Dispersive X-Ray Spectroscopy (EDX),
Wavelength Dispersive Spectroscopy (WDS)
o Transmission Electron Microscopy (TEM), Electron Energy Loss Spectroscopy (EELS)
· Scanning probe microscopy
o Scanning Tunnelling Microscopy (STM)
o Atomic Force Microscopy (AFM)
· Application of bio-imaging
o Lab-on-chip and Cell-on-chip systems

The course includes 16 hours of laboratory in which the students will experience: confocal
microscopy, electron microscopy, atomic force microscopy, design of a lab on chip device.
Periodo
Primo semestre
Modalità di valutazione
Esame
Giudizio di valutazione
voto verbalizzato in trentesimi
Siti didattici
Docente/i
Ricevimento:
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