From 3d-culture and 3d-printing to organoids

A.A. 2018/2019
Insegnamento per
8
Crediti massimi
48
Ore totali
SSD
AGR/18 VET/01 VET/09
Lingua
Inglese
Obiettivi formativi
Cells in vivo are in a three-dimensional environment. Cells cultured in 3D models have biophysical characteristic and biomechanical signals, which more accurately simulate normal cell morphology, proliferation, differentiation and migrations. The objective of the course is to give a comprehensive review of the main aspects and methods for 3D cell models from 3D-culture and 3d-printing to organoids.

Struttura insegnamento e programma

Edizione attiva
Responsabile
Moduli o unità didattiche
Module: 3 D approach to cell culture
AGR/18 - NUTRIZIONE E ALIMENTAZIONE ANIMALE - CFU: 3
Lezioni: 18 ore
Docente: Cheli Federica

Module: 3 D bioprinting and practicals
VET/09 - CLINICA CHIRURGICA VETERINARIA - CFU: 2
Lezioni: 12 ore
Docente: Acocella Fabio

Module: Cell interaction and organoids
VET/01 - ANATOMIA DEGLI ANIMALI DOMESTICI - CFU: 3
Lezioni: 18 ore
Docente: Gandolfi Fulvio

STUDENTI FREQUENTANTI
Informazioni sul programma
Detailed information on the programme are reported in the specific sections of the modules.
Prerequisiti e modalità di esame
Oral test
Metodi didattici
Classical lectures, Laboratory direct interactions that will be integrated by webinars and on-line activities
Module: Cell interaction and organoids
Programma
Mechano-sensing
Mechanotransduction
Shearing
Stiffness materials and cell responses
How to select the adequate surface
Citoskeleton and ECM
Macro-crowding
Metodi didattici
Classical lectures,practical classes, webinars
Materiale didattico e bibliografia
Selected review articles, web-sites and webinars
Module: 3 D approach to cell culture
Programma
Cells cultured in 3D-model systems often acquire relatively large in vivo-like structures compared to the thickness of a 2D-monolayer of cells grown on standard plastic plates. Moreover, cells grow in a physiologically relevant environment, yet providing a challenge for assay chemistries originally designed for measuring events from monolayers of cells. 3D-culture methods depends on a number of factors, including the tissue you need to model and the particular research question you wish to explore. There is an unmet need for guidelines for design and validation of fit to purpose and effective assays useful for 3D-model systems. The course will provide the student with the understanding of the main aspects related to 3D-culture. Recommendations for factors to consider when verifying performance of cell health assays on 3D-culture models will also be presented.

Main topics:
3D-culture types
Methods and protocols for 3D-cultures
Critical factors to consider for each 3D-model system and cell type
Examples of applications of 3D-culture models
Practicals will be dedicated to 3D-culture
Metodi didattici
Classical lectures, practical classes, webinars
Materiale didattico e bibliografia
3D Cell Culture Methods and Protocols. Editors: Haycock, John (Ed.). Humana press. 2011.
Selected review articles, web-sites and webinars
Module: 3 D bioprinting and practicals
Programma
3D-bioprinters: philosophy and application in medicine
Artificial and biological matrix
Fabrication process
Decellularization & Recellularization process
"Print your heart out 3D". How to design, create and implant and ad hoc organoid
Metodi didattici
Classical lectures,practical classes, webinars
Materiale didattico e bibliografia
Selected review articles, web-sites and webinars
STUDENTI NON FREQUENTANTI
Prerequisiti e modalità di esame
Oral test
Periodo
Secondo semestre
Periodo
Secondo semestre
Modalità di valutazione
Esame
Giudizio di valutazione
voto verbalizzato in trentesimi
Docente/i
Ricevimento:
appuntamento via mail
Ricevimento:
previo appuntamento
Sede di Via Trentacoste, 2
Ricevimento:
Dal Lunedì al Venerdì su appuntamento
Studio