Applied biocatalysis

A.A. 2019/2020
Insegnamento per
6
Crediti massimi
52
Ore totali
SSD
CHIM/11
Lingua
Inglese
Obiettivi formativi
The course aims to provide students with theoretical knowledge and applicative skills about biocatalytic processes. Biocatalysis is the use of natural catalysts (enzymes) to perform reactions of interest in all fields of green chemistry. To understand and develop a biocatalytic process is thus necessary to gather tools across different biotechnological techniques.

The main objectives of this course are:

- Understanding the advantages of biocatalysis in the context of the bioeconomy;

- Comprehending why biocatalysis is strategic in a bio-based industry context;

- Learning how to apply biotechnological techniques (mostly learned in other courses) for the evolution of enzymes or microbial cells into efficient biocatalysts;

- Knowing the methodologies (protein engineering, metabolic engineering, immobilization, continuous processes) useful for making a biocatalytic process of industrial interest;

- Learning from consolidated and/or more innovative and recent successful examples (especially in the agri-food and energy fields) how a biocatalytic process is developed and optimized;

- Assessing the most suited technologies for intensifying a biocatalytic process
The course aims to provide students with theoretical knowledge and applicative skills about biocatalytic processes. Biocatalysis is the use of natural catalysts (enzymes) to perform reactions of interest in all fields of green chemistry. To understand and develop a biocatalytic process is thus necessary to gather tools across different biotechnological techniques.

Struttura insegnamento e programma

Edizione attiva
CHIM/11 - CHIMICA E BIOTECNOLOGIA DELLE FERMENTAZIONI - CFU: 6
Laboratori: 8 ore
Lezioni: 44 ore
STUDENTI FREQUENTANTI
Programma
- Introduction to biocatalysis: why? how? The myth and the reality (0.5 CFU);
- Biocatalysis: how enzymes work (0.5 CFU);
- Biocatalysis at work: biotransformation of feedstocks into added-value molecules (0.5 CFU);
- Enzyme discovery and high throughput fermentation (0.5 CFU);
- Biocatalyst preparation (0.5 CFU):
- whole cells or isolated enzymes?
- immobilization;
- Techniques in biocatalysis: how biocatalysts can be improved? (1.0 CFU);
- protein engineering, directed evolution
- metabolic engineering
- Examples of industrial biocatalytic processes (1.0 CFU);
- Biocatalytic reactor design and operation (0.50 CFU);
- Lab practice (1.00 CFU)
Informazioni sul programma
- Introduction to biocatalysis: why? how? The myth and the reality (0.5 CFU);
- Biocatalysis: how enzymes work (0.5 CFU);
- Biocatalysis at work: biotransformation of feedstocks into added-value molecules (0.5 CFU);
- Enzyme discovery and high throughput fermentation (0.5 CFU);
- Biocatalyst preparation (0.5 CFU):
- whole cells or isolated enzymes?
- immobilization;
- Techniques in biocatalysis: how biocatalysts can be improved? (1.0 CFU);
- protein engineering, directed evolution
- metabolic engineering
- Examples of industrial biocatalytic processes (1.0 CFU);
- Biocatalytic reactor design and operation (0.50 CFU);
- Lab practice (1.00 CFU)
Propedeuticità
Structure and functions of biomolecules
Fermentation biotechnology
Prerequisiti e modalità di esame
The course should be attended by students after the first semester, when courses concerning molecular methodologies, fermentation, and structure and function of biomolecules are given.
You can contact the teacher to obtain indications about texts and bibliographic references that can be studied to fill eventual knowledge gaps.
The student will be assessed through an oral presentation of a case-study chosen and selected with the teacher and after a discussion (20 minutes) which will encompass different issues and topics discussed during the course. You can get feedback on all practice and formal assessments so you can improve in the future.
Metodi didattici
Lessons (5 CFU)
Laboratory (1 CFU)
STUDENTI NON FREQUENTANTI
Programma
- Introduction to biocatalysis: why? how? The myth and the reality (0.5 CFU);
- Biocatalysis: how enzymes work (0.5 CFU);
- Biocatalysis at work: biotransformation of feedstocks into added-value molecules (0.5 CFU);
- Enzyme discovery and high throughput fermentation (0.5 CFU);
- Biocatalyst preparation (0.5 CFU):
- whole cells or isolated enzymes?
- immobilization;
- Techniques in biocatalysis: how biocatalysts can be improved? (1.0 CFU);
- protein engineering, directed evolution
- metabolic engineering
- Examples of industrial biocatalytic processes (1.0 CFU);
- Biocatalytic reactor design and operation (0.50 CFU);
- Lab practice (1.00 CFU)
Prerequisiti e modalità di esame
Course prerequisites and exam arrangements are the same for students attending and not attending the course lessons. Lesson attendance is nevertheless strongly encouraged
Periodo
Secondo semestre
Periodo
Secondo semestre
Modalità di valutazione
Esame
Giudizio di valutazione
voto verbalizzato in trentesimi
Docente/i
Ricevimento:
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