Molecular biochemistry and functional biology

A.A. 2019/2020
Insegnamento per
10
Crediti massimi
88
Ore totali
SSD
BIO/10 BIO/13
Lingua
Inglese

Struttura insegnamento e programma

Edizione attiva
Moduli o unità didattiche
Functional biology: from development to disease
BIO/10 - BIOCHIMICA - CFU: 0
BIO/13 - BIOLOGIA APPLICATA - CFU: 0
Esercitazioni di laboratorio a posto singolo: 16 ore
Lezioni: 32 ore

Molecular biochemistry
BIO/10 - BIOCHIMICA - CFU: 0
BIO/13 - BIOLOGIA APPLICATA - CFU: 0
Lezioni: 40 ore

STUDENTI FREQUENTANTI
Prerequisiti e modalità di esame
- Knowledge in biochemistry, cell biology, molecular biology, genetics is considered essential to optimize the comprehension of the topics discussed in the course.
- The final exam will be an essay followed by oral questions aimed at assessing the capacity of analyzing and critically discussing topics pertinent to the course.
Metodi didattici
Lectures and seminars given by experts. Mandatory practical demonstrations in the lab
Molecular biochemistry
Programma
The course will cover the following topics
1. Molecular mechanisms of regulation
- Principles of signal transduction
- Mechanisms of gene transcription
· Transcription factors
· Nuclear receptors
· Coregulators
- Transcriptional mechanisms in the regulation of metabolism
- Epigenetics
· Definition and basic principles
· Metabolism and epigenetics
· Epigenetics and differentiation
· Epigenetics in aging and disease

2. Molecular mechanisms underlying the metabolic functions of organs and tissues
Metabolic pathways and their regulation in different organs
- Glycolysis
- Gluconeogenesis
- Glycogen metabolism
- Pentose phosphate pathway
- Fatty acid metabolism
- Cholesterol metabolism
- Amino acid metabolism

3. Molecular aspects of metabolic pathways and functions in special cell types
- Metabolic pathways and their regulation in:
· Cancer cells
· Stem cells
· Cells of immune system
Metodi didattici
Lectures
Materiale didattico e bibliografia
pdf of the slides.
Harper, Review of Biochemistry, 24th edition
Nelson, Cox, Lehninger Principles of Biochemistry, 7th edition, W.H. Freeman and Company
Functional biology: from development to disease
Programma
The Course will cover the following subjects:
Introduction to Developmental Biology
Experimental approaches and techniques employed to study developmental mechanisms
Cellular and molecular mechanisms implicated in developmental biology
Animal models to study development (the big 6): Zebrafish, Drosophila Melanogaster, C. Elegans, Mus Musculus.
The fertilisation process
The first stages of development (gastrulation of fomration of the germinative layers)
Organogenesis: the nervous system, the neural crest cells, the heart, the muscles.
Techniques of mouse transgenesis (knockout, conditional, genome-editing based)
Metodi didattici
Lectures as well as seminars given by experts. In addition the students will attend mandatory practical demonstration in the lab
Materiale didattico e bibliografia
Slides as well as two main books:
- Slack: Essential Developmental Biology
- Wolpert: Developmental Biology
STUDENTI NON FREQUENTANTI
Prerequisiti e modalità di esame
- Knowledge in biochemistry, cell biology, molecular biology, genetics is considered essential to optimize the comprehension of the topics discussed in the course.
- The final exam will be an essay followed by oral questions aimed at assessing the capacity of analyzing and critically discussing topics pertinent to the course.
Molecular biochemistry
Programma
The course will cover the following topics
1. Molecular mechanisms of regulation
- Principles of signal transduction
- Mechanisms of gene transcription
· Transcription factors
· Nuclear receptors
· Coregulators
- Transcriptional mechanisms in the regulation of metabolism
- Epigenetics
· Definition and basic principles
· Metabolism and epigenetics
· Epigenetics and differentiation
· Epigenetics in aging and disease

2. Molecular mechanisms underlying the metabolic functions of organs and tissues
Metabolic pathways and their regulation in different organs
- Glycolysis
- Gluconeogenesis
- Glycogen metabolism
- Pentose phosphate pathway
- Fatty acid metabolism
- Cholesterol metabolism
- Amino acid metabolism

3. Molecular aspects of metabolic pathways and functions in special cell types
- Metabolic pathways and their regulation in:
· Cancer cells
· Stem cells
· Cells of immune system
Materiale didattico e bibliografia
pdf of the slides
Harper, Review of Biochemistry, 24th edition
Nelson, Cox, Lehninger Principles of Biochemistry, 7th edition, W.H. Freeman and Company
Functional biology: from development to disease
Programma
The Course will cover the following subjects:
Introduction to Developmental Biology
Experimental approaches and techniques employed to study developmental mechanisms
Cellular and molecular mechanisms implicated in developmental biology
Animal models to study development (the big 6): Zebrafish, Drosophila Melanogaster, C. Elegans, Mus Musculus.
The fertilisation process
The first stages of development (gastrulation of fomration of the germinative layers)
the organogenesis :the nervous system, the neural crest cells, the heart, the muscles.
Techniques of mouse transgenesis (knockout, conditional, genome-editing based)
Materiale didattico e bibliografia
Slides as well as two main books:
- Slack: Essential Developmental Biology
- Wolpert: Developmental Biology
Periodo
Primo semestre
Periodo
Primo semestre
Modalità di valutazione
Esame
Giudizio di valutazione
voto verbalizzato in trentesimi
Docente/i
Ricevimento:
su appuntamento
Via Balzaretti, 9
Ricevimento:
Venerdì mattina dalle 10:00 alle 12:00, previo appuntamento
Dip. di Scienze Farmacologiche e Biomolecolari, via Balzaretti 9
Ricevimento:
In giorni e orari concordati con lo studente interessato, previo appuntamento telefonico o attraverso posta elettronica
ufficio del docente presso il Dipartimento di Scienze Farmacologiche e Biomolecolari (Via Balzaretti)