Functional genomics and the molecular basis of differentiation
A.A. 2024/2025
Obiettivi formativi
The objective of the course is to provide students with: 1) a general overview on the principal methods for the study of bacterial genomes (genome sequencing techniques, genome annotation, phylogenetic studies); 2) a general overview on metagenomics and meta-trancriptomics analyses; 3) a practical approach for gene analyses, sequences alignment and comparison, use of the principal data bases, phylogenetic reconstructions.
In addition, the course provides students with information and training in the advances in the understanding of the molecular basis of genomic and epigenetic mechanisms in gene expression in livestock species. In detail the objective of the course is to provide students with 1) a general overview of the OMIC technologies used to study the primary structure of DNA, 2) detailed knowledge of the use of OMIC technologies in epigenetics 3) a general overview of epigenetics and role of epigenetic changes in development and cell differentiation
In addition, the course provides students with information and training in the advances in the understanding of the molecular basis of genomic and epigenetic mechanisms in gene expression in livestock species. In detail the objective of the course is to provide students with 1) a general overview of the OMIC technologies used to study the primary structure of DNA, 2) detailed knowledge of the use of OMIC technologies in epigenetics 3) a general overview of epigenetics and role of epigenetic changes in development and cell differentiation
Risultati apprendimento attesi
At the end of the course, the student will be able to plan experiments for the study of microbial communities, genomes sequencing and annotation and phylogenetic relationship between organisms. The practical approach of the course will introduce the student to bioinformatics with the use of tools capable to study genomes and the interaction between organisms.
In addition, the students will acquire knowledge on epigenetics changes on DNA and their effect on gene regulation and cell differentiation. Furthermore, the students will have a detailed overview on the new techniques applied to epigenetic profiling and acquire expertise on ENCODE database.
In addition, the students will acquire knowledge on epigenetics changes on DNA and their effect on gene regulation and cell differentiation. Furthermore, the students will have a detailed overview on the new techniques applied to epigenetic profiling and acquire expertise on ENCODE database.
Periodo: Primo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento non può essere seguito come corso singolo. Puoi trovare gli insegnamenti disponibili consultando il catalogo corsi singoli.
Programma e organizzazione didattica
Edizione unica
Responsabile
Periodo
Primo semestre
Programma
Genomics and molecular basis of differentiation in model invertebrate organisms and pathogens
1) An overview on the principal molecular biology methods
2) Sanger sequencing
3) A general overview on the principal methods for the study of bacterial genomes (genome sequencing techniques, genome annotation, phylogenetic studies);
4) a general overview on metagenomics and meta-trancriptomics analyses;
5) a practical approach to bioinformatics tools.
Genomics and epigenetics in vertebrates
Introduction to OMIC technologies in Livestock
Fundamentals of DNA, Chromosomes, Genes in Cell Division and Cell Cycle
Fundamentals of Gene Structure, Gene Expression: RNA genes and Non coding RNA
Next Generation sequencing applied DNA and RNA
Principles of Genetic Variation: functional genetic variation and protein polymorphism
Approaches to Mapping and Identifying Genetic Susceptibility to Complex traits
Epigenetic Theory
Epigenetic modifications; Methylation, Acetylation and Chromatin remodelling.
Principles of Gene Regulation and Epigenetics
Chromatin Modification and Epigenetic Factors in Gene Regulation
Epigenetics of Mammalian gamete and embryo development
Epigenetics and Animal Health
Epigenetics and MicroRNAs in livestock
1) An overview on the principal molecular biology methods
2) Sanger sequencing
3) A general overview on the principal methods for the study of bacterial genomes (genome sequencing techniques, genome annotation, phylogenetic studies);
4) a general overview on metagenomics and meta-trancriptomics analyses;
5) a practical approach to bioinformatics tools.
Genomics and epigenetics in vertebrates
Introduction to OMIC technologies in Livestock
Fundamentals of DNA, Chromosomes, Genes in Cell Division and Cell Cycle
Fundamentals of Gene Structure, Gene Expression: RNA genes and Non coding RNA
Next Generation sequencing applied DNA and RNA
Principles of Genetic Variation: functional genetic variation and protein polymorphism
Approaches to Mapping and Identifying Genetic Susceptibility to Complex traits
Epigenetic Theory
Epigenetic modifications; Methylation, Acetylation and Chromatin remodelling.
Principles of Gene Regulation and Epigenetics
Chromatin Modification and Epigenetic Factors in Gene Regulation
Epigenetics of Mammalian gamete and embryo development
Epigenetics and Animal Health
Epigenetics and MicroRNAs in livestock
Prerequisiti
Knowledge required for the access to the Degree Course.
Metodi didattici
Genomics and molecular basis of differentiation in model invertebrate organisms and pathogens
Lectures, practical laboratory activities, group work.
Genomics and epigenetics in vertebrates
Lectures and exercises
Journal clubs are organized on scientific papers as individual or group work. Exercises on the topics covered in the theoretical part.
Lectures, practical laboratory activities, group work.
Genomics and epigenetics in vertebrates
Lectures and exercises
Journal clubs are organized on scientific papers as individual or group work. Exercises on the topics covered in the theoretical part.
Materiale di riferimento
Genomics and molecular basis of differentiation in model invertebrate organisms and pathogens
Material provided by the teacher and uploaded to the MyAriel platform.
Genomics and epigenetics in vertebrates
1) Genetics and Genomics in Medicine , Tom Strachan, Judith Goodship, and Patrick Chinnery. Garland Science.
2) Articles provided during the lectures.
Material provided by the teacher and uploaded to the MyAriel platform.
Genomics and epigenetics in vertebrates
1) Genetics and Genomics in Medicine , Tom Strachan, Judith Goodship, and Patrick Chinnery. Garland Science.
2) Articles provided during the lectures.
Modalità di verifica dell’apprendimento e criteri di valutazione
Genomics and molecular basis of differentiation in model invertebrate organisms and pathogens
The learning assessment will be carried out through a written test: 20 multiple choice questions
Genomics and epigenetics in vertebrates
Written test. Exercises and open questions. The test will be graded out of thirty and the final grade will take into account the accuracy and quality of the answers
The learning assessment will be carried out through a written test: 20 multiple choice questions
Genomics and epigenetics in vertebrates
Written test. Exercises and open questions. The test will be graded out of thirty and the final grade will take into account the accuracy and quality of the answers
AGR/17 - ZOOTECNICA GENERALE E MIGLIORAMENTO GENETICO - CFU: 4
VET/06 - PARASSITOLOGIA E MALATTIE PARASSITARIE DEGLI ANIMALI - CFU: 4
VET/06 - PARASSITOLOGIA E MALATTIE PARASSITARIE DEGLI ANIMALI - CFU: 4
Esercitazioni: 36 ore
Lezioni: 30 ore
Lezioni: 30 ore
Docente/i
Ricevimento:
Tutti i giorni su appuntamento
Dipartimento di Medicina Veterinaria e Scienze Animali
Ricevimento:
Disponibilità previo appuntamento tramite e-mail
Dipartimento di Medicina Veterinaria e Scienze Animali (DIVAS)