Molecular Biology
A.Y. 2024/2025
Learning objectives
The aim of this course is to provide the students of a solid background in Molecular biology. Beside the description of basic processes such as DNA replication and repair, transcription, translation, a part of the course will be devoted to examples of regulation of these processes.
In addition, the students will acquaint with the principles of basic molecular biology techniques and basic knowledge on genome-wide approaches and post-genomic analyses that will be further developed in future courses to prepare for professional development in advanced biotechnology.
In addition, the students will acquaint with the principles of basic molecular biology techniques and basic knowledge on genome-wide approaches and post-genomic analyses that will be further developed in future courses to prepare for professional development in advanced biotechnology.
Expected learning outcomes
At the end of the course, the student will acquire the following skills:
- Understanding the complexity of the information flux from genes to proteins.
- Ability to comprehend and interpret data related to biomolecular processes investigated with large-scale approaches.
- Development of an aptitude to deal with the continuous advancements of molecular biology, a discipline in continuous expansion
- Ability to transfer the acquired molecular biology knowledge to related problems in the frame of the multiple biotechnology applications.
- Understanding the complexity of the information flux from genes to proteins.
- Ability to comprehend and interpret data related to biomolecular processes investigated with large-scale approaches.
- Development of an aptitude to deal with the continuous advancements of molecular biology, a discipline in continuous expansion
- Ability to transfer the acquired molecular biology knowledge to related problems in the frame of the multiple biotechnology applications.
Lesson period: First semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Linea AK
Responsible
Lesson period
First semester
Course syllabus
- History of molecular biology. Chemical bonds.
- Nucleic acids. DNA structure. Physico-chemical properties of DNA topology.
- Nucleic acids. Structure of the RNA.
- Genetic code and organization of the eukaryotic genome. Packaging of the human genome.
- The structure of proteins, interaction of proteins with nucleic acids.
- DNA replication.
- DNA damage repair.
- DNA recombination.
- The transcription in prokaryotes and eukaryotes
- The regulation of transcription in eukaryotes.
- Epigenetic regulation of transcription.
- 3D organization of the genome.
- RNA maturation.
- RNA splicing and outline of editing.
- Translation, translation and translation regulation apparatus.
- Outline of bioinformatics.
Techniques:
- Basic techniques for nucleic acids
- DNA sequencing techniques
- Technologies for the study of epigenetic regulation
- CRISPR-cas9 based techniques
- Techniques for the study of proteins
- Nucleic acids. DNA structure. Physico-chemical properties of DNA topology.
- Nucleic acids. Structure of the RNA.
- Genetic code and organization of the eukaryotic genome. Packaging of the human genome.
- The structure of proteins, interaction of proteins with nucleic acids.
- DNA replication.
- DNA damage repair.
- DNA recombination.
- The transcription in prokaryotes and eukaryotes
- The regulation of transcription in eukaryotes.
- Epigenetic regulation of transcription.
- 3D organization of the genome.
- RNA maturation.
- RNA splicing and outline of editing.
- Translation, translation and translation regulation apparatus.
- Outline of bioinformatics.
Techniques:
- Basic techniques for nucleic acids
- DNA sequencing techniques
- Technologies for the study of epigenetic regulation
- CRISPR-cas9 based techniques
- Techniques for the study of proteins
Prerequisites for admission
The course aims to provide solid basic knowledge of Molecular Biology. We will deal with the mechanisms of transcription, transcription regulation, translation, DNA replication and repair, principles of nucleic acids and proteins. Another objective of the course is the acquisition of general principles on the main molecular biology methodologies, some advanced techniques of DNA sequencing, genomic and post-genomic analyzes to provide the basis for subsequent specialized studies required for inclusion in professional contexts related to advanced biotechnology. Particular attention will also be given to hints of bioinformatics principles.
Teaching methods
Lectures with slide projection, videos or connection to web sites of database with attempts to interact with the class and to stimulate questions and curiosity toward the dealt topics.
Teaching Resources
Amaldi-Benedetti-Pesole-Plevani, Biologia Molecolare-terza edizione CEA
The slides of the course and other information will be available on the Portal ARIEL of University of Milan.
The slides of the course and other information will be available on the Portal ARIEL of University of Milan.
Assessment methods and Criteria
At the end of the course the student will register to one of the exam sessions. The exam is written and consists of a combination of problems and open questions. In case of need, the written work could also be supplemented with an oral examination.The final exam mark is the sum of the scores earned for each answer.
BIO/11 - MOLECULAR BIOLOGY - University credits: 9
Lessons: 72 hours
Professor:
Bodega Beatrice
Shifts:
Turno
Professor:
Bodega BeatriceLinea LZ
Responsible
Lesson period
First semester
BIO/11 - MOLECULAR BIOLOGY - University credits: 9
Lessons: 72 hours
Professor:
Pellicioli Achille
Shifts:
Turno
Professor:
Pellicioli AchilleProfessor(s)
Reception:
Wednesday 10.30-12.30 and Friday 10.30-11.30
Office