Several aspects of membrane biophysics. The role of lipid and protein components. Membrane microdomains and lipid-protein interactions.
Structural and functional features of membrane proteins with respect to transmembrane domains and the presence of alpha helix and/or beta sheets. Structure-function relationships. Function determination via structural homology analysis. Functional domains: homo- and heteromultimers.
Membrane specializations: t-tubules, neuromuscolar junctions
Membrane excitability, the membrane as a RC circuit.
The multifunctions of membrane proteins. Channel-type behaviour of transport proteins. Protein translocation between the cell membrane and cytoplasm in different cellular conditions. Methods used for the characterization of membrane proteins.
Examples of membrane transports mediated by carrier proteins, ion channels, V-ATPases, P-ATPases, in animal and plant cells will be discussed.
Papers related to the topics described will be analyzed.
Students are expected to have basic knowledge of cellular physiology, biochemistry, molecular biology and membrane biology.
Interactive frontal lessons, where students are encouraged to attend and to actively participate. Assigned papers to be discussed by students in small groups or singularly. Presented materials will be made available through the ARIEL website.