Functional Genomics is the field of molecular biology that is involved with assigning function and interactions to genes and their products and ranges from the detailed study of individual genes (forward genetics), large scale experimental approaches designed to capture global effects and interactions. The course in Functional Genomics" provides participants with deep knowledge about the latest genome wide and single gene approaches to study gene functions in model organisms. Teaching is especially focused on helping students gain the expertise required to decide which approaches and technologies to use to investigate biological pathways and the function of genes involved in them.
The development of several high throughput analytical approaches in molecular biology has revolutionized genomics. In particular, Next Generation Sequencing has wide application to many functional genomics settings.
Risultati apprendimento attesi
Periodo: Primo semestre
(se vi sono più edizioni controllare anche il periodo dell'edizione, che può essere diverso)
The course is divided in three biological levels of gene function analysis: (i) the DNA level: this part focuses on the history of DNA sequencing from the start to the latest technologies and allows choosing of the right technology for different application. Furthermore, technologies to generate mutants in model organisms will be discussed. (ii) the RNA level: this part focuses on transcriptome analysis and how these analyses are used to understand gene functions. (iii). The protein level: identification and analysis of the proteome, analysis of protein complexes and trafficking to understand gene functions.
To pass the examination students will have to follow the lessons since self-study is difficult due to the absence of a text book that covers the course. The exam consists of 6 open questions of which the student selects 5 to answer. The examination is in English.
Frontal teaching with a high level of teacher interaction supported by projected teaching material which is available to students from a dedicated website. Extensive discussions to allow development of critical faculties and encourage constructive individual involvement in the teaching/learning process.
Materiale di riferimento
This course is not covered by a text book since the latest technologies will be discussed. Literature will be provided during the lessons and through the website.