Molecular virology

A.A. 2019/2020
8
Crediti massimi
66
Ore totali
SSD
VET/03 VET/05
Lingua
Inglese
Obiettivi formativi
Provide deep molecular knowledge in the field of veterinary virology, driving the students to understand the mechanisms of virus replication, the interaction with cells in which they replicate, the diagnosis, the pathogenesis and the strategies to control viral diseases, including zoonosis. Laboratory practices aim to grow, isolate and characterize animal viruses.
Risultati apprendimento attesi
The student is expected to reach the following results:
     Theoretical learning:
basic veterinary molecular virology
background of the main viral diseases transmitted to human from animals, and molecular and biochemical changes related to these pathologies
main techniques applied in laboratories of virology and molecular diagnostics
    Practical learning:
application of in vitro methods to grow and isolate viruses
assessment of cytopathic effects due to different vituses on tissue culture
purification of viruses
application of molecular biology techniques to assess major biochemical and molecular modifications associated to viral diseases,
measurement of mRNA abundance expression and make judgements on its relationship with the effect of virus infection
assessment of the quality of a blood smear, and provide an interpretation of the results of a quantitative PCR base molecular assay.
    Communication:
understand and communicate using the proper terms related to Veterinary Virology and Viral Pathology  
Corso singolo

Questo insegnamento non può essere seguito come corso singolo. Puoi trovare gli insegnamenti disponibili consultando il catalogo corsi singoli.

Programma e organizzazione didattica

Edizione unica

Responsabile
Periodo
Primo semestre

STUDENTI FREQUENTANTI
Prerequisiti
Oral and written exam
Metodi didattici
Theoretical and practical
Unita' didattica: Molecular Basis of Virology
Programma
Theoretical classes:
Structure and composition of animal viruses
In vitro and in vivo characteristics of viral infections
Methods to study and to purify viruses
Diagnostic virology
How to grow viruses: animals, embryonated eggs and cell cultures
Anti-viral immune response
Anti-viral therapy
Viruses in gene therapy

Laboratory classes:
In vitro culture and splitting of a cell line
Cell count and procedure to freeze cell lines
In vitro infection
Cytopatic effects of different viruses on cell lines
Virus purification
Metodi didattici
Theoretical and practical classes
Materiale di riferimento
Handouts will be provided by the teacher
Unita' didattica: Molecular pathogenesis of viral diseases
Programma
Lectures:
1. Viral pathogenesis
2. Immune Response to Viral Infections
3. SARS Corona viruses
4. Filoviruses : Ebola and Marburg
5. HIV
6. Influenza viruses

Seminars:
1. Emerging viral diseases
2. Herpes viruses

Practices
- Infection of bovine monocytes with BVD virus. The students will carry out an experimental infection of bovine monocytes with the virus of BVD. Bovine will be purified and infected with viruses. Cytopathic effects will be identified, and the expression of proteins involved in immune reaction will be assessed
- Histopathology of viral infections
Metodi didattici
Theoretical and practical classes
Materiale di riferimento
Handouts and pdf of the classes are available via ARIEL platform
STUDENTI NON FREQUENTANTI
Prerequisiti
Oral and written exam
Unita' didattica: Molecular Basis of Virology
Programma
Theoretical classes:
Structure and composition of animal viruses
In vitro and in vivo characteristics of viral infections
Methods to study and to purify viruses
Diagnostic virology
How to grow viruses: animals, embryonated eggs and cell cultures
Anti-viral immune response
Anti-viral therapy
Viruses in gene therapy

Laboratory classes:
In vitro culture and splitting of a cell line
Cell count and procedure to freeze cell lines
In vitro infection
Cytopatic effects of different viruses on cell lines
Virus purification
Materiale di riferimento
Handouts will be provided by the teacher
Unita' didattica: Molecular pathogenesis of viral diseases
Programma
Lectures:
1. Viral pathogenesis
2. Immune Response to Viral Infections
3. SARS Corona viruses
4. Filoviruses : Ebola and Marburg
5. HIV
6. Influenza viruses

Seminars:
1. Emerging viral diseases
2. Herpes viruses

PRACTICES
Scientific papers focused on the topics practiced during the course will be uploaded on the ARIEL platform. The student will be asked to deliver a short writtne presentation of the papers during the exam.
Materiale di riferimento
Handouts and pdf of the classes are available via ARIEL platform
Moduli o unità didattiche
Unita' didattica: Molecular Basis of Virology
VET/03 - PATOLOGIA GENERALE E ANATOMIA PATOLOGICA VETERINARIA
VET/05 - MALATTIE INFETTIVE DEGLI ANIMALI DOMESTICI
Esercitazioni: 24 ore
Lezioni: 18 ore
Docente: Turin Lauretta
Turni:
-
Docente: Turin Lauretta

Unita' didattica: Molecular pathogenesis of viral diseases
VET/03 - PATOLOGIA GENERALE E ANATOMIA PATOLOGICA VETERINARIA
VET/05 - MALATTIE INFETTIVE DEGLI ANIMALI DOMESTICI
Esercitazioni: 12 ore
Lezioni: 12 ore
Turni:
-
Docente: Ceciliani Fabrizio

Docente/i
Ricevimento:
Da concordare via mail
Dipartimento di Medicina Veterinaria e Scienze Animali- Stanza 172
Ricevimento:
Lunedi 9-13
Polo di Lodi