- The genome organization in eukaryotic cells
- Non-coding RNAs
- Epigenetic regulation of gene expression (DNA methylation, histone variants and modifications, chromatin accessibility and ncRNAs association, etc), technologies and alterations in human diseases
- Tridimensional organization of the genome (polymer folding principles, TADs and long-range interactions), technologies and alterations in human diseases
- Genome regulation through compartmentalization and phase separation (nucleolus, transcription factories and repression bodies, LADs, nuclear pore, etc), technologies and alterations in human diseases
- Models for studying genome organization (CRISPRs/cas9 cell systems, organoids, mouse models etc)
- Control of cell proliferation and growth
- The cell cycle control systems (S phase, mitosis, completion of mitosis and cell division)
- DNA repair and DNA damage tolerance mechanisms
- Single cell analysis of transcription and genome structure
- Single molecule tracking to study transcription factors consensus site recognition and other proteins involved in DNA metabolism
- Super-resolution imaging approaches to study the localization of transcription factors, repair protein and other DNA metabolic factors and accessible regions of the genome.
- Molecular genetics approaches to study protein/DNA, protein/protein and protein/RNA interactions at the single molecule resolution.
- In vivo imaging to study key DNA metabolic factors mediating cancer cell proliferation and metastasis
- Pathologies related to increased genome instability
- Cell response to environmental cues (infection and immune response, heat shock genes response, vernalization, mechanical stress, tumor microenvironment, etc).
The course will require basic knowledge of cell biology and biochemistry and a good knowledge of molecular biology and genetics. Students must be aware of the principles of the DNA metabolisms, notably transcription, replication, repair and recombination. They must have knowledege of concepts related to cell growth, cell cycle progression, cell differentiation, and basic knowledged of formation of organs and tissues. They must be aware of concepts related to multi-subunit protein complexes, and on the principles of enzymatic activities.
Traditional lectures supported by slides integrated by on-site interactive discussion sessions, in which student's involvement will be promoted through assignment of scientific papers in advance, with the purpose of stimulating group discussions and to develop students' critical and communication skills.
All the teaching materials will be available through the Ariel platform.
Regular attendance is strongly suggested.
Materiale di riferimento
The topics covering most of the course are very novel and as a consequence there is no comprehensive text. Web sites and review papers will be indicated to the students. Lectures will be recorded made available through the Teams and Ariel platforms, together all the materials instrumental to allow the students to participate to the on-site discussion lessons, such as original articles and reviews.