Functional genomics

A.A. 2020/2021
Crediti massimi
Ore totali
BIO/11 BIO/18
Obiettivi formativi
Now that most genomes of model organisms have been sequenced and annotated in detail, reverse genetics approaches are being used to experimentally determine the function of genes encoded by genomes. Most gene functions up until today are largely based on bioinformatics-based predictions. However, those that have been experimentally analysed, especially regulatory genes, reveal a complex picture of genes having multiple and often overlapping functions. In this complex framework, the course intends to provide the students with deep knowledge about the latest technologies and genome wide approaches to study gene functions in model organisms and to unravel complex regulatory pathways.
Risultati apprendimento attesi
Students will become familiar with approaches used to study gene functions using state-of-the-art genomics, transcriptomics and proteomics approaches. The course commences with genome analysis and reverse genetics approaches applied to study gene functions. Subsequently, transcriptomics and protein analysis will be used to study transcription factors that control regulatory pathways. Students will gain competences in understanding the different methodologies used for gene function analysis in complex biological systems.
BY the end of the course, students will be able to design experimental strategies to study gene functions in prokaryotic and eukaryotic model organisms.
Programma e organizzazione didattica

Edizione unica

Primo semestre
Lectures are provided via Ariel asynchronously by power point presentations with audio files. Regularly lessons (total 12 CFU) will take place via the Zoom platform to help and facilitate a better understanding of the lessons.

Evaluation will be through written exams (online) using the / SEB protocol and Zoom surveillance (see instruction manual on Ariel). The examination consists of 6 open questions of which the students will have to select 5 questions to answer.
The content and other considerations are all unchanged.
Whole genome sequencing
Genome annotation
Gene family analysis
Reverse genetics strategies in prokaryotes and eukaryotes
Small non-coding RNA function and their analysis
Regulatory pathway analysis
Proteomics strategies
Protein interactions and complex formation
Protein analysis in living cells.
Good basic knowledge of Molecular Biology and Genetics.
Metodi didattici
Frontal lectures. At the end of the course, there will be an additional question time during which students can ask for extra explanations regarding all the topics that were discussed during the lessons.
Materiale di riferimento
Scientific papers and reviews will be presented during lectures and all materials will be uploaded on the Ariel website, lecture attendance is strongly encouraged.
Modalità di verifica dell’apprendimento e criteri di valutazione
Learning assessment will be through a written exam at the end of the course. The examination will consist of 6 open questions of which, students must answer 5. Each question contributes equally to the final score.
Lezioni: 48 ore
Siti didattici
Mercoledì 15:00-17:00
Secondo piano torre B
Giovedi 14.00 - 17.00
Via Celoria 26, Torre B, Piano 2
Appointments on request by email