Molecular microbiology and genetics of microorganisms
A.A. 2020/2021
Obiettivi formativi
The aims of the course are to illustrate:
i) how bacteria sense different environmental stimuli;
ii) how gene expression is regulated in bacteria at the level of single cells and populations;
iii) available methods and strategies in bacterial research.
i) how bacteria sense different environmental stimuli;
ii) how gene expression is regulated in bacteria at the level of single cells and populations;
iii) available methods and strategies in bacterial research.
Risultati apprendimento attesi
At the end of the course, students will reach an integrated view of the mechanisms by which bacterial cells perceive environmental stimuli, transduce them as intracellular signals and implement proper regulatory and physiological responses, and they will be able to critically integrate new knowledge in this context. Moreover, students will be prepared to propose and clearly communicate strategies and experimental approaches to the resolution of biological problems.
Periodo: Primo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento non può essere seguito come corso singolo. Puoi trovare gli insegnamenti disponibili consultando il catalogo corsi singoli.
Programma e organizzazione didattica
Edizione unica
Responsabile
Periodo
Primo semestre
Teaching methods: Learning activities will be provided through a combination of asynchronous and synchronous lessons. In the 24 hours preceding each scheduled lesson, a video lesson will be made available on the Ariel pages of the course, consisting of the recording of the teacher's desktop with synthetic audio commentary. In addition, in correspondence with the scheduled lessons, the teacher will be available in chat on the Microsoft Teams platform for clarifications regarding the content of the lessons. Approaches to the resolution of biological problems applying the notions provided in the video-lectures will be illustrated in synchronous lessons on Microsoft Teams, also exploiting poll systems (e.g Slido) to assess students' comprehension of the discussed topics. Synchronous lessons will be recorded and uploaded on Ariel course site for students unable to follow them. The dates scheduled for the synchronous lectures will be published on time in the Ariel pages of the course, as well as the notices relating to any update related to the evolution of the rules imposed by Covid-19.
Reference materials: The program and the reference material will not change.
Verification of learning and evaluation criteria: The remote exams that may be imposed by the Covid-19 emergency will be conducted entirely orally on the Microsoft Teams platform and will consist of 4-5 questions about the scientific topics discussed during the lessons.
Reference materials: The program and the reference material will not change.
Verification of learning and evaluation criteria: The remote exams that may be imposed by the Covid-19 emergency will be conducted entirely orally on the Microsoft Teams platform and will consist of 4-5 questions about the scientific topics discussed during the lessons.
Programma
-Introduction to general strategies of gene expression regulation in bacteria: sigma and anti-sigma factors, repressors and activators.
-Regulation of the arabinose operon.
-Response to genotoxic agents: the SOS response and spread of antibiotic resistance. Bacterial persisters.
-Response to temperature upshift: RpoH and RpoE regulons.
-Regulation by non-coding RNA
-Signal transduction by Two-Component Regulatory Systems (TCRS). Bacterial chemotaxis.
-Cell-to-cell communication in microbial communities. Quorum sensing and biofilm formation.
-Bacteria-Eukarya relationship. General concepts and methods to the study of virulence and virulence genes
-Regulation of the arabinose operon.
-Response to genotoxic agents: the SOS response and spread of antibiotic resistance. Bacterial persisters.
-Response to temperature upshift: RpoH and RpoE regulons.
-Regulation by non-coding RNA
-Signal transduction by Two-Component Regulatory Systems (TCRS). Bacterial chemotaxis.
-Cell-to-cell communication in microbial communities. Quorum sensing and biofilm formation.
-Bacteria-Eukarya relationship. General concepts and methods to the study of virulence and virulence genes
Prerequisiti
The students must have basic knowledge (Bachelor level) in Microbiology, Genetics and Molecular biology.
Metodi didattici
Traditional, Powerpoint-assisted lectures, with ample time dedicated to biological problem solving and the discussion of different approaches to answer biological questions. Students are strongly encouraged to attend lessons and take part in the active discussions. It is strongly recommended that students attend lectures.
Materiale di riferimento
General concepts in Microbiology can be retrieved in: Brock Biology of Microorganisms (15th Edition) by M. Madigan et al. Specific review articles on the topics treated during the classes will be proposed. The slides shown during the classes will be available on the course Ariel website.
Modalità di verifica dell’apprendimento e criteri di valutazione
Learning assessments will be carried out by a written exam at the end of the course.
The exam includes one open question (50% of the final score), and multiple choices and/or schematic questions (50% of the final score). Multiple choice questions are aimed to verify the global understanding of the key concepts and definitions taught during the course, whereas the open question is designed to evaluate the ability of the student to apply the concepts and experimental approaches taught in the course to the resolution of simple biological problems concerning the processes and regulatory mechanisms discussed in class.
The exam includes one open question (50% of the final score), and multiple choices and/or schematic questions (50% of the final score). Multiple choice questions are aimed to verify the global understanding of the key concepts and definitions taught during the course, whereas the open question is designed to evaluate the ability of the student to apply the concepts and experimental approaches taught in the course to the resolution of simple biological problems concerning the processes and regulatory mechanisms discussed in class.
Docente/i
Ricevimento:
mercoledì 15:30-16:30 previo appuntamento richiesto per e-mail
DBS, via Celoria 26, IV piano, torre C