Omics: from bench to bedside
A.A. 2020/2021
Obiettivi formativi
The aim of the course is to present to the students the fundamental logic in the use of omic methodologies, to introduce the theoretical and practical bases and the principles on which the different omic techniques are based, to describe the main protocols of use both in the preparation of the sample and of the instrumentation and their control, and finally to provide the basis for the management and analysis of the results including the possibility of integration of the data obtained with different omic methodologies.
In addition, since omics-based recent approaches have unveiled the molecular mechanism behind tumorigenesis of several human tumors and promoted the identification of next-generation molecular markers for early diagnosis, prognosis and even personalized targeted therapy, the course will introduce the student to the most promising omics approaches and their outcomes for the diagnosis and therapy of cancer.
In addition, since omics-based recent approaches have unveiled the molecular mechanism behind tumorigenesis of several human tumors and promoted the identification of next-generation molecular markers for early diagnosis, prognosis and even personalized targeted therapy, the course will introduce the student to the most promising omics approaches and their outcomes for the diagnosis and therapy of cancer.
Risultati apprendimento attesi
At the end of the course, the students will be able to address different scientific issues and to choose and apply the best omics approaches to understand the biochemical bases of tissue functions in health and disease. The student will be prepared to discuss the available modern high technologies to identify novel tumor markers and understand personalized therapies; they will learn the "omics" work flow from different clinical matrices (i.e. tumor specimen) to data analysis.
Periodo: Secondo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento non può essere seguito come corso singolo. Puoi trovare gli insegnamenti disponibili consultando il catalogo corsi singoli.
Programma e organizzazione didattica
Edizione unica
Periodo
Secondo semestre
Se la situazione lo consente, le lezioni si terranno in aula. Ogni lezione sarà videoregistrata e resa disponibile in modo asincrono attraverso la piattaforma Ariel. Se la situazione peggiorasse o non ci fossero aule adatte a contenere tutti gli studenti del corso rispettando il distanziamento sociale, le lezioni verranno erogate tramite la piattaforma Microsoft Teams e potranno essere seguite in modo sincrono secondo l'orario programmato e in modo asincrono perché registrate tramite la stessa piattaforma.
Programma
The following topics will be covered during the section of Omics technology and approaches:
- Introduction to genomics
- Sequencing technology platform
- Single Nucleotide polymorphisms (SNPs), exome sequencing and genome-wide association study (GWAS)
- DNA-protein interaction at the genome level (chromatin immunoprecipitation coupled to next-generation sequencing-ChIP-Seq)
- Functional genomics screens
- Epigenomics
- Metagenomics
- Introduction to transcriptomics
- Nascent RNA and total RNA sequencing
- Splicing analysis
- Other RNAs analysis (miRNAs, long non-coding RNAs, small nuclear RNAs, and others)
- Introduction to proteomics
- Proteomic platform and instrumentation (high resolution nanoLC-MS/MS system)
- Labelled and label-free proteomics
- Imaging proteomics and applications
- Structural proteomics
- Introduction to metabolomics
- Metabolic platform: targeted vs untargeted metabolomics
- Lipidomics
- Fluxomics
- Introduction to bioinformatics
- Omics data integration
- Principle of system biology and application to disease.
The following topics will be covered during the section of "Omics approaches in oncodiagnostics and therapy"
- Elements of oncogenic transformation, tumor classification, and phenotype, epidemiology and risk factors of most common neoplasia. Hereditary cancers
- Omics-based approaches to investigate the molecular mechanism of cancer transformation
- Omics-based approaches to identify molecular markers for early diagnosis and prognosis of the most common tumors (from histology to liquid biopsy). Hereditary cancers.
- Cancer therapy and immunotherapy: examples of personalized treatments based on novel biotech drugs and/or treatments
- Introduction to genomics
- Sequencing technology platform
- Single Nucleotide polymorphisms (SNPs), exome sequencing and genome-wide association study (GWAS)
- DNA-protein interaction at the genome level (chromatin immunoprecipitation coupled to next-generation sequencing-ChIP-Seq)
- Functional genomics screens
- Epigenomics
- Metagenomics
- Introduction to transcriptomics
- Nascent RNA and total RNA sequencing
- Splicing analysis
- Other RNAs analysis (miRNAs, long non-coding RNAs, small nuclear RNAs, and others)
- Introduction to proteomics
- Proteomic platform and instrumentation (high resolution nanoLC-MS/MS system)
- Labelled and label-free proteomics
- Imaging proteomics and applications
- Structural proteomics
- Introduction to metabolomics
- Metabolic platform: targeted vs untargeted metabolomics
- Lipidomics
- Fluxomics
- Introduction to bioinformatics
- Omics data integration
- Principle of system biology and application to disease.
The following topics will be covered during the section of "Omics approaches in oncodiagnostics and therapy"
- Elements of oncogenic transformation, tumor classification, and phenotype, epidemiology and risk factors of most common neoplasia. Hereditary cancers
- Omics-based approaches to investigate the molecular mechanism of cancer transformation
- Omics-based approaches to identify molecular markers for early diagnosis and prognosis of the most common tumors (from histology to liquid biopsy). Hereditary cancers.
- Cancer therapy and immunotherapy: examples of personalized treatments based on novel biotech drugs and/or treatments
Prerequisiti
Conoscenze di base della Biochimica, della biochimica applicata e immunologia. Elementi di fisiopatologia umana sono fortemente raccomandati.
Metodi didattici
Il Corso è composto da due moduli: 1. Omics technology and approaches (32 h) and 2. Omics approaches in oncodiagnostics and therapy (16 h) di lezioni frontali, come precedentemente descritto, per un totale di 48 ore. Entrambi i moduli saranno tenuti dai Docenti e da esperti su particolari temi. Tutto il materiale è disponibile sul sito Ariel del Corso.
Materiale di riferimento
The iconographic material shown during the lessons and bibliographic references can be downloaded from the Ariel portal.
Modalità di verifica dell’apprendimento e criteri di valutazione
La preparazione degli studenti sarà verificata con un colloquio orale che verterà sulle tematiche trattate durante il corso
Moduli o unità didattiche
Omics approaches in oncodiagnostics and therapy
BIO/10 - BIOCHIMICA
MED/04 - PATOLOGIA GENERALE
MED/04 - PATOLOGIA GENERALE
Lezioni: 16 ore
Docenti:
D'Alessandro Sarah, Taramelli Donatella
Omics technology and approaches
BIO/10 - BIOCHIMICA
MED/04 - PATOLOGIA GENERALE
MED/04 - PATOLOGIA GENERALE
Lezioni: 32 ore
Docenti:
Caruso Donatella Maria, Mitro Nico
Docente/i
Ricevimento:
da concordare
via Carlo Pascal, 36 - 20133 MILANO
Ricevimento:
Su appuntamento telefonico o e-mail
Via Balzaretti 9, 20113 Milano
Ricevimento:
Previo appuntamento telefonico o E mail
Dipartimento di Scienze Farmacologiche e Biomolecolari- Sede di Via Pascal 36- I piano