Applied biocatalysis
A.A. 2021/2022
Obiettivi formativi
The course is aimed to provide to the students the basic knowledge necessary to develop a bioprocess based on the application of enzymes and/or microbial cells, which needs to gather tools across different biotechnological techniques. Specific objects of the course are the following:
- Learning how to apply the biotechnological techniques learned during the first year of the master degree for the evolution of enzymes or microbial cells into efficient biocatalysts;
- Learning from consolidated and/or more innovative and recent successful examples on how a biocatalytic process is developed and optimized;
- Assessing the most suited technologies for intensifying a biocatalytic process
- Learning how to apply the biotechnological techniques learned during the first year of the master degree for the evolution of enzymes or microbial cells into efficient biocatalysts;
- Learning from consolidated and/or more innovative and recent successful examples on how a biocatalytic process is developed and optimized;
- Assessing the most suited technologies for intensifying a biocatalytic process
Risultati apprendimento attesi
At the end of the course the student will know why biocatalyses is strategic for the bioeconomy in a bio-based industry context. The student will be familiar with the essential steps in the development of biocatalytic processes from concept to completion, by the application of the best suited methodologies (protein engineering, metabolic engineering, immobilization, continuous processes) to develop an innovative biocatalytic process of industrial interest. The focus will be on bioprocesses relevant in the agri-food and bioenergy fields.
Periodo: Secondo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento non può essere seguito come corso singolo. Puoi trovare gli insegnamenti disponibili consultando il catalogo corsi singoli.
Programma e organizzazione didattica
Edizione unica
Responsabile
Periodo
Secondo semestre
More specific information on the delivery modes of training activities for academic years 2021/2022 will be provided over the coming months, based on the evolution of the public health
Programma
- Introduction to biocatalysis: why? how? The myth and the reality (0.5 CFU);
- Biocatalysis: how enzymes work (0.5 CFU);
- Biocatalysis at work: biotransformation of feedstocks into added-value molecules (0.5 CFU);
- Enzyme discovery and high throughput fermentation (0.5 CFU);
- Biocatalyst preparation (0.5 CFU):
- whole cells or isolated enzymes?
- immobilization;
- Techniques in biocatalysis: how biocatalysts can be improved? (1.0 CFU);
- protein engineering, directed evolution
- metabolic engineering
- Examples of industrial biocatalytic processes (1.0 CFU);
- Biocatalytic reactor design and operation (0.50 CFU);
- Lab practice (1.00 CFU)
- Biocatalysis: how enzymes work (0.5 CFU);
- Biocatalysis at work: biotransformation of feedstocks into added-value molecules (0.5 CFU);
- Enzyme discovery and high throughput fermentation (0.5 CFU);
- Biocatalyst preparation (0.5 CFU):
- whole cells or isolated enzymes?
- immobilization;
- Techniques in biocatalysis: how biocatalysts can be improved? (1.0 CFU);
- protein engineering, directed evolution
- metabolic engineering
- Examples of industrial biocatalytic processes (1.0 CFU);
- Biocatalytic reactor design and operation (0.50 CFU);
- Lab practice (1.00 CFU)
Prerequisiti
The course should be attended by students after the first semester, when courses concerning molecular methodologies, fermentation, and structure and function of biomolecules are given.
You can contact the teacher to obtain indications about texts and bibliographic references that can be studied to fill eventual knowledge gaps.
You can contact the teacher to obtain indications about texts and bibliographic references that can be studied to fill eventual knowledge gaps.
Metodi didattici
The course is divided into lectures and practical laboratories
Materiale di riferimento
Notes and monographs made available by the teacher
Modalità di verifica dell’apprendimento e criteri di valutazione
The student will be assessed through an oral presentation of a case-study chosen and selected with the teacher and after a discussion (20 minutes) which will encompass different issues and topics discussed during the course. You can get feedback on all practice and formal assessments so you can improve in the future.
CHIM/11 - CHIMICA E BIOTECNOLOGIA DELLE FERMENTAZIONI - CFU: 6
Laboratori: 16 ore
Lezioni: 40 ore
Lezioni: 40 ore
Docente:
Molinari Francesco Enzo
Docente/i
Ricevimento:
previo appuntamento
via Luigi Mangiagalli 25, terzo piano