Functional genomics

A.A. 2021/2022
Crediti massimi
Ore totali
Obiettivi formativi
Now that most genomes of model organisms have been sequenced and annotated in detail, reverse genetics approaches are being used to experimentally determine the function of genes encoded by genomes. Most gene functions up until today are largely based on bioinformatics-based predictions. However, those that have been experimentally analysed, especially regulatory genes, reveal a complex picture of genes having multiple and often overlapping functions. In this complex framework, the course intends to provide the students with deep knowledge about the latest technologies and genome wide approaches to study gene functions in model organisms and to unravel complex regulatory pathways.
Risultati apprendimento attesi
Students will become familiar with approaches used to study gene functions using state-of-the-art genomics, transcriptomics and proteomics approaches. The course commences with genome analysis and reverse genetics approaches applied to study gene functions. Subsequently, transcriptomics and protein analysis will be used to study transcription factors that control regulatory pathways. Students will gain competences in understanding the different methodologies used for gene function analysis in complex biological systems.
BY the end of the course, students will be able to design experimental strategies to study gene functions in prokaryotic and eukaryotic model organisms.
Programma e organizzazione didattica

Edizione unica

Primo semestre
More specific information on the delivery modes of training activities for academic year 2021/22 will be provided over the coming months, based on the evolution of the public health situation
Whole genome sequencing
Genome annotation
Gene family analysis
Reverse genetics strategies in prokaryotes and eukaryotes
Small non-coding RNA function and their analysis
Regulatory pathway analysis
Proteomics strategies
Protein interactions and complex formation
Protein analysis in living cells.
Good basic knowledge of Molecular Biology and Genetics.
Metodi didattici
Frontal lectures. At the end of the course, there will be an additional question time during which students can ask for extra explanations regarding all the topics that were discussed during the lessons.
Materiale di riferimento
Scientific papers and reviews will be presented during lectures and all materials will be uploaded on the Ariel website, lecture attendance is strongly encouraged.
Modalità di verifica dell’apprendimento e criteri di valutazione
Learning assessment will be through a written exam at the end of the course. The examination will consist of 6 open questions of which, students must answer 5. Each question contributes equally to the final score.
Lectures: 48 ore
Giovedì(Thursday) 15:00-17:00
Secondo piano torre B
Giovedi 14.00 - 17.00
Via Celoria 26, Torre B, Piano 2
Appointments on request by email