Advanced molecular and cellular biotechnology
A.A. 2023/2024
Obiettivi formativi
The main objective of the course is to gain a deep cellular and molecular knowledge of structures and functions of cells and genomes. Innovative approaches and methodologies will be described, with direct implication for biotechnology. While we strive to keep overlaps with other courses to a minimum, our course touches several aspects of genome and cell biology, with specific reference to biotechnology applications, that are also discussed in structural biology, bioinformatics, and genome engineering courses.
Risultati apprendimento attesi
After attending the course, the students will be able to discuss a critical points, experimental designs and interpretation of the results about the topics presented., specifically:
- principles, origin and uses of tissue cultures;
- the use of engineered mice to study gene function in physiology and pathology;
- Drosophila melanogaster as a genetic model for disease;
- rudiment of cell cell signaling and its alterations in tumorigenesis;
- introductions to other experimental model systems (Zebrafish, organoids, biofabrications);
- data protection, ethics of science and research integrity;
- basic and advanced concepts of DNA damage response (DDR);
- basic and advanced concepts of recombination DNA repair;
- basic concepts of cancer therapy approaches, with specific reference to DDR.
- principles, origin and uses of tissue cultures;
- the use of engineered mice to study gene function in physiology and pathology;
- Drosophila melanogaster as a genetic model for disease;
- rudiment of cell cell signaling and its alterations in tumorigenesis;
- introductions to other experimental model systems (Zebrafish, organoids, biofabrications);
- data protection, ethics of science and research integrity;
- basic and advanced concepts of DNA damage response (DDR);
- basic and advanced concepts of recombination DNA repair;
- basic concepts of cancer therapy approaches, with specific reference to DDR.
Periodo: Secondo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento può essere seguito come corso singolo.
Programma e organizzazione didattica
Edizione unica
Responsabile
Periodo
Secondo semestre
Programma
The program will cover different molecular aspects of cell biology with implications for biotechnology.
Part A (5CFU; Prof. Vaccari and Prof. Biffo).
Prof. Vaccari: An Introduction to model system biology; How to connect cell biology data to tissue and organismal contexts; Genetic tools for cell and tissue biology: The case of Drosophila; Forward and reverse genetic approaches for understanding of gene function; Signal transduction biology: The case of Notch and membrane trafficking; In vivo modeling of monogenic diseases.
Prof. Biffo: Principles of cell culture; Established and primary cell lines. Other types of cell cultures: tissue slices and the development of 3D-models; Modern analytical methods. Concepts of differentiation and oncogenesis at work in cell culture.
Part B (5 CFU; Pellicioli). The problem of genome integrity maintenance. Specific arguments: keys factors and mechanisms of the DNA repair and DNA damage checkpoint pathways; DNA recombination mechanisms that drive genome rearrangements and instability; molecular perspective to understand cancer development and treatment; exploiting DNA damage response pathways for genome engineering.
Part A (5CFU; Prof. Vaccari and Prof. Biffo).
Prof. Vaccari: An Introduction to model system biology; How to connect cell biology data to tissue and organismal contexts; Genetic tools for cell and tissue biology: The case of Drosophila; Forward and reverse genetic approaches for understanding of gene function; Signal transduction biology: The case of Notch and membrane trafficking; In vivo modeling of monogenic diseases.
Prof. Biffo: Principles of cell culture; Established and primary cell lines. Other types of cell cultures: tissue slices and the development of 3D-models; Modern analytical methods. Concepts of differentiation and oncogenesis at work in cell culture.
Part B (5 CFU; Pellicioli). The problem of genome integrity maintenance. Specific arguments: keys factors and mechanisms of the DNA repair and DNA damage checkpoint pathways; DNA recombination mechanisms that drive genome rearrangements and instability; molecular perspective to understand cancer development and treatment; exploiting DNA damage response pathways for genome engineering.
Prerequisiti
Good knowledge of Molecular Biology, Genetics and Biochemistry is required.
Metodi didattici
Teaching mode: Traditional lectures supported by slides and more interactive lectures with open discussion on selected aspects (Journal and data club). Attendance is highly recommended.
Materiale di riferimento
Scientific papers and reviews will be indicated during the course and made available to students through the website (https://tvaccariamcb.ariel.ctu.unimi.it). The material is made available only to registered students of the Degree Course in Molecular Biotechnology and Bioinformatics and should not be distributed to others.
Modalità di verifica dell’apprendimento e criteri di valutazione
The exam is written with half of the evaluation based on open-answer questions (Pellicioli), and a half on multiple-choice questions (Biffo & Vaccari).The two parts can be taken separately in two distinct exam sessions, with Pellicioli's part as second. Questions attain to subjects discussed during the course and will concur to define the overall mark by weighting equally on both parts.
Part A (Biffo&Vaccari) consists in 33 multiple-choice questions (1 hour; 1 correct answer in 5 alternatives,1 point/correct; -0.2 point penalty).
The exam part B (Pellicioli) consists in a free-text elaboration (1 hour) to address one question, which is usually formulated in two correlated parts. Text elaboration allows students to show their ability to describe and critically comment (also by using schemes and graphical models) the theoretical bases and the experimental approaches learned during the course.
Part A (Biffo&Vaccari) consists in 33 multiple-choice questions (1 hour; 1 correct answer in 5 alternatives,1 point/correct; -0.2 point penalty).
The exam part B (Pellicioli) consists in a free-text elaboration (1 hour) to address one question, which is usually formulated in two correlated parts. Text elaboration allows students to show their ability to describe and critically comment (also by using schemes and graphical models) the theoretical bases and the experimental approaches learned during the course.
BIO/06 - ANATOMIA COMPARATA E CITOLOGIA
BIO/11 - BIOLOGIA MOLECOLARE
BIO/11 - BIOLOGIA MOLECOLARE
Lectures: 80 ore
Siti didattici
Docente/i
Ricevimento:
Mercoledi 10.30-12.30 e Venerdi 10.30-11.30
Ufficio Via Celoria 26 Torre A Piano 4