Etiopathogenesis of hereditary and parasitic diseases

Unit H52-79-A: Genomic analysis of inherited diseases (Prof.Longeri)
LECTURES:
- Genetic and physical maps of domestic animals (2 hs).
- Whole genome reference sequences (1h)
- Nucleotide sequencing: Sanger and High throughput Sequencing methods (3 hs)
- Genome-wide SNP detection (for microarray) (1h)
- Genotyping by Sequencing (1h)
- Genotyping by hybridization (microarray) and by whole genome sequencing (1h)
- Linkage/Association analyses using genomic SNP genotypes: family (HM) and cases/controls studies (GWAS) (2h)
- Genomic variability and host-parasite interaction (1 hs)

PRACTICALS:
- Online practice on nucleic acid quantification for genomic analyses. In silico PCR. On line practice on Sanger sequencing (gDNA and mtDNA). Online practice on Genomic databases (6).
- Visits to research and service centres equipped with high-throughput platforms when available /or computer practice for WGS and GWAS analyses (4).
- Journal clubs on genome sequencing projects in domestic animals, gene mapping and identification in inherited diseases and parasitic diseases, the latter limited to those addressed in the lectures (2).

Unit H52-79-B: Molecular pathology of parasitic diseases (Prof.Mortarino).
LECTURES:
Unit 1 - Leishmaniasis: immunopathology and primary animal models (4hs)
Unit 2 - Individual and social honey bee immunity against parasites (2hs)
Unit 3 - Moonlighting proteins of parasites (2hs)
Unit 4 - Neospora and Toxoplasma - Pathology and host range determinants (2hs)
Unit 5 - Drug targets of parasites - insights from genome mining (2hs)
PRACTICALS:
Unit 1 - MicroRNA web resources (4hs)
Unit 2 - MicroRNA analysis - RNA extraction and retrotranscription (4hs)
Unit 3 - MicroRNA analysis - Quantitation (4hs)