High-throughput_screenings
A.A. 2023/2024
Obiettivi formativi
Non definiti
Risultati apprendimento attesi
Non definiti
Periodo: Primo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento può essere seguito come corso singolo.
Programma e organizzazione didattica
Edizione unica
Programma
· The basis of High throughput screening:
o Target identification and Assay development
o Automation and miniaturization, liquid handling,
o Detection and data analysis
· Computational high throughput screening
· Biochemical High throughput screening
· Phenotypic screenings and cell-based high throughput screening
o High-content versus high-throughput
· In vivo based high throughput screening
· Small molecule-based screenings and drug discovery in academy vs an industrial environment
· Genetic high throughput screenings
o RNAi/shRNA-based screenings
o CRISPR-Cas9 based screenings
· New approaches:
o iPSC and whole organ modeling
o cancer-on-a-chip
o Target identification and Assay development
o Automation and miniaturization, liquid handling,
o Detection and data analysis
· Computational high throughput screening
· Biochemical High throughput screening
· Phenotypic screenings and cell-based high throughput screening
o High-content versus high-throughput
· In vivo based high throughput screening
· Small molecule-based screenings and drug discovery in academy vs an industrial environment
· Genetic high throughput screenings
o RNAi/shRNA-based screenings
o CRISPR-Cas9 based screenings
· New approaches:
o iPSC and whole organ modeling
o cancer-on-a-chip
Prerequisiti
Non sono richieste conoscenze preliminari.
Metodi didattici
Alle lezioni frontali saranno affiancate esercitazioni, lavori di gruppo e spazi di riflessione e discussione.
Materiale di riferimento
We will not use a main textbook, but provide the students with:
· All of the slides presented during the course
· PDF version of all of the articles/reviews presented during the course
· Web addresses of all of the relevant sites (presented during the course)
· All of the slides presented during the course
· PDF version of all of the articles/reviews presented during the course
· Web addresses of all of the relevant sites (presented during the course)
Modalità di verifica dell’apprendimento e criteri di valutazione
-Written test (50% of the evaluation), with 2 open questions regarding the topics presented in the course.
-During the course, the students will be divided in small teams (2-3) to prepare journal clubs which will be discussed with the teacher (50% of the evaluation).
Results will be communicated to the students individually by e-mail. The test can be discussed with the Course Supervisor, by appointment.
-During the course, the students will be divided in small teams (2-3) to prepare journal clubs which will be discussed with the teacher (50% of the evaluation).
Results will be communicated to the students individually by e-mail. The test can be discussed with the Course Supervisor, by appointment.
BIO/11 - BIOLOGIA MOLECOLARE
MED/04 - PATOLOGIA GENERALE
MED/04 - PATOLOGIA GENERALE
Esercitazioni: 16 ore
Lezioni: 40 ore
Lezioni: 40 ore
Siti didattici
Docente/i
Ricevimento:
Previo appuntamento da concordare via e-mail
Campus IEO, Via Adamello, 16 Milano