High-Throughput Screenings
A.Y. 2023/2024
Learning objectives
Undefined
Expected learning outcomes
Undefined
Lesson period: First semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course can be attended as a single course.
Course syllabus and organization
Single session
Course syllabus
· The basis of High throughput screening:
o Target identification and Assay development
o Automation and miniaturization, liquid handling,
o Detection and data analysis
· Computational high throughput screening
· Biochemical High throughput screening
· Phenotypic screenings and cell-based high throughput screening
o High-content versus high-throughput
· In vivo based high throughput screening
· Small molecule-based screenings and drug discovery in academy vs an industrial environment
· Genetic high throughput screenings
o RNAi/shRNA-based screenings
o CRISPR-Cas9 based screenings
· New approaches:
o iPSC and whole organ modeling
o cancer-on-a-chip
o Target identification and Assay development
o Automation and miniaturization, liquid handling,
o Detection and data analysis
· Computational high throughput screening
· Biochemical High throughput screening
· Phenotypic screenings and cell-based high throughput screening
o High-content versus high-throughput
· In vivo based high throughput screening
· Small molecule-based screenings and drug discovery in academy vs an industrial environment
· Genetic high throughput screenings
o RNAi/shRNA-based screenings
o CRISPR-Cas9 based screenings
· New approaches:
o iPSC and whole organ modeling
o cancer-on-a-chip
Prerequisites for admission
No prior knowledge is required.
Teaching methods
Lectures will be accompanied by practicals, group work and time for reflection and collective discussion.
Teaching Resources
We will not use a main textbook, but provide the students with:
· All of the slides presented during the course
· PDF version of all of the articles/reviews presented during the course
· Web addresses of all of the relevant sites (presented during the course)
· All of the slides presented during the course
· PDF version of all of the articles/reviews presented during the course
· Web addresses of all of the relevant sites (presented during the course)
Assessment methods and Criteria
-Written test (50% of the evaluation), with 2 open questions regarding the topics presented in the course.
-During the course, the students will be divided in small teams (2-3) to prepare journal clubs which will be discussed with the teacher (50% of the evaluation).
Results will be communicated to the students individually by e-mail. The test can be discussed with the Course Supervisor, by appointment.
-During the course, the students will be divided in small teams (2-3) to prepare journal clubs which will be discussed with the teacher (50% of the evaluation).
Results will be communicated to the students individually by e-mail. The test can be discussed with the Course Supervisor, by appointment.
BIO/11 - MOLECULAR BIOLOGY
MED/04 - EXPERIMENTAL MEDICINE AND PATHOPHYSIOLOGY
MED/04 - EXPERIMENTAL MEDICINE AND PATHOPHYSIOLOGY
Practicals: 16 hours
Lessons: 40 hours
Lessons: 40 hours
Educational website(s)
Professor(s)