Functional genomics and the molecular basis of differentiation
A.A. 2025/2026
Obiettivi formativi
The objective of the course is to provide students with: 1) a general overview on the principal methods for the study of bacterial genomes (genome sequencing techniques, genome annotation, phylogenetic studies); 2) a general overview on metagenomics and meta-trancriptomics analyses; 3) a practical approach for gene analyses, sequences alignment and comparison, use of the principal data bases, phylogenetic reconstructions.
In addition, the course provides students with information and training in the advances in the understanding of the molecular basis of genomic and epigenetic mechanisms in gene expression in livestock species. In detail the objective of the course is to provide students with 1) a general overview of the OMIC technologies used to study the primary structure of DNA, 2) detailed knowledge of the use of OMIC technologies in epigenetics 3) a general overview of epigenetics and role of epigenetic changes in development and cell differentiation
In addition, the course provides students with information and training in the advances in the understanding of the molecular basis of genomic and epigenetic mechanisms in gene expression in livestock species. In detail the objective of the course is to provide students with 1) a general overview of the OMIC technologies used to study the primary structure of DNA, 2) detailed knowledge of the use of OMIC technologies in epigenetics 3) a general overview of epigenetics and role of epigenetic changes in development and cell differentiation
Risultati apprendimento attesi
At the end of the course, the student will be able to plan experiments for the study of microbial communities, genomes sequencing and annotation and phylogenetic relationship between organisms. The practical approach of the course will introduce the student to bioinformatics with the use of tools capable to study genomes and the interaction between organisms.
In addition, the students will acquire knowledge on epigenetics changes on DNA and their effect on gene regulation and cell differentiation. Furthermore, the students will have a detailed overview on the new techniques applied to epigenetic profiling and acquire expertise on ENCODE database.
In addition, the students will acquire knowledge on epigenetics changes on DNA and their effect on gene regulation and cell differentiation. Furthermore, the students will have a detailed overview on the new techniques applied to epigenetic profiling and acquire expertise on ENCODE database.
Periodo: Primo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento non può essere seguito come corso singolo. Puoi trovare gli insegnamenti disponibili consultando il catalogo corsi singoli.
Programma e organizzazione didattica
Edizione unica
Responsabile
Periodo
Primo semestre
Programma
Genomics and molecular basis of differentiation in model invertebrate organisms and pathogens
Frontal lessons
Metagenomics and metatrascrittomics: 4 hours
Alpha and beta diversity applied to the study of a microbic population: 2 hours
Phylogeny to study the molecular basis of differentiation: 4 hours
Using phylogeny to reconstruct nosocomial outbreaks: 2 hours
Practical lessons:
An overview on the principal molecular biology methods: 4 hours
Genome sequencing: 2 hours
The study of the molecular mechanisms of the interaction among symbiont-parasite-vertebrate host: 2 hours
Study of the microbial community of the tick Ixodes ricinus: the case of its endosybiont Midichloria mitochondrii: 4 hours
Next Generation Sequencing approaches and bioinformatics for genomics: 4 hours
Practical exercise: genes alignment, multi-locus sequence typing, tools for phylogenetic reconstruction, outbreak reconstruction: 8 ore
Genomics and epigenetics in vertebrates
Frontal lessons
Introduction to OMIC technologies in Livestock (2h)
Fundamentals of DNA, Chromosomes, Genes in Cell Division and Cell Cycle (2h)
Fundamentals of Gene Structure, Gene Expression: RNA genes and Non coding RNA(2h)
Next Generation sequencing applied DNA and RNA (4h)
Principles of Genetic Variation: functional genetic variation and protein polymorphism(2h)
Approaches to Mapping and Identifying Genetic Susceptibility to Complex traits(2h)
Epigenetic Theory and Principles of Gene Regulation and Epigenetics (2h)
Epigenetics of Mammalian gamete and embryo development(2h)
Practical lessons:
Analysis of the main databases of epigenetic and functional genomic interest (4 hours)
Bioinformatics exercises using the main bioinformatics tools for epigenetics and functional genomics (4 hours)
Examples of epigenetic modifications and modifications linked to functional genomics in the animal field (4 hours)
Frontal lessons
Metagenomics and metatrascrittomics: 4 hours
Alpha and beta diversity applied to the study of a microbic population: 2 hours
Phylogeny to study the molecular basis of differentiation: 4 hours
Using phylogeny to reconstruct nosocomial outbreaks: 2 hours
Practical lessons:
An overview on the principal molecular biology methods: 4 hours
Genome sequencing: 2 hours
The study of the molecular mechanisms of the interaction among symbiont-parasite-vertebrate host: 2 hours
Study of the microbial community of the tick Ixodes ricinus: the case of its endosybiont Midichloria mitochondrii: 4 hours
Next Generation Sequencing approaches and bioinformatics for genomics: 4 hours
Practical exercise: genes alignment, multi-locus sequence typing, tools for phylogenetic reconstruction, outbreak reconstruction: 8 ore
Genomics and epigenetics in vertebrates
Frontal lessons
Introduction to OMIC technologies in Livestock (2h)
Fundamentals of DNA, Chromosomes, Genes in Cell Division and Cell Cycle (2h)
Fundamentals of Gene Structure, Gene Expression: RNA genes and Non coding RNA(2h)
Next Generation sequencing applied DNA and RNA (4h)
Principles of Genetic Variation: functional genetic variation and protein polymorphism(2h)
Approaches to Mapping and Identifying Genetic Susceptibility to Complex traits(2h)
Epigenetic Theory and Principles of Gene Regulation and Epigenetics (2h)
Epigenetics of Mammalian gamete and embryo development(2h)
Practical lessons:
Analysis of the main databases of epigenetic and functional genomic interest (4 hours)
Bioinformatics exercises using the main bioinformatics tools for epigenetics and functional genomics (4 hours)
Examples of epigenetic modifications and modifications linked to functional genomics in the animal field (4 hours)
Prerequisiti
Knowledge required for the access to the Degree Course.
Metodi didattici
Genomics and molecular basis of differentiation in model invertebrate organisms and pathogens
Lectures, practical laboratory activities, group work.
Genomics and epigenetics in vertebrates
Lectures, practical bioinformatic activities and group works.
Lectures, practical laboratory activities, group work.
Genomics and epigenetics in vertebrates
Lectures, practical bioinformatic activities and group works.
Materiale di riferimento
Genomics and molecular basis of differentiation in model invertebrate organisms and pathogens
Material provided by the teacher and uploaded to the MyAriel platform.
Genomics and epigenetics in vertebrates
· Material provided by the teacher and uploaded to the My Ariel platform.
· Genetics and Genomics in Medicine , Tom Strachan, Judith Goodship, and Patrick Chinnery. Garland Science.
· Epigenetica, Massimo Ranieri, Edizioni Zanichelli 2021
Material provided by the teacher and uploaded to the MyAriel platform.
Genomics and epigenetics in vertebrates
· Material provided by the teacher and uploaded to the My Ariel platform.
· Genetics and Genomics in Medicine , Tom Strachan, Judith Goodship, and Patrick Chinnery. Garland Science.
· Epigenetica, Massimo Ranieri, Edizioni Zanichelli 2021
Modalità di verifica dell’apprendimento e criteri di valutazione
Genomics and molecular basis of differentiation in model invertebrate organisms and pathogens
The learning assessment will be carried out through a written test: 15 multiple choice questions. The test aims at ascertaining the acquisition of knowledge on the topics covered in the course during both theoretical lessons and practical exercises. Time for the test: 1 hour
Genomics and epigenetics in vertebrates
Written test. The obtainment of the expected learning outcomes by the student are assessed by a written examination based on questions with open answers which include also the description of the practical activity. The test will be graded out of thirty and the final grade will take into account the accuracy and quality of the answers. Time for the test: 1 hour.
The final grade will be an average of the two grades obtained. The exam is considered passed when the student has achieved a grade of at least 18/30 on both modules.
The learning assessment will be carried out through a written test: 15 multiple choice questions. The test aims at ascertaining the acquisition of knowledge on the topics covered in the course during both theoretical lessons and practical exercises. Time for the test: 1 hour
Genomics and epigenetics in vertebrates
Written test. The obtainment of the expected learning outcomes by the student are assessed by a written examination based on questions with open answers which include also the description of the practical activity. The test will be graded out of thirty and the final grade will take into account the accuracy and quality of the answers. Time for the test: 1 hour.
The final grade will be an average of the two grades obtained. The exam is considered passed when the student has achieved a grade of at least 18/30 on both modules.
AGR/17 - ZOOTECNICA GENERALE E MIGLIORAMENTO GENETICO - CFU: 4
VET/06 - PARASSITOLOGIA E MALATTIE PARASSITARIE DEGLI ANIMALI - CFU: 4
VET/06 - PARASSITOLOGIA E MALATTIE PARASSITARIE DEGLI ANIMALI - CFU: 4
Esercitazioni: 36 ore
Lezioni: 30 ore
Lezioni: 30 ore
Docente/i
Ricevimento:
Tutti i giorni su appuntamento
Dipartimento di Medicina Veterinaria e Scienze Animali
Ricevimento:
Disponibilità previo appuntamento tramite e-mail
Dipartimento di Medicina Veterinaria e Scienze Animali (DIVAS)