Molecular and cellular imaging
A.A. 2025/2026
Obiettivi formativi
The aim of the course is to present different techniques of microscopy (optical, electronic and scanning probe) also applied in lab-on-a-chip systems.
After following this course, the students will acquire basic know-how in microscopy and the capability to select suitable approaches to image biological samples.
The course is ideally linked to those dealing with the need of imaging, i.e. molecular and cellular biology, etc.
After following this course, the students will acquire basic know-how in microscopy and the capability to select suitable approaches to image biological samples.
The course is ideally linked to those dealing with the need of imaging, i.e. molecular and cellular biology, etc.
Risultati apprendimento attesi
After following this course, the students will acquire basic know-how in microscopy and the capability to select suitable approaches to image biological samples.
Periodo: Primo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento può essere seguito come corso singolo.
Programma e organizzazione didattica
Edizione unica
Responsabile
Periodo
Primo semestre
Programma
Part 1
The first part of the course provides a description of the imaging techniques applied to biology. Some basic notions of geometrical optics are functional to the detailed description of optical microscopes. Confocal microscopy and related techniques (FRAP, FRET, FLIM) will be extensively treated together with more recent developments in super-resolution techniques, in particular SIM, PALM, STORM, STED. Electron microscopies (SEM, TEM, Cryo-TEM) will be presented underlying the different investigations on biological samples that can be carried out. Atomic Force Microscopy (AFM) will be also presented as a versatile approach for measuring mechanical properties of biological samples.
Part 2
The second part of the course deals with spectroscopic techniques (FTIR, UV-Vis) for characterization of biological samples and with in vitro quantitative cell and tissue biology. An overview of Lab-on-chip (LOC) devices for laboratory functionalities integrated into miniaturized devices. In the end, some examples of engineered 3D cell cultures will be deeply discussed and practical problems will be presented.
The first part of the course provides a description of the imaging techniques applied to biology. Some basic notions of geometrical optics are functional to the detailed description of optical microscopes. Confocal microscopy and related techniques (FRAP, FRET, FLIM) will be extensively treated together with more recent developments in super-resolution techniques, in particular SIM, PALM, STORM, STED. Electron microscopies (SEM, TEM, Cryo-TEM) will be presented underlying the different investigations on biological samples that can be carried out. Atomic Force Microscopy (AFM) will be also presented as a versatile approach for measuring mechanical properties of biological samples.
Part 2
The second part of the course deals with spectroscopic techniques (FTIR, UV-Vis) for characterization of biological samples and with in vitro quantitative cell and tissue biology. An overview of Lab-on-chip (LOC) devices for laboratory functionalities integrated into miniaturized devices. In the end, some examples of engineered 3D cell cultures will be deeply discussed and practical problems will be presented.
Prerequisiti
None
Metodi didattici
All the theoretical and practical lectures will be delivered in the class-room. Some visits to imaging platform will be scheduled.
For students abroad the lessons will be synchronously delivered on ZOOM platform.
Archive and Notices on MyAriel.
For students abroad the lessons will be synchronously delivered on ZOOM platform.
Archive and Notices on MyAriel.
Materiale di riferimento
1] G. Haugstad, Atomic force microscopy: understanding basic modes and advanced applications, John Wiley & Sons, Hoboken, N.J, 2012.
[2] U. Kubitscheck, ed., Fluorescence microscopy: from principles to biological applications, Wiley-Blackwell, Weinheim, 2013.
[3] R.F. Egerton, Physical principles of electron microscopy: an introduction to TEM, SEM, and AEM, second edition, Springer, Cham, 2016.
[4] Tissue Engineering, Edited By Bernhard Palsson, Jeffrey A. Hubbell, Robert Plonsey, Joseph D. Bronzino, CRC Press 2019.
Copies of the slides projected in the classroom as well as other materials will be made available through the course website on the MyAriel platform of the University of Milano (https://clenardib.ariel.ctu.unimi.it/v5/home/Default.aspx). By no means this material replaces the lectures or a textbook. The material is made available only to registered students of the Degree Course in Molecular Biotechnology and Bioinformatics and should not be distributed to others.
[2] U. Kubitscheck, ed., Fluorescence microscopy: from principles to biological applications, Wiley-Blackwell, Weinheim, 2013.
[3] R.F. Egerton, Physical principles of electron microscopy: an introduction to TEM, SEM, and AEM, second edition, Springer, Cham, 2016.
[4] Tissue Engineering, Edited By Bernhard Palsson, Jeffrey A. Hubbell, Robert Plonsey, Joseph D. Bronzino, CRC Press 2019.
Copies of the slides projected in the classroom as well as other materials will be made available through the course website on the MyAriel platform of the University of Milano (https://clenardib.ariel.ctu.unimi.it/v5/home/Default.aspx). By no means this material replaces the lectures or a textbook. The material is made available only to registered students of the Degree Course in Molecular Biotechnology and Bioinformatics and should not be distributed to others.
Modalità di verifica dell’apprendimento e criteri di valutazione
The exam consists of:
1) oral exam concerning the topics covered in the course, in particular a part will deal with the principles and istruments for bioimaging, the spectral techniques for characterization of biological samples and Lab-On-Chip systems.
2) presentation with slides of a paper assigned by the teachers concerning recent studies in the biotechnology field in which at least one of the imaging techniques described during the course are used (max 20 min).
The exam will be in person. For students abroad specific arrangements will be taken according with the teacher.
1) oral exam concerning the topics covered in the course, in particular a part will deal with the principles and istruments for bioimaging, the spectral techniques for characterization of biological samples and Lab-On-Chip systems.
2) presentation with slides of a paper assigned by the teachers concerning recent studies in the biotechnology field in which at least one of the imaging techniques described during the course are used (max 20 min).
The exam will be in person. For students abroad specific arrangements will be taken according with the teacher.
FIS/03 - FISICA DELLA MATERIA - CFU: 3
FIS/07 - FISICA APPLICATA (A BENI CULTURALI, AMBIENTALI, BIOLOGIA E MEDICINA) - CFU: 3
FIS/07 - FISICA APPLICATA (A BENI CULTURALI, AMBIENTALI, BIOLOGIA E MEDICINA) - CFU: 3
Lectures: 48 ore
Docente:
Lenardi Cristina
Docente/i
Ricevimento:
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