Omics: from bench to bedside
A.A. 2025/2026
Obiettivi formativi
The aim of the course is to present to the students the fundamental logic in the use of omic methodologies, to introduce the theoretical and practical bases and the principles on which the different omic techniques are based, to describe the main protocols of use both in the preparation of the sample and of the instrumentation and their control, and finally to provide the basis for the management and analysis of the results including the possibility of integration of the data obtained with different omic methodologies.
In addition, since omics-based recent approaches have unveiled the molecular mechanism behind tumorigenesis of several human tumors and promoted the identification of next-generation molecular markers for early diagnosis, prognosis and even personalized targeted therapy, the course will introduce the student to the most promising omics approaches and their outcomes for the diagnosis and therapy of cancer.
In addition, since omics-based recent approaches have unveiled the molecular mechanism behind tumorigenesis of several human tumors and promoted the identification of next-generation molecular markers for early diagnosis, prognosis and even personalized targeted therapy, the course will introduce the student to the most promising omics approaches and their outcomes for the diagnosis and therapy of cancer.
Risultati apprendimento attesi
At the end of the course, the students will be able to address different scientific issues and to choose and apply the best omics approaches to understand the biochemical bases of tissue functions in health and disease. The student will be prepared to discuss the available modern high technologies to identify novel tumor markers and understand personalized therapies; they will learn the "omics" work flow from different clinical matrices (i.e. tumor specimen) to data analysis.
Periodo: Secondo semestre
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento non può essere seguito come corso singolo. Puoi trovare gli insegnamenti disponibili consultando il catalogo corsi singoli.
Programma e organizzazione didattica
Edizione unica
Responsabile
Periodo
Secondo semestre
Programma
The following topics will be explained during the teaching unit "Omics technology and approaches":
- An overview on Precision Medicine
- Large Scale Genome Sequencing Project
- Next Generation Sequencing Platforms
- Next Generation Sequencing libraries preparations and sequencing
- Next Generation Sequencing Applications: Whole Exome Sequencing (WES) and Whole Genome Sequencing (WGS)
- Next Generation Sequencing Applications: ATAC-seq, ChIP-seq, bulk-RNA-seq
- Spatial Transcriptomics
- Single Cell RNA-seq
- Introduction to proteomics
- Proteomic platform and instrumentation (high-resolution nanoLC-MS/MS system)
- Quantitative analysis in proteomics: Labelled and label-free approaches
- Imaging proteomics and applications
- Structural proteomics
- Introduction to metabolomics
- Metabolic platform: targeted vs untargeted metabolomics
- Lipidomics
- Fluxomics
- Introduction to bioinformatics
- Omics data integration
- Principle of system biology and application to disease
The following topics will be covered during the teaching unit "Omics approaches in diagnosis and therapy":
- Clinical applications of omics technologies in disease diagnosis, prognosis and therapy, personalized and precision medicine, molecular biomarkers
- Omics-based approaches in prenatal screening and developmental disorders
- Omics-based approaches in rare disease
- Omics-based approaches in cardiovascular diseases
- Omics-based approaches in cancer
- Omics-based approaches in neurodegenerative disorders
- Ethical and social considerations in clinical omics
- Experimental data obtained from animal models their relevance to disease.
- An overview on Precision Medicine
- Large Scale Genome Sequencing Project
- Next Generation Sequencing Platforms
- Next Generation Sequencing libraries preparations and sequencing
- Next Generation Sequencing Applications: Whole Exome Sequencing (WES) and Whole Genome Sequencing (WGS)
- Next Generation Sequencing Applications: ATAC-seq, ChIP-seq, bulk-RNA-seq
- Spatial Transcriptomics
- Single Cell RNA-seq
- Introduction to proteomics
- Proteomic platform and instrumentation (high-resolution nanoLC-MS/MS system)
- Quantitative analysis in proteomics: Labelled and label-free approaches
- Imaging proteomics and applications
- Structural proteomics
- Introduction to metabolomics
- Metabolic platform: targeted vs untargeted metabolomics
- Lipidomics
- Fluxomics
- Introduction to bioinformatics
- Omics data integration
- Principle of system biology and application to disease
The following topics will be covered during the teaching unit "Omics approaches in diagnosis and therapy":
- Clinical applications of omics technologies in disease diagnosis, prognosis and therapy, personalized and precision medicine, molecular biomarkers
- Omics-based approaches in prenatal screening and developmental disorders
- Omics-based approaches in rare disease
- Omics-based approaches in cardiovascular diseases
- Omics-based approaches in cancer
- Omics-based approaches in neurodegenerative disorders
- Ethical and social considerations in clinical omics
- Experimental data obtained from animal models their relevance to disease.
Prerequisiti
A basic knowledge of Cellular Biology, Biochemistry, Applied Biochemistry, Molecular Biology, Immunology, and Human Physiopathology is strongly recommended for the Omics Technology and Approaches teaching unit.
Metodi didattici
The course is composed of two teaching units: 1. "Omics technology and approaches" (4 ETCS, 32 hours) and 2. "Omics approaches in diagnosis and therapy" (2 ETCS, 16 hours). Both teaching units consist of lectures given by the Teachers and, occasionally, seminars by external experts on particular topics.
Powerpoint presentations or short videos are usually used and are provided to the students on the Ariel site of the course.
Powerpoint presentations or short videos are usually used and are provided to the students on the Ariel site of the course.
Materiale di riferimento
For both units the iconographic materials shown during the lessons and bibliographic references can be downloaded from the Ariel portal.
Modalità di verifica dell’apprendimento e criteri di valutazione
The students' learning outcomes will be evaluated by a written examination on the omics methodologies and their applications, according to the program covered during both teaching units: "Omics technology and approaches" and "Omics approaches in diagnosis and therapy". Specifically, the written exam will consist of multiple-choice questions based on the topics addressed during the course. The exam for both teaching units will be held on the same day, therefore the students must take both units at once.
The final grade will be the weighted average of the grades obtained in "Omics technology and approaches" and "Omics approaches in diagnosis and therapy" exams, based on the credits assigned to each teaching unit.
The final grade will be the weighted average of the grades obtained in "Omics technology and approaches" and "Omics approaches in diagnosis and therapy" exams, based on the credits assigned to each teaching unit.
BIO/10 - BIOCHIMICA - CFU: 4
MED/04 - PATOLOGIA GENERALE - CFU: 2
MED/04 - PATOLOGIA GENERALE - CFU: 2
Lezioni: 48 ore
Docenti:
Cattaneo Paola Luisa, Mitro Nico
Docente/i
Ricevimento:
Su appuntamento telefonico o e-mail
Via Balzaretti 9, 20113 Milano