Imaging techniques in bio-medical research

A.Y. 2018/2019
8
Max ECTS
48
Overall hours
SSD
BIO/10 VET/01
Language
English
Learning objectives
L'analisi per immagini rappresenta un campo emergente delle scienze biomediche e ciò è dimostrato dal numero crescente di corsi disponibili presso prestigiose università nel mondo, dall'offerta di opportunità di impiego e dal sempre maggior ricorrere di queste tecnologie nelle pubblicazioni scientifiche del settore biotecnologico e biomedico. Con la frequenza del corso, lo studente impara che è possibile estendere il suo orizzonte di approccio biotecnologico mediante la raccolta di dati biologici di primaria importanza, ottenuti e rielaborati mediante l'applicazione di principi fisici e matematici di elevata precisione e riproducibilità. I dati sono poi rielaborati e processati per poter essere interpretati opportunamente.
Expected learning outcomes
Undefined
Course syllabus and organization

Single session

Responsible
Lesson period
Second semester
ATTENDING STUDENTS
Module: Image analysis of cells and tissue
Course syllabus
Basic concepts in imaging. Electromagnetic radiation and interactions with Matter (2 hours),
Optics, including lenses and reflection. Generators, filters and detectors (1 hour)
Biological effects of radiation. Safety considerations (1 hour).
Imaging systems for biological molecole (Proteins, lipids, nucleic acids) (2 hours)
Trasmission microscopy (1 hour)
Scanning microscopy (1 hour)
Phase contrast microscopy (1 hour)
Fluorescence microscopy (1 hour)
Confocal and multiphoton microscopy (2 hours)
Imaging of live and fixed cells (2 hours)
Fluorescence Resonance Energy Transfer (2 hours)
Use of radioisotopes for imaging con radionuclide (1 hour)
Magnetic resonance (1 hour)

Practical training total hours

Time-lapse of living systems 2
Use of algorithms used in imaging 1
Processing, digitalization deconvolution 1
Image degradation, Spatial resolution, Signal and noise 1
Image measurements, histograms 1
Use of Image 1.62 NIH program 2
Use of Leica Confocal System 2
Use of Nikon NKM Program 2
Module: MALDI imaging
Course syllabus
Lectures total hours
Sample preparation 4
MALDI imaging 4
Proteins identification and analysis 4
Module: Molecular Imaging
Course syllabus
Preliminary overview 4
Relaxation Theory and Mechanisms
Magnetic properties and image effects
Extracellular fluid agents 2
Organ-specific agents 2
Ultrasound theory and demonstration
Scintigraphy and Tomography scanning 2
Bioluminescent imaging and the use of reporter in vivo 2
NON-ATTENDING STUDENTS
Module: Image analysis of cells and tissue
Course syllabus
Basic concepts in imaging. Electromagnetic radiation and interactions with Matter (2 hours),
Optics, including lenses and reflection. Generators, filters and detectors (1 hour)
Biological effects of radiation. Safety considerations (1 hour).
Imaging systems for biological molecole (Proteins, lipids, nucleic acids) (2 hours)
Trasmission microscopy (1 hour)
Scanning microscopy (1 hour)
Phase contrast microscopy (1 hour)
Fluorescence microscopy (1 hour)
Confocal and multiphoton microscopy (2 hours)
Imaging of live and fixed cells (2 hours)
Fluorescence Resonance Energy Transfer (2 hours)
Use of radioisotopes for imaging con radionuclide (1 hour)
Magnetic resonance (1 hour)

Practical training total hours

Time-lapse of living systems 2
Use of algorithms used in imaging 1
Processing, digitalization deconvolution 1
Image degradation, Spatial resolution, Signal and noise 1
Image measurements, histograms 1
Use of Image 1.62 NIH program 2
Use of Leica Confocal System 2
Use of Nikon NKM Program 2
Module: MALDI imaging
Course syllabus
Lectures total hours
Sample preparation 4
MALDI imaging 4
Proteins identification and analysis 4
Module: Molecular Imaging
Course syllabus
Preliminary overview 4
Relaxation Theory and Mechanisms
Magnetic properties and image effects
Extracellular fluid agents 2
Organ-specific agents 2
Ultrasound theory and demonstration
Scintigraphy and Tomography scanning 2
Bioluminescent imaging and the use of reporter in vivo 2
Module: Image analysis of cells and tissue
VET/01 - VETERINARY ANATOMY - University credits: 4
Lessons: 24 hours
Professor: Brevini Tiziana
Module: MALDI imaging
BIO/10 - BIOCHEMISTRY - University credits: 2
Lessons: 12 hours
Professor: Tedeschi Gabriella
Module: Molecular Imaging
VET/01 - VETERINARY ANATOMY - University credits: 2
Lessons: 12 hours
Professor: Brevini Tiziana